Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F28 I1 R1 162 93.0 3098984 99.0% 3067994 139.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,714,3180T.98.2% 228.3 / ‑1.7 55intergenic (+47/‑59)dtpA/gstAdipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (22/32);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTAC  >  NC_000913/1714181‑1714453
                                                                                                                                          |                                                                                                                                       
tGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttt                                                                                                                                       >  1:98322/1‑140 (MQ=255)
   aGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttttt                                                                                                                                    >  1:861666/1‑140 (MQ=255)
   aGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCtttttt                                                                                                                                    <  1:568732/140‑1 (MQ=255)
       cTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTATTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgt                                                                                                                                >  1:894911/1‑138 (MQ=255)
         gATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCttttttttgttc                                                                                                                              >  2:221721/1‑136 (MQ=255)
          aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGTCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                                            >  2:716416/1‑140 (MQ=255)
          aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                                            >  2:1416211/1‑140 (MQ=255)
          aTGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCa                                                                                                                            >  1:35630/1‑140 (MQ=255)
           tgctgctgcCCGCGCCGAAACTGCCCCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                                                                           <  2:176718/140‑1 (MQ=255)
           tgctgcTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAg                                                                                                                           <  2:1172111/140‑1 (MQ=255)
            gctgctGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGc                                                                                                                          >  1:640780/1‑140 (MQ=255)
               gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTT‑TTCTGCAAc                                                                                                                       >  2:1014916/1‑140 (MQ=255)
               gctgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAAc                                                                                                                       >  2:1187482/1‑140 (MQ=255)
                ctgCCCGCGCCGAAACTGCCCCGCATGCCGCGGGATGACGCTGCAGACAAGGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACg                                                                                                                      <  2:1087833/140‑1 (MQ=255)
                 tgACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTGGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGc                                                                                                                     <  2:338879/140‑1 (MQ=255)
                  gACTGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                                                                    <  1:611729/140‑1 (MQ=255)
                  gACCGCGCCGAAACTGCCCCGCAGGACGCAGGATGACGCTGCAGACAAAGCGGGGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                                                                    <  2:119158/140‑1 (MQ=255)
                  gACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                                                                    <  1:1187482/140‑1 (MQ=255)
                  gACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                                                                    <  1:157743/140‑1 (MQ=255)
                  gACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCa                                                                                                                    <  1:687214/140‑1 (MQ=255)
                       cgcCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTAcc                                                                                                               <  2:292816/140‑1 (MQ=255)
                       ccccGAACATGCACGGCAGGCCGCAGGGTGACGCTCCAGAGAAAGCGGCGAAA‑CCCGCGTTAGCTTAAAATTCAGGGAAACCCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGTAACGCACTAcc                                                                                                               <  2:429804/138‑1 (MQ=255)
                                  cACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAAc                                                                                                    >  1:1295383/1‑140 (MQ=255)
                                      gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                                <  1:582713/140‑1 (MQ=255)
                                      gCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACcaca                                                                                                <  2:649268/140‑1 (MQ=255)
                                         tggcGCAGGATGGCGCTGGAGAAAAAGGGGGGAAA‑GCAGCCGTGGGGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGAc                                                                                             <  2:958616/137‑1 (MQ=255)
                                               aGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAgg                                                                                       >  2:1266921/1‑140 (MQ=255)
                                                           cAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                                                           >  1:640787/1‑140 (MQ=255)
                                                           cAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                                                           >  2:1521608/1‑140 (MQ=255)
                                                           cAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                                                           >  2:521658/1‑140 (MQ=255)
                                                           aaGACAAAGCGGCAAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGa                                                                           <  1:323854/139‑1 (MQ=255)
                                                                aaaGGGGGGAAAGGCAGCGGTAGCGTAAACTTCAGGAAAACTCTTTTACGAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAAtt                                                                       <  2:365565/140‑1 (MQ=255)
                                                                   gcggcgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                                   <  1:648952/140‑1 (MQ=255)
                                                                   gcggcgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                                   >  1:183714/1‑140 (MQ=255)
                                                                   gcggcgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                                   >  2:148423/1‑140 (MQ=255)
                                                                   gcggcgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                                   <  1:537373/140‑1 (MQ=255)
                                                                   gcggcgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                                   <  1:185996/140‑1 (MQ=255)
                                                                   gcggcgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTc                                                                   >  1:1053124/1‑140 (MQ=255)
                                                                       cgAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACa                                                               >  1:1319618/1‑140 (MQ=255)
                                                                         aaa‑GCAGCCGTAGCGTAAAATTCAGGGAACCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACaaa                                                             <  2:1115423/140‑1 (MQ=255)
                                                                            ggcaccggTAGTGTAAAATTCAGGGAATCTCTTTAGCAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCAGTACCATACCTTAAACCACAGGCAAAAGGAGTTACCGATGAAATTGTTCTACAAAcc                                                           <  2:989579/133‑1 (MQ=255)
                                                                              cagcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCgg                                                         <  2:98322/140‑1 (MQ=255)
                                                                              cagcCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCgg                                                         <  1:50834/140‑1 (MQ=255)
                                                                                 ccGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTg                                                      >  2:1532493/1‑140 (MQ=255)
                                                                                     aGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTg                                                  <  2:861666/140‑1 (MQ=255)
                                                                                        gTAAAATTCGGGGAAACCTTTTAACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACAGATTAAATTGTTCTACAAACCGGGTGCCTGctc                                               <  2:925615/140‑1 (MQ=255)
                                                                                             aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCg                                          <  1:1289758/140‑1 (MQ=255)
                                                                                             aTTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCg                                          <  2:40497/140‑1 (MQ=255)
                                                                                               tCAGGGAATCTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCt                                        <  2:804341/140‑1 (MQ=255)
                                                                                               tCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCt                                        >  1:143751/1‑140 (MQ=255)
                                                                                                 aGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTc                                      <  2:1053124/140‑1 (MQ=255)
                                                                                                 aGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTc                                      <  1:221721/140‑1 (MQ=255)
                                                                                                           ttttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCAcc                            <  1:1400547/140‑1 (MQ=255)
                                                                                                             ttACAAGCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCt                          >  1:1095536/1‑140 (MQ=255)
                                                                                                                   gCCGCTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTga                    >  2:488448/1‑140 (MQ=255)
                                                                                                                       cTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGc                >  1:63841/1‑140 (MQ=255)
                                                                                                                       cTAACTTTTCGTTAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGc                >  2:239280/1‑140 (MQ=255)
                                                                                                                                   tAGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGAtttt    <  2:183714/140‑1 (MQ=255)
                                                                                                                                    aGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTa   >  2:358926/1‑140 (MQ=255)
                                                                                                                                    aGCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTa   >  2:254179/1‑140 (MQ=255)
                                                                                                                                     gCGGC‑TTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTAc  <  1:729861/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                       
TGCAGTACTGATGCTGCTGACCGCGCCGAAACTGCACCGCATGACGCAGGATGACGCTGCAGACAAAGCGGCGAAA‑GCAGCCGTAGCGTAAAATTCAGGGAAACTCTTTTACAAGCCGCTAACTTTTCGTTAGCGGCTTTTTTTTTGTTCAGCAACGCACTACCATACTTTAAACCACAGACAAAAGGAGTTACCGATGAAATTGTTCTACAAACCGGGTGCCTGCTCTCTCGCTTCCCATATCACCCTGCGTGAGAGCGGAAAGGATTTTAC  >  NC_000913/1714181‑1714453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A