Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,923	A→G	intergenic (‑104/‑166)	ybjL ← / → ybjM	putative transport protein YbjL/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,923	0	A	G	100.0%	211.9 / NA	57	intergenic (‑104/‑166)	ybjL/ybjM	putative transport protein YbjL/putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/39); total (18/39)

CCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAATGT  >  NC_000913/889787‑890058

cccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGa                                                                                                                                      <  2:724005/140‑1 (MQ=255)

 ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                     >  2:1034637/1‑140 (MQ=255)

 ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                     <  1:697664/140‑1 (MQ=255)

 ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                     <  1:393965/140‑1 (MQ=255)

 ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                     >  2:667097/1‑140 (MQ=255)

   aTTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATag                                                                                                                                   >  2:567380/1‑140 (MQ=255)

         cAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                             <  2:950814/140‑1 (MQ=255)

         cAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                             <  1:642992/140‑1 (MQ=255)

              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                        <  2:497054/140‑1 (MQ=255)

              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                        <  2:273911/140‑1 (MQ=255)

              cGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACaa                                                                                                                        <  2:186952/140‑1 (MQ=255)

                  gACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAatata                                                                                                                    <  2:1260099/140‑1 (MQ=255)

                       ttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                               <  2:343881/140‑1 (MQ=255)

                          tatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTa                                                                                                            <  1:1384561/140‑1 (MQ=255)

                          tatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTa                                                                                                            <  2:215081/140‑1 (MQ=255)

                            ttCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAgt                                                                                                          <  2:288551/140‑1 (MQ=255)

                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGGTAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                     >  2:136361/1‑140 (MQ=255)

                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                     <  1:1406667/140‑1 (MQ=255)

                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                     >  2:900528/1‑140 (MQ=255)

                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                     >  1:1110114/1‑140 (MQ=255)

                                   aGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATca                                                                                                   >  1:469671/1‑140 (MQ=255)

                                     gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATcaca                                                                                                 <  2:1479249/140‑1 (MQ=255)

                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACAt                                                                                                <  1:667097/140‑1 (MQ=255)

                                         aCTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAg                                                                                             <  1:10767/140‑1 (MQ=255)

                                              ttGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGAATc                                                                                        <  1:1034637/140‑1 (MQ=255)

                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATc                                                                                        >  2:865417/1‑140 (MQ=255)

                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                       <  1:878071/140‑1 (MQ=255)

                                               tGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGACAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCa                                                                                       <  1:1397294/140‑1 (MQ=255)

                                                gTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCaa                                                                                      <  2:1217038/140‑1 (MQ=255)

                                                gTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTGTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCTCATTAGGTATCaa                                                                                      <  2:1518263/140‑1 (MQ=255)

                                                   tACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACgg                                                                                   >  1:415278/1‑140 (MQ=255)

                                                    aCTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGc                                                                                  <  1:48933/140‑1 (MQ=255)

                                                         tAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTatat                                                                             >  2:1428049/1‑140 (MQ=255)

                                                           aGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGa                                                                           >  1:996859/1‑140 (MQ=255)

                                                           aGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGa                                                                           >  1:466113/1‑140 (MQ=255)

                                                            ggagatGAAAGAAATGGTCCTTTACGTGCGTT‑TTTGAAACCAGAATACATTGAGCAGATTCATTCAGCGCACCTAAGGGATAGTATAGTAACAATATATTTTACTACTCCAATCACATTCGGGATCACGGTCTATAAGaa                                                                          <  2:573205/135‑1 (MQ=255)

                                                            gCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGaa                                                                          <  1:337346/140‑1 (MQ=255)

                                                                 tGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                     <  2:1169342/140‑1 (MQ=255)

                                                                 tGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                     <  1:567380/140‑1 (MQ=255)

                                                                  gaaagaaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATGTGAATTGCGt                                                                    <  1:979147/140‑1 (MQ=255)

                                                                  gaaagaaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                    <  1:618933/140‑1 (MQ=255)

                                                                    aagaaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTg                                                                  <  1:900528/140‑1 (MQ=255)

                                                                     agaaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTgg                                                                 <  2:915193/140‑1 (MQ=255)

                                                                      gaaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                                <  1:780515/140‑1 (MQ=255)

                                                                      gaaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                                <  1:68269/140‑1 (MQ=255)

                                                                       aaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGTGTTGGcc                                                               <  2:1440028/140‑1 (MQ=255)

                                                                       aaaTGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                               <  1:400151/140‑1 (MQ=255)

                                                                          tGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtat                                                            >  1:782409/1‑140 (MQ=255)

                                                                           ggTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTTCTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtata                                                           <  2:643710/140‑1 (MQ=255)

                                                                           ggTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtata                                                           <  1:139889/140‑1 (MQ=255)

                                                                                             ttttttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGaa                                          <  1:865417/140‑1 (MQ=255)

                                                                                                   aCCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCt                                    <  1:1428049/140‑1 (MQ=255)

                                                                                                             aGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACt                          <  2:1000417/140‑1 (MQ=255)

                                                                                                                                cgcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaa       >  1:264971/1‑140 (MQ=255)

                                                                                                                                cgcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaa       >  2:555963/1‑140 (MQ=255)

                                                                                                                                cgcACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGaaa       >  2:135951/1‑140 (MQ=255)

                                                                                                                                     cTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAAtgt  >  2:1223914/1‑140 (MQ=255)

                                                                                                                                     cTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAAtgt  >  1:1299968/1‑140 (MQ=255)

CCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATG‑TTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAATGT  >  NC_000913/889787‑890058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A