Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	162	93.0	3098984	99.0%	3067994	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,570,413	A→G	V26A (GTA→GCA)	gadC ←	L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,570,413	0	A	G	98.8%	302.4 / ‑3.4	81	coding (77/1536 nt)	gadC	L‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (38/42); minor base . (1/0); total (39/42)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

TCCCAGCCGTCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATT  >  NC_000913/1570278‑1570549

tcCCAGCCGTCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCAt                                                                                                                                      >  1:855870/1‑140 (MQ=255)

 cccAGCCGTCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATc                                                                                                                                     <  1:1253119/140‑1 (MQ=255)

  ccAGCCGTCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCg                                                                                                                                    >  1:414252/1‑140 (MQ=255)

   cAGCCGTCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGa                                                                                                                                   <  1:937431/140‑1 (MQ=255)

         tCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGt                                                                                                                             >  2:17853/1‑140 (MQ=255)

         tCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGt                                                                                                                             >  1:1268912/1‑140 (MQ=255)

         tCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGt                                                                                                                             >  2:791542/1‑140 (MQ=255)

                tGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCa                                                                                                                      <  1:1164787/140‑1 (MQ=255)

                tGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCa                                                                                                                      >  2:1082211/1‑140 (MQ=255)

                tGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCa                                                                                                                      >  2:403320/1‑140 (MQ=255)

                  gCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCaaa                                                                                                                    <  2:53021/140‑1 (MQ=255)

                     aTTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGaa                                                                                                                 <  1:791513/140‑1 (MQ=255)

                     aTTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGaa                                                                                                                 <  2:1540721/140‑1 (MQ=255)

                       ttCCGCAGCACAAAGTTCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATc                                                                                                               >  2:1107315/1‑140 (MQ=255)

                       ttCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATc                                                                                                               >  1:684336/1‑140 (MQ=255)

                          cGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATAAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCaa                                                                                                            <  1:82054/140‑1 (MQ=255)

                               cacaAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAAt                                                                                                       <  1:107291/140‑1 (MQ=255)

                                  aaaGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAAtgtg                                                                                                    <  1:172336/140‑1 (MQ=255)

                                  aaaGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCAATGCCGTTATGGCAAAGAATCCAAGTAAtgtg                                                                                                    <  2:372381/140‑1 (MQ=255)

                                   aaGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGa                                                                                                   <  1:291940/140‑1 (MQ=255)

                                   aaGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGa                                                                                                   <  2:441710/140‑1 (MQ=255)

                                   aaGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGa                                                                                                   <  1:36773/140‑1 (MQ=255)

                                   aaGTCCCACGGGAACAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGa                                                                                                   <  2:838465/140‑1 (MQ=255)

                                    aGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAg                                                                                                  <  1:229144/140‑1 (MQ=255)

                                    aGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAg                                                                                                  <  2:364414/140‑1 (MQ=255)

                                    aGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAg                                                                                                  <  1:108812/140‑1 (MQ=255)

                                         cACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAgctgct                                                                                             <  2:453720/140‑1 (MQ=255)

                                         cACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAgctgct                                                                                             >  1:430777/1‑140 (MQ=255)

                                                 tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTa                                                                                     <  1:1547681/140‑1 (MQ=255)

                                                 tAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTa                                                                                     <  2:463471/140‑1 (MQ=255)

                                                 aaaaCCATAAAATCCCGCCTAATCGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTa                                                                                     <  1:992975/139‑1 (MQ=255)

                                                  aaaCCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTAc                                                                                    <  1:672078/140‑1 (MQ=255)

                                                    aCCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACct                                                                                  >  2:912790/1‑140 (MQ=255)

                                                      cATAAAATCCCGCCTAATAGCAGTAAGAATACTTATGAAAATCCCGATGTTGCTAAGGTAGGATATCCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACctgt                                                                                <  2:1113051/140‑1 (MQ=255)

                                                      cATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCGTTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACctgt                                                                                <  2:285278/140‑1 (MQ=255)

                                                        tAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACctgtct                                                                              >  1:391631/1‑140 (MQ=255)

                                                         aaaaTCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACctgtctg                                                                             <  2:855870/140‑1 (MQ=255)

                                                                cGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTAc                                                                                    <  1:502173/126‑1 (MQ=255)

                                                                cGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTAc                                                                                    >  2:502173/1‑126 (MQ=255)

                                                                cGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGAt                                                                      >  1:213633/1‑140 (MQ=255)

                                                                  ccTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCCCAGGTAGGGTAGTCATAAACAGCCATTGCCATCGATGCCGGTATGGCATTTAGTCAAAGAATTGCGCCCTGCATATCTTTACCTGTCAGTACTGATGt                                                                    >  2:1464514/1‑140 (MQ=255)

                                                                   cTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTa                                                                   <  1:252809/140‑1 (MQ=255)

                                                                    tAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAGGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAg                                                                  <  2:414252/140‑1 (MQ=255)

                                                                      aTAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGcc                                                                <  2:1268912/140‑1 (MQ=255)

                                                                           aGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCACCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtatt                                                           <  2:432212/140‑1 (MQ=255)

                                                                            ggAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCAtatta                                                          >  2:1236744/1‑140 (MQ=255)

                                                                                   gaCTAATGGAAAGCCCGACGTTGCTATAATACGGTAATCATAAACACCAATTGCCATCGATGTCGTTATGGACAAGAATCCATGTAATGCGTGCTGGTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCt                                                   >  2:296257/1‑140 (MQ=255)

                                                                                   gaCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCt                                                   >  2:533368/1‑140 (MQ=255)

                                                                                    aCTAATGAAAAGCCCTATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTa                                                  >  2:886088/1‑140 (MQ=255)

                                                                                    aCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCTTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTa                                                  <  1:367208/140‑1 (MQ=255)

                                                                                    aCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTa                                                  <  1:403320/140‑1 (MQ=255)

                                                                                    aCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTa                                                  >  2:570272/1‑140 (MQ=255)

                                                                                    aCTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTa                                                  >  1:273589/1‑140 (MQ=255)

                                                                                     cTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTaa                                                 <  2:430777/140‑1 (MQ=255)

                                                                                         tGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACg                                             >  1:1506500/1‑140 (MQ=255)

                                                                                                cccGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTcc                                      >  2:841322/1‑140 (MQ=255)

                                                                                                cccGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTcc                                      >  2:708532/1‑140 (MQ=255)

                                                                                                  cGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTg                                    >  1:913357/1‑140 (MQ=255)

                                                                                                   gATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGt                                   >  1:807390/1‑140 (MQ=255)

                                                                                                         gCGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAc                             <  2:684336/140‑1 (MQ=255)

                                                                                                          cGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAcc                            >  1:188507/1‑140 (MQ=255)

                                                                                                          cGAAGGTAGGGTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAAcc                            >  1:1056915/1‑140 (MQ=255)

                                                                                                          cGAAGGTAGGGTATTCATAAACAGCCATT‑CCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGGCGGAACCg                           >  1:279397/1‑140 (MQ=255)

                                                                                                                   ggTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGtt                   >  1:948092/1‑140 (MQ=255)

                                                                                                                    gTATTCATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGttt                  >  2:1351660/1‑140 (MQ=255)

                                                                                                                         cATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTc             >  2:12919/1‑140 (MQ=255)

                                                                                                                         cATAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTc             >  1:1208777/1‑140 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  2:412226/140‑1 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  2:1304385/140‑1 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  1:901455/140‑1 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  2:391631/140‑1 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  1:1200621/140‑1 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  1:1236744/140‑1 (MQ=255)

                                                                                                                          aTAAACAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCt            <  1:912790/140‑1 (MQ=255)

                                                                                                                              aCAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAg        <  1:1107315/140‑1 (MQ=255)

                                                                                                                               cAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGc       >  2:550201/1‑140 (MQ=255)

                                                                                                                               cAGCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGc       >  2:488060/1‑140 (MQ=255)

                                                                                                                                 gCCATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGt     >  1:310544/1‑140 (MQ=255)

                                                                                                                                  ccATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa    >  1:391394/1‑140 (MQ=255)

                                                                                                                                  ccATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa    >  2:599602/1‑140 (MQ=255)

                                                                                                                                  ccATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa    >  2:300056/1‑140 (MQ=255)

                                                                                                                                  ccATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTa    >  2:1512709/1‑140 (MQ=255)

                                                                                                                                  ccATTGCCATCGATGCCGTTATGGCAAAGAATCCAAGCAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCAGTTTTGTTCTGAAGCGTa    >  2:1234347/1‑140 (MQ=255)

                                                                                                                                    aTTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt  <  1:1082211/140‑1 (MQ=255)

                                                                                                                                    aTTGCCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTAtt  <  1:17853/140‑1 (MQ=255)

TCCCAGCCGTCGACGGTGGCCATTTCCGCAGCACAAAGTCCCACGGGAATAAACCATAAAATCCCGCCTAATAGCAGGAAGAAGACTAATGAAAAGCCCGATGTTGCGAAGGTAGGGTATTCATAAACAGCCATTACCATCGATGCCGTTATGGCAAAGAATCCAAGTAATGTGAGCTGCTTAGCTTTACCTGTCTGTACTGATGTAGCCATATTATCCCCCTAAAACGGTATTCCTGTCGGAACCGCACCTGTTTTGTTCTGAAGCGTATT  >  NC_000913/1570278‑1570549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A