Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	952,685	A→G	R290R (CGT→CGC)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	952,685	0	A	G	100.0%	428.9 / NA	115	R290R (CGT→CGC)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (56/59); total (56/59)

Only 100 of 118 total aligned reads displayed.

TTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGACCAGAG  >  NC_000913/952549‑952820

ttGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGt                                                                                                                                      <  2:866463/140‑1 (MQ=255)

ttGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGt                                                                                                                                      <  1:496637/140‑1 (MQ=255)

ttGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGt                                                                                                                                      >  1:612847/1‑140 (MQ=255)

ttGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGt                                                                                                                                      <  2:781503/140‑1 (MQ=255)

 tGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGtt                                                                                                                                     <  2:546400/140‑1 (MQ=255)

   cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCg                                                                                                                                   >  1:612565/1‑140 (MQ=255)

    ccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGa                                                                                                                                  <  1:1084036/140‑1 (MQ=255)

     cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGAt                                                                                                                                 >  2:1242852/1‑140 (MQ=255)

        aTCGGGTCGCCAGATATCAGTTGATCGTATTACTGATTACGCATGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATGTCCTGCACATCTTGTCCGGTGATCTTGCCAGCATTCAGGTCGCGTTCGATGTa                                                                                                                              <  2:379165/140‑1 (MQ=255)

        aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGACCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTa                                                                                                                              <  2:600483/140‑1 (MQ=255)

        aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTa                                                                                                                              <  2:498167/140‑1 (MQ=255)

        aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGGCGCGTTCGATGTa                                                                                                                              >  2:825860/1‑140 (MQ=255)

         tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTac                                                                                                                             <  1:416745/140‑1 (MQ=255)

            ggTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTAcaca                                                                                                                          >  2:734553/1‑140 (MQ=255)

             gTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACAt                                                                                                                         >  1:908438/1‑140 (MQ=255)

              tCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATc                                                                                                                        >  2:1238116/1‑140 (MQ=255)

               cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATcc                                                                                                                       >  1:508060/1‑140 (MQ=255)

                  cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAgg                                                                                                                    >  1:342880/1‑140 (MQ=255)

                  cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAgg                                                                                                                    <  1:822267/140‑1 (MQ=255)

                       aaCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTATACATCCAGGAAGGt                                                                                                               >  1:268037/1‑140 (MQ=255)

                       aaCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGt                                                                                                               <  1:929974/140‑1 (MQ=255)

                           gTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAg                                                                                                           >  2:64374/1‑140 (MQ=255)

                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAgg                                                                                                          >  2:482557/1‑140 (MQ=255)

                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAgg                                                                                                          >  1:1332566/1‑140 (MQ=255)

                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAgg                                                                                                          >  2:1406995/1‑140 (MQ=255)

                            ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGGGGTCAACCATGTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCATGTCGCGGTCGATGTACACATCCAGGAAGACGGAgg                                                                                                          >  1:979139/1‑140 (MQ=255)

                              cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTa                                                                                                        <  2:465851/140‑1 (MQ=255)

                                tCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACg                                                                                                      >  2:244668/1‑140 (MQ=255)

                                 cGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGa                                                                                                     >  1:944147/1‑140 (MQ=255)

                                    aTTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCg                                                                                                  <  1:1191409/140‑1 (MQ=255)

                                      tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGaa                                                                                                >  2:534888/1‑140 (MQ=255)

                                      tCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGaa                                                                                                >  1:1061278/1‑140 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  2:1194777/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  1:396150/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  1:751353/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  1:1229601/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  2:1102485/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  1:964695/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGa                                                                                             <  1:890070/140‑1 (MQ=255)

                                         ggAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAATTCCTGCGCCTCTTGTGCGGTGATTGTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGGGGTACGACCGAAGGa                                                                                             <  2:1497229/140‑1 (MQ=255)

                                          gAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGAc                                                                                            >  1:591481/1‑140 (MQ=255)

                                             tACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACAtt                                                                                         >  1:885940/1‑140 (MQ=255)

                                               cGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgc                                                                                       >  2:864579/1‑140 (MQ=255)

                                                gCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgca                                                                                      >  1:1424022/1‑140 (MQ=255)

                                                gCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgca                                                                                      >  1:1126876/1‑140 (MQ=255)

                                                  aGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgcagc                                                                                    <  1:342982/140‑1 (MQ=255)

                                                   ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgcagca                                                                                   <  1:1238116/140‑1 (MQ=255)

                                                   ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgcagca                                                                                   <  2:235990/140‑1 (MQ=255)

                                                   ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgcagca                                                                                   <  1:825860/140‑1 (MQ=255)

                                                   ggAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTgcagca                                                                                   <  1:1018302/140‑1 (MQ=255)

                                                    gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCAc                                                                                  <  2:447026/140‑1 (MQ=255)

                                                    gAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCAc                                                                                  <  2:612847/140‑1 (MQ=255)

                                                       gCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGt                                                                               >  1:1090978/1‑140 (MQ=255)

                                                         gAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTc                                                                             >  2:1209797/1‑140 (MQ=255)

                                                          aaCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCt                                                                            >  1:1236849/1‑140 (MQ=255)

                                                           aCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTg                                                                           >  1:137317/1‑140 (MQ=255)

                                                           aCCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTg                                                                           <  1:258097/140‑1 (MQ=255)

                                                            ccATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTga                                                                          >  2:1054044/1‑140 (MQ=255)

                                                               tACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGAc                                                                       <  1:1386736/140‑1 (MQ=255)

                                                                    aGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTATACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAAc                                                                  <  2:268037/140‑1 (MQ=255)

                                                                       ttCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAAcagc                                                               >  1:409400/1‑140 (MQ=255)

                                                                         cATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAAcagcag                                                             <  2:1304335/140‑1 (MQ=255)

                                                                         cATGACCAGGCGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAAcagcag                                                             >  1:454467/1‑140 (MQ=255)

                                                                          aTGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACagcagc                                                            <  2:1332566/140‑1 (MQ=255)

                                                                           tGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGcc                                                           >  2:1529404/1‑140 (MQ=255)

                                                                           tGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGcc                                                           >  2:6032/1‑140 (MQ=255)

                                                                              ccAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAgg                                                        >  2:1143641/1‑140 (MQ=255)

                                                                              ccAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAgg                                                        <  2:591481/140‑1 (MQ=255)

                                                                                aGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAA‑‑‑‑‑‑‑GTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGaag                                               >  2:399580/1‑140 (MQ=255)

                                                                                  gtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGc                                                    <  2:508060/140‑1 (MQ=255)

                                                                                  gtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGc                                                    >  1:1284655/1‑140 (MQ=255)

                                                                                  gtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGc                                                    <  1:1242852/140‑1 (MQ=255)

                                                                                  gtggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGA‑TTAACAGCAGCCAGGTAGcc                                                   >  1:722316/1‑140 (MQ=255)

                                                                                    ggtCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCg                                                  >  2:348605/1‑140 (MQ=255)

                                                                                       cAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGaag                                               >  1:133269/1‑140 (MQ=255)

                                                                                         aCCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGaagta                                             >  2:853400/1‑140 (MQ=255)

                                                                                          ccATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGaagtaa                                            <  2:513707/140‑1 (MQ=255)

                                                                                             tttCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTc                                         >  1:1537826/1‑140 (MQ=255)

                                                                                             tttCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTc                                         <  2:1395770/140‑1 (MQ=255)

                                                                                               tCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCa                                       <  2:6174/140‑1 (MQ=255)

                                                                                                ccTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCAc                                      >  1:805718/1‑140 (MQ=255)

                                                                                                    cgcTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGa                                  >  1:760518/1‑140 (MQ=255)

                                                                                                     gcTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGAt                                 <  1:1441103/140‑1 (MQ=255)

                                                                                                     gcTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGAt                                 >  1:179411/1‑140 (MQ=255)

                                                                                                        tcttGTTCGGTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGc                              >  1:555224/1‑140 (MQ=255)

                                                                                                                ggTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAg                      <  2:1236849/140‑1 (MQ=255)

                                                                                                                 gTGATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGc                     >  2:538581/1‑140 (MQ=255)

                                                                                                                   gATCTTGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGt                   >  1:854651/1‑140 (MQ=255)

                                                                                                                       ttGCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGt               >  1:1444168/1‑140 (MQ=255)

                                                                                                                         gCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAg             >  2:1543372/1‑140 (MQ=255)

                                                                                                                         gCCAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAg             <  1:975683/140‑1 (MQ=255)

                                                                                                                          ccAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGc            <  1:1406995/140‑1 (MQ=255)

                                                                                                                          ccAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGc            <  1:252514/140‑1 (MQ=255)

                                                                                                                          ccAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGc            >  1:1123553/1‑140 (MQ=255)

                                                                                                                          ccAGCTTTCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTCGGTAGc            <  2:1502824/140‑1 (MQ=255)

                                                                                                                               tttCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGAc       <  2:885940/140‑1 (MQ=255)

                                                                                                                                ttCAGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGAcc      <  1:853400/140‑1 (MQ=255)

                                                                                                                                   aGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCGGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGACCaga   <  1:199073/140‑1 (MQ=255)

                                                                                                                                   aGGTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGACCaga   <  1:534888/140‑1 (MQ=255)

                                                                                                                                    ggTCGCGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGACCagag  <  2:760518/140‑1 (MQ=255)

TTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTTCTTGTTCGGTGATCTTGCCAGCTTTCAGGTCACGTTCGATGTACACATCCAGGAAGGTGGAGGTACGACCGAAGGACATTGCAGCACCGTTCTGAGACTTAACAGCAGCCAGGTAGCCGAAGTAAGTCCACTGGATAGCTTCCTGAGCGTTGGTAGCCGGACCAGAG  >  NC_000913/952549‑952820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A