Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,851,355	A→G	V477A (GTG→GCG)	ilvB ←	acetohydroxy acid synthase I subunit IlvB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,851,355	0	A	G	100.0%	153.1 / NA	42	V477A (GTG→GCG)	ilvB	acetohydroxy acid synthase I subunit IlvB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/22); total (20/22)

TGCGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGC  >  NC_000913/3851223‑3851491

tGCGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGccc                                                                                                                                   <  2:539175/140‑1 (MQ=255)

  cGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCa                                                                                                                                 <  2:617564/140‑1 (MQ=255)

     aTCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGTTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAgcg                                                                                                                              <  1:695395/140‑1 (MQ=255)

       cGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCt                                                                                                                            >  2:1033690/1‑140 (MQ=255)

        ggCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCtt                                                                                                                           >  2:97809/1‑140 (MQ=255)

        ggCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCtt                                                                                                                           <  2:308500/140‑1 (MQ=255)

        ggCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCtt                                                                                                                           <  1:521304/140‑1 (MQ=255)

             cGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCgttg                                                                                                                      <  2:130397/140‑1 (MQ=255)

             cGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCgttg                                                                                                                      <  1:437923/140‑1 (MQ=255)

                         cacaGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATg                                                                                                          >  2:875624/1‑140 (MQ=255)

                            aGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGAtt                                                                                                       >  2:528583/1‑140 (MQ=255)

                                 tCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGAc                                                                                                  >  1:502585/1‑140 (MQ=255)

                                 tCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGAc                                                                                                  >  1:515263/1‑140 (MQ=255)

                                 tCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGAc                                                                                                  >  1:17628/1‑140 (MQ=255)

                                      gCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCa                                                                                             >  1:944369/1‑140 (MQ=255)

                                       ccGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAg                                                                                            <  2:260966/140‑1 (MQ=255)

                                        cGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGc                                                                                           >  1:433099/1‑140 (MQ=255)

                                        cGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGc                                                                                           >  2:1045512/1‑140 (MQ=255)

                                          aaTCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTg                                                                                         >  1:149353/1‑140 (MQ=255)

                                          aaTCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTg                                                                                         >  1:708290/1‑140 (MQ=255)

                                           aTCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGa                                                                                        <  1:191701/140‑1 (MQ=255)

                                                      aTCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGc                                                                             >  1:198990/1‑140 (MQ=255)

                                                        cTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCgg                                                                           <  1:97809/140‑1 (MQ=255)

                                                        cTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCgg                                                                           <  2:708290/140‑1 (MQ=255)

                                                             tAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGcc                                                                      <  1:1045512/140‑1 (MQ=255)

                                                             tAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGcc                                                                      <  2:433099/140‑1 (MQ=255)

                                                             tAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGcc                                                                      <  2:184460/140‑1 (MQ=255)

                                                              aaaGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCa                                                                     >  1:184892/1‑140 (MQ=255)

                                                               aaGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCAt                                                                    <  2:184892/140‑1 (MQ=255)

                                                               aaGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCAt                                                                    <  2:449921/140‑1 (MQ=255)

                                                                           cccGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATAtt                                                        >  2:953774/1‑140 (MQ=255)

                                                                                         ggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGc                                          >  1:446779/1‑140 (MQ=255)

                                                                                         ggcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGc                                          <  2:515263/140‑1 (MQ=255)

                                                                                          gcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGcc                                         <  2:808860/140‑1 (MQ=255)

                                                                                          gcAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGcc                                         >  2:811855/1‑140 (MQ=255)

                                                                                                  gCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCgg                                 <  1:953774/140‑1 (MQ=255)

                                                                                                    cTTGCTCGTAGAACAGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGgag                               <  1:811855/140‑1 (MQ=255)

                                                                                                                  aGACTCTGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCCGAATATTTATCATCAGGCTGCCGTCGCCGGAGAAACACAAAACttt                 >  2:580641/1‑140 (MQ=255)

                                                                                                                     ctctGTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAAAACAACACTTTTCg              >  2:1027355/1‑140 (MQ=255)

                                                                                                                         gTTGCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCCTCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATcc          >  2:309507/1‑140 (MQ=255)

                                                                                                                            gCTGATGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCggg       <  2:446779/140‑1 (MQ=255)

                                                                                                                                 tGCGCCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGc  <  2:198990/140‑1 (MQ=255)

TGCGGATCGGCTTCGTTATTCAAATCACAGGTTTCGAGGCCGAATCCGGCGGCAATCTGCATAAAGTTGATTTTGCCCGGATAGGTGGCGGCAAAAACGCCTTGCTCGTAGAACAGACTCTGTTGCTGATGCACCAGCCCCAGCGCTTCGTTGTTCATCAGAATGATTTTGACATCCAGCTGATTTTCACTGGCGGTCGCCATCTCCTGAATATTCATCATCAGGCTGCCGTCGCCGGAGAAACACAACACTTTGCGATCCGGGTTCGC  >  NC_000913/3851223‑3851491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A