Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,994	T→C	M1T (ATG→ACG) †	scpB →	methylmalonyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,994	0	T	C	100.0%	168.2 / NA	46	M1T (ATG→ACG) †	scpB	methylmalonyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/25); total (21/25)

TTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAGCGATCTCA  >  NC_000913/3063857‑3064122

ttCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGt                                                                                                                                <  2:860523/140‑1 (MQ=255)

     gAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTAt                                                                                                                           <  2:54975/140‑1 (MQ=255)

       aTGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCa                                                                                                                         >  1:491323/1‑140 (MQ=255)

        tGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAg                                                                                                                        <  2:488696/140‑1 (MQ=255)

              tttCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGtt                                                                                                                  <  2:726563/140‑1 (MQ=255)

               ttCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTa                                                                                                                 <  2:855848/140‑1 (MQ=255)

                tCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTaa                                                                                                                <  2:297809/140‑1 (MQ=255)

                   aTCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGt                                                                                                             >  1:265645/1‑140 (MQ=255)

                       cTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTc                                                                                                         >  2:637823/1‑140 (MQ=255)

                       cTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTc                                                                                                         <  2:548302/140‑1 (MQ=255)

                                  aGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATcaacaa                                                                                              >  1:701712/1‑140 (MQ=255)

                                  aGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATcaacaa                                                                                              >  2:695842/1‑140 (MQ=255)

                                    aCGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAg                                                                                            >  2:628113/1‑140 (MQ=255)

                                     cGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGt                                                                                           <  1:637823/140‑1 (MQ=255)

                                               ggTCAAAAACAATACGCTCTCACCGCGCACCGACCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCAtt                                                                                 <  2:388558/140‑1 (MQ=255)

                                                 tCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCAtt                                                                                 >  1:548436/1‑138 (MQ=255)

                                                 tCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCAtt                                                                                 <  2:548436/138‑1 (MQ=255)

                                                  cAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAg                                                                              >  1:809567/1‑140 (MQ=255)

                                                   aaaaaCAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGt                                                                             <  2:192577/140‑1 (MQ=255)

                                                                 ctcACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGa                                                               <  2:491323/140‑1 (MQ=255)

                                                                  tcACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGaa                                                              <  1:245750/140‑1 (MQ=255)

                                                                   cACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGaaa                                                             >  2:825159/1‑140 (MQ=255)

                                                                       gcgcACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTTAATATAGCCCaaaaaat                                                         >  2:245750/1‑137 (MQ=255)

                                                                       gcgcACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACtt                                                         >  2:190010/1‑140 (MQ=255)

                                                                          cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGa                                                               >  1:75224/1‑131 (MQ=255)

                                                                          cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGa                                                               <  2:75224/131‑1 (MQ=255)

                                                                          cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTa                                                        <  2:172793/138‑1 (MQ=255)

                                                                          cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTa                                                        >  1:172793/1‑138 (MQ=255)

                                                                          cACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAAt                                                      >  1:36487/1‑140 (MQ=255)

                                                                             cGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGcc                                                   >  1:439805/1‑140 (MQ=255)

                                                                              ggCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCt                                                  <  2:439805/140‑1 (MQ=255)

                                                                               gCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCtt                                                 <  1:190010/140‑1 (MQ=255)

                                                                                  tGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAg                                              >  2:497931/1‑140 (MQ=255)

                                                                                      gCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTaaa                                          <  1:549116/140‑1 (MQ=255)

                                                                                        aGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGt                                        <  1:825159/140‑1 (MQ=255)

                                                                                          cTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCt                                      >  1:751039/1‑140 (MQ=255)

                                                                                           tCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCtt                                     <  1:497931/140‑1 (MQ=255)

                                                                                               tGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAAATATGGCCGAAAAATTAATGCCTTAAGTAAAAACTTTAtt                                 >  2:495677/1‑140 (MQ=255)

                                                                                                 aaTTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATtga                               <  1:495677/140‑1 (MQ=255)

                                                                                                 aaTTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATtga                               <  2:809567/140‑1 (MQ=255)

                                                                                                    ttATCCAGACGCAATATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTTACTATGGCCGAAAACTTAATGCCTTAAGTAAAATCTTTATtgatga                            >  1:834643/1‑140 (MQ=255)

                                                                                                                aaTATTTTGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTCAAGTAAAGTCTTTATTGATGATCTTATGCAGGc                <  2:701712/140‑1 (MQ=255)

                                                                                                                      ttGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAg          <  2:108127/140‑1 (MQ=255)

                                                                                                                      ttGATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAg          <  2:751039/140‑1 (MQ=255)

                                                                                                                        gATTAAAGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTTAGTTTTAATATGGCCGAAAAATTAATGACTTAAGTAAAGTCTTTATTGATGATCTTATTCAGGGGTTAAACg        >  1:204547/1‑140 (MQ=255)

                                                                                                                              aGGAATTTTTACGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGGGGTTAAAAATTGACCAAAAAATAAAGGCCTAAGATAAGGCTTTATTGATTATCTTATGCAAGAGTTCAGcactctaa  >  2:158926/1‑133 (MQ=255)

TTCGCGAATGAAGATTTCGATCGCTATTACCGCCAGACGCTTTTAGCGGTCAAAAACAATACGCTCTCACCGCGCACCGGCCTGCGGCAGCTCAGTGAATTTATCCAGACGCAATATTTTGATTAAAGGAATTTTTATGTCTTATCAGTATGTTAACGTTGTCACTATCAACAAAGTGGCGGTCATTGAGTTTAACTATGGCCGAAAACTTAATGCCTTAAGTAAAGTCTTTATTGATGATCTTATGCAGGCGTTAAGCGATCTCA  >  NC_000913/3063857‑3064122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A