Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	976,235	T→C	I205T (ATT→ACT)	mukE →	chromosome partitioning protein MukE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	976,235	0	T	C	98.7%	284.9 / ‑4.6	78	I205T (ATT→ACT)	mukE	chromosome partitioning protein MukE
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (31/46); total (31/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

AACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGG  >  NC_000913/976099‑976370

aaCCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCg                                                                                                                                      >  2:499956/1‑140 (MQ=255)

  ccGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTg                                                                                                                                    <  1:466911/140‑1 (MQ=255)

  ccGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTg                                                                                                                                    <  1:1191069/140‑1 (MQ=255)

     tCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATg                                                                                                                                 >  1:80146/1‑140 (MQ=255)

     tCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATg                                                                                                                                 >  1:812199/1‑140 (MQ=255)

      cTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATgg                                                                                                                                <  1:1260809/140‑1 (MQ=255)

      cTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATgg                                                                                                                                <  1:1445164/140‑1 (MQ=255)

      cTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATgg                                                                                                                                <  1:386897/140‑1 (MQ=255)

       tGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGc                                                                                                                               <  1:355130/140‑1 (MQ=255)

        gCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATggtg                                                                                                                              <  1:1463615/140‑3 (MQ=255)

                tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATg                                                                                                                      >  2:895199/1‑140 (MQ=255)

                tAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATg                                                                                                                      >  1:399429/1‑140 (MQ=255)

                 aGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGc                                                                                                                     <  2:762083/140‑1 (MQ=255)

                 aGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGc                                                                                                                     >  1:469216/1‑140 (MQ=255)

                      tGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGAtt                                                                                                                >  1:259593/1‑140 (MQ=255)

                       ggTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTg                                                                                                               >  1:689269/1‑140 (MQ=255)

                               ttATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAatcat                                                                                                       <  2:1161754/140‑1 (MQ=255)

                                  tGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTg                                                                                                    >  2:1054624/1‑140 (MQ=255)

                                  tGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTg                                                                                                    <  2:998195/140‑1 (MQ=255)

                                   gggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGc                                                                                                   >  1:385308/1‑140 (MQ=255)

                                   gggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGc                                                                                                   >  1:686095/1‑140 (MQ=255)

                                   gggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGc                                                                                                   >  2:1036054/1‑140 (MQ=255)

                                   gggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGAACCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCAGCTGc                                                                                                   >  2:718223/1‑140 (MQ=255)

                                    ggCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCa                                                                                                  <  2:698362/140‑1 (MQ=255)

                                      ccACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAAc                                                                                                >  1:921429/1‑140 (MQ=255)

                                        aCGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTc                                                                                              >  2:914496/1‑140 (MQ=255)

                                        aCGACAGCAGCAAGTTTCGCATTACCGAATCGGAGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTTAAGCACAGCGTCGCCTGACTCGTGATGGCGCAGCAATGCCGATTGAAAATCATCTGCAACTc                                                                                              >  2:663299/1‑140 (MQ=255)

                                               cagcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATg                                                                                       >  2:376022/1‑140 (MQ=255)

                                               cagcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATg                                                                                       <  2:1116578/140‑1 (MQ=255)

                                               cagcaAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATg                                                                                       <  1:747527/140‑1 (MQ=255)

                                                   aaGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAc                                                                                   <  1:229299/140‑1 (MQ=255)

                                                    aGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAAcc                                                                                  >  1:118023/1‑140 (MQ=255)

                                                       ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGaa                                                                               <  2:337434/140‑1 (MQ=255)

                                                       ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGaa                                                                               <  2:39729/140‑1 (MQ=255)

                                                       ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGaa                                                                               <  1:895199/140‑1 (MQ=255)

                                                       ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGaa                                                                               <  2:788201/140‑1 (MQ=255)

                                                       ttCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGaa                                                                               <  1:486248/140‑1 (MQ=255)

                                                             ttAGCGAATCGGTGTTCCGCCGCGGGGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCCCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAAt                                                                         <  2:1541132/140‑1 (MQ=255)

                                                             ttACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAAt                                                                         <  2:408957/140‑1 (MQ=255)

                                                               aCCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCa                                                                       >  2:521745/1‑140 (MQ=255)

                                                                 cGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGc                                                                     >  2:492298/1‑140 (MQ=255)

                                                                  gAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGcc                                                                    <  1:201417/140‑1 (MQ=255)

                                                                  gAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGcc                                                                    <  1:1036054/140‑1 (MQ=255)

                                                                     tCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGa                                                                 <  2:686095/140‑1 (MQ=255)

                                                                        gtgtTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAg                                                              >  1:786191/1‑140 (MQ=255)

                                                                        gtgtTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAg                                                              <  1:718223/140‑1 (MQ=255)

                                                                        gtgtTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAg                                                              <  2:399429/140‑1 (MQ=255)

                                                                          gtTGCGCGTCGGAGCCGATGTGCGTGCTGGCGAGGGTCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGGAGCAATGCCGATTGAAAATCATCTGCAACTCAACGAAGAAACCGAAGAGAATCAGCCAGATAGCg                                                            <  2:340467/140‑1 (MQ=255)

                                                                          gtTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCCACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCg                                                            >  2:1420607/1‑140 (MQ=255)

                                                                          gtTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCg                                                            <  1:313864/140‑1 (MQ=255)

                                                                             ccGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGgag                                                         >  2:637985/1‑140 (MQ=255)

                                                                             ccGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGgag                                                         <  2:1094302/140‑1 (MQ=255)

                                                                               gCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCGCAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAACATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGagag                                                       <  2:235376/140‑1 (MQ=255)

                                                                               gCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGagag                                                       <  1:1054624/140‑1 (MQ=255)

                                                                                cTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAgg                                                      <  2:689269/140‑1 (MQ=255)

                                                                                cTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAgg                                                      <  1:825491/140‑1 (MQ=255)

                                                                                cTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAgg                                                      <  2:1155490/140‑1 (MQ=255)

                                                                                cTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAgg                                                      <  1:1152814/140‑1 (MQ=255)

                                                                                 ttCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGga                                                     <  1:306558/140‑1 (MQ=255)

                                                                                   cGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaag                                                   >  2:679829/1‑140 (MQ=255)

                                                                                   cGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaag                                                   >  1:881695/1‑140 (MQ=255)

                                                                                     gAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGgaagaa                                                 <  1:656174/140‑1 (MQ=255)

                                                                                      ggCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGaat                                                <  2:600331/139‑1 (MQ=255)

                                                                                           aTGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGa                                           >  2:317239/1‑140 (MQ=255)

                                                                                               gcgtgcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGa                                       <  2:1236312/140‑1 (MQ=255)

                                                                                                cgtgcTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGaa                                      <  1:1545322/140‑1 (MQ=255)

                                                                                                     tGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAACAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCgg                                 >  1:1199464/1‑140 (MQ=255)

                                                                                                                  cGTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCa                    <  1:637985/140‑1 (MQ=255)

                                                                                                                   gTGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCAc                   >  2:545050/1‑140 (MQ=255)

                                                                                                                    tGAAGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACt                  <  1:467520/140‑1 (MQ=255)

                                                                                                                      aaGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGa                <  1:679747/140‑1 (MQ=255)

                                                                                                                       aGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGAc               <  2:469216/140‑1 (MQ=255)

                                                                                                                       aGCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGAc               <  1:679829/140‑1 (MQ=255)

                                                                                                                        gCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTTGCTCACTGACg              <  2:259593/140‑1 (MQ=255)

                                                                                                                        gCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACg              <  2:6537/140‑1 (MQ=255)

                                                                                                                        gCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACg              >  2:420868/1‑140 (MQ=255)

                                                                                                                        gCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACg              <  2:812199/140‑1 (MQ=255)

                                                                                                                        gCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACg              <  1:499956/140‑1 (MQ=255)

                                                                                                                        gCACAGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACg              >  2:928004/1‑140 (MQ=255)

                                                                                                                         cacaGCGTCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGc             >  2:1467495/1‑140 (MQ=255)

                                                                                                                           caacatCGCCTGTTTCGAGATTGTTAATCAATGGCGATTTAAAAGCTTCTGCAAACTAACCAAGAATTCGAAGGGGATCTGCCTGATGTCGGATAGGAAAAAATATGGTTTAACGCGGTAAATTTCGCTCACTGACGTTg           <  2:885384/135‑1 (MQ=255)

                                                                                                                                tCGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTaa      >  2:1128536/1‑140 (MQ=255)

                                                                                                                                 cGCCTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAAc     >  1:79272/1‑140 (MQ=255)

                                                                                                                                    cTGACTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTgg  <  1:988942/140‑1 (MQ=255)

AACCGTCTGCGTCGTTTAGGCATGGTGTGGTTTATGGGCCACGACAGCAGCAAGTTTCGCATTACCGAATCGGTGTTCCGCTTCGGAGCCGATGTGCGTGCTGGCGACGATCCCCGTGAAGCACAGCGTCGCCTGATTCGTGATGGCGAAGCAATGCCGATTGAAAATCATCTGCAACTCAACGATGAAACCGAAGAGAATCAGCCAGATAGCGGAGAGGAAGAATAATGATTGAACGCGGTAAATTTCGCTCACTGACGCTGATTAACTGG  >  NC_000913/976099‑976370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A