Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,895	(A)_7→8	intergenic (‑92/+33)	dauA ← / ← prs	aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,888	1	.	A	85.5%	163.6 / 20.5	55	intergenic (‑85/+40)	dauA/prs	aerobic C4‑dicarboxylate transporter DauA/ribose‑phosphate diphosphokinase
Reads supporting (aligned to +/- strand): ref base . (0/8); new base A (39/8); total (39/16)
Fisher's exact test for biased strand distribution p-value = 1.06e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCG  >  NC_000913/1260753‑1261022

gTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGCCATACAAATGGATATTAC‑AG‑AC‑aaaa                                                                                                                                      >  1:1365929/1‑140 (MQ=255)

   gaacAGAGCGCGGAAAGGCATCCAAGGTGAGAAAAATATTTTTTCCACAATAATGTTTCATCCGGGGGCGAATCATCTGCCAACAAAAGGCCGGGTGAGGGGGGCAGGGGTCATAAAAATGGATATTAC‑AG‑AC‑aaaaaac                                                                                                                                   <  2:1281503/137‑2 (MQ=255)

   gATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑AC‑aaaaaa‑a                                                                                                                                  >  1:113183/1‑140 (MQ=255)

          gcgcGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑Cccgcc                                                                                                                           >  1:113981/1‑140 (MQ=255)

           cgcgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑Cccgccg                                                                                                                          >  1:1013085/1‑140 (MQ=255)

           cgcgGAAAGGCATCACATGGGAGAAAAATATTTTGTTCACAATAATGTTTCACCCGGGAGCGCATCATCTGCAAACAAAAGGGCAGGTGAGGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑A‑ccccgccg                                                                                                                         <  2:612157/140‑6 (MQ=255)

             gggAAAGGCATCACATGTGAGGAAAATTTTTTGTCCACAATAATGTTTCATCGGGGGGCGCATCATCTGTCAACAAAAGGGCGGGTGAAGGGGGCATGGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCa                                                                                                                        <  2:1036541/139‑1 (MQ=255)

             cgGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCa                                                                                                                        >  2:62077/1‑140 (MQ=255)

              ggAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAGGGAGGCATGGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAg                                                                                                                       <  1:690017/140‑1 (MQ=255)

                aaaGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCg                                                                                                                     >  2:977395/1‑140 (MQ=255)

                    gCATCACAGGTGAGAAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACAAAATGGCGGGGGAGGGAGGCATAGGTCATAAAAAGGGATATTAC‑AGAAA‑AAAAAA‑A‑CCCGCCGCAGCGGGTc                                                                                                                 <  2:1535615/140‑1 (MQ=255)

                     cATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCt                                                                                                                >  1:142403/1‑140 (MQ=255)

                             gtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCg                                                                                                        >  1:652567/1‑140 (MQ=255)

                             gtgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATAAAAATGGATATTAC‑AGAAA‑AAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCg                                                                                                        <  1:1406991/140‑1 (MQ=255)

                              tgAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCgg                                                                                                       >  2:4339/1‑140 (MQ=255)

                                     aaTATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAt                                                                                                <  1:179000/140‑1 (MQ=255)

                                      attttttGTTCAAAAAAGTGTTTCGGCGGTGAGCCCATCGTCTGTCAACAAAATGGCGGGAGAAGGGGCAATAGGTCATAAAAATGGATATTAC‑AGAAA‑AAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGAtt                                                                                               <  2:765663/137‑1 (MQ=255)

                                       ttttttGTTCACAATATTGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCGGGGGAAGGGGGCATGGGTCATAAAATTGGATATTAC‑AG‑AA‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                                              <  2:965135/138‑1 (MQ=255)

                                       ttttttGTTCAAAATAATATTCGAACCGTGGGCCACTCATCTGTCAATGAAAAGGCGGGGGAGGGCGGAATTGGTAAAAGAAATGAATTTTACGAA‑AA‑AAAAAA‑C‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTa                                                                                              <  2:171171/138‑1 (MQ=255)

                                             gTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTc                                                                                        <  1:1215972/140‑1 (MQ=255)

                                                cacaATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑AA‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGaa                                                                                     <  1:110675/140‑1 (MQ=255)

                                                        tGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCag                                                                             >  2:737548/1‑140 (MQ=255)

                                                        tGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCCGGTTCGATTAGTGTTCGAACATGGCag                                                                             >  2:235075/1‑140 (MQ=255)

                                                          tttCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCagag                                                                           >  1:195951/1‑140 (MQ=255)

                                                             cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                        >  1:295590/1‑140 (MQ=255)

                                                             cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                        >  2:1457538/1‑140 (MQ=255)

                                                             cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                        >  1:600450/1‑140 (MQ=255)

                                                             cATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATc                                                                        >  1:670619/1‑140 (MQ=255)

                                                               tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATTCAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                      >  1:1367594/1‑140 (MQ=255)

                                                               tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                      >  2:596418/1‑140 (MQ=255)

                                                               tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                      >  1:1048969/1‑140 (MQ=255)

                                                               tCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGa                                                                      >  1:379387/1‑140 (MQ=255)

                                                                 cGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGGGGCATAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                    <  2:1142689/140‑1 (MQ=255)

                                                                 cGTGAGCGCATCATCTGTCAACTAAAGGGCAGGGGAGGGAGGCATGGGTCATACAAAGGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAtt                                                                    <  2:1417672/140‑1 (MQ=255)

                                                                   tGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttct                                                                  >  1:1058210/1‑140 (MQ=255)

                                                                    gAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttctt                                                                 >  2:1317603/1‑140 (MQ=255)

                                                                     ggggcATCATCTGTAAACTAAAGGGAAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGAttcttc                                                                <  2:717994/137‑1 (MQ=255)

                                                                       cgcATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGt                                                              >  1:151423/1‑140 (MQ=255)

                                                                          atcatcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGc                                                           >  2:1173209/1‑140 (MQ=255)

                                                                           tcatcTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGACTCTTCGTTGCt                                                          >  1:956156/1‑140 (MQ=255)

                                                                               cTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATa                                                      >  1:148571/1‑140 (MQ=255)

                                                                                tGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATac                                                     >  1:110654/1‑140 (MQ=255)

                                                                                      cTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGaa                                               >  1:457670/1‑140 (MQ=255)

                                                                                            ggCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt                                         >  1:1483873/1‑140 (MQ=255)

                                                                                            ggCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCtt                                         >  1:19642/1‑140 (MQ=255)

                                                                                               gggTGAAGGAGGAATAGGTAAAAAAAATGGATATTAC‑AG‑AA‑AAAAAACC‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCgg                                      <  1:977395/139‑1 (MQ=255)

                                                                                                  tGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCa                                   <  2:110654/140‑1 (MQ=255)

                                                                                                      ggaggCATGGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAt                               <  1:440281/140‑1 (MQ=255)

                                                                                                        ggtcATAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAc                             <  1:919363/137‑1 (MQ=255)

                                                                                                        gggCATAGGCCATACAAAGGGATATAAA‑AG‑AC‑AAAAAA‑ATCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCAttc                             <  2:856559/139‑3 (MQ=255)

                                                                                                        aggCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATAc                             <  1:737548/140‑1 (MQ=255)

                                                                                                          gCATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCt                           <  1:1384982/140‑1 (MQ=255)

                                                                                                           cATAGGTCATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTg                          >  2:723499/1‑140 (MQ=255)

                                                                                                            aTAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa                         <  2:113183/140‑1 (MQ=255)

                                                                                                            aTAGGTAAACCAACTGAATATTAC‑AA‑CC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGa                         <  2:113981/140‑1 (MQ=255)

                                                                                                                  cATACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                   <  1:1317603/140‑1 (MQ=255)

                                                                                                                  cATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                   <  2:1303953/140‑1 (MQ=255)

                                                                                                                  cATACAAATGGATATTAA‑AG‑AA‑AAAAAA‑ACCCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                   <  2:195951/140‑1 (MQ=255)

                                                                                                                  cATAAAAATGAATATTAC‑AGAAA‑AAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGt                   <  2:670619/140‑1 (MQ=255)

                                                                                                                   aTACAAATGGATATTAC‑AGAAA‑AAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTc                  <  2:379387/140‑1 (MQ=255)

                                                                                                                    tACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                 >  2:1364570/1‑140 (MQ=255)

                                                                                                                    tACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                 >  1:1400463/1‑140 (MQ=255)

                                                                                                                    tACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                 >  2:1163617/1‑140 (MQ=255)

                                                                                                                    tACAAATGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCa                 >  2:1146802/1‑140 (MQ=255)

                                                                                                                         aTGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTa            >  2:907663/1‑140 (MQ=255)

                                                                                                                          tGGATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTAc           >  1:768470/1‑140 (MQ=255)

                                                                                                                            gATATTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGc         >  1:302029/1‑140 (MQ=255)

                                                                                                                              tatTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCAc       >  2:877845/1‑140 (MQ=255)

                                                                                                                               atTAC‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACg      >  1:434669/1‑140 (MQ=255)

                                                                                                                                   c‑AG‑ACAAAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCg  >  2:351564/1‑140 (MQ=255)

GTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTCATCCGTGAGCGCATCATCTGTCAACTAAATGGCAGGTGAAGGAGGCATAGGTCATACAAATGGATATTAC‑AG‑AC‑AAAAAA‑A‑CCCGCCGCAGCGGGTCTTTGAGCCGGGTTCGATTAGTGTTCGAACATGGCAGAGATCGATTCTTCGTTGCTGATACGACGAATCGCTTCGGCCAGCATACCTGACAGGGTCAGAGTACGCACGTTCG  >  NC_000913/1260753‑1261022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A