Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 72.5 2472046 99.4% 2457213 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,140,782 A→G L250L (TTA→TTG)  flgL → flagellar hook‑filament junction protein 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,140,7820AG100.0% 251.1 / NA 68L250L (TTA→TTG) flgLflagellar hook‑filament junction protein 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACG  >  NC_000913/1140650‑1140917
                                                                                                                                    |                                                                                                                                       
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  2:976227/1‑140 (MQ=255)
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  1:592710/1‑140 (MQ=255)
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  2:90005/1‑140 (MQ=255)
tGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGc                                                                                                                                  >  1:305110/1‑140 (MQ=255)
  ccATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAg                                                                                                                                >  2:464515/1‑140 (MQ=255)
  ccATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAg                                                                                                                                >  1:187543/1‑140 (MQ=255)
     tCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTg                                                                                                                             >  2:1077860/1‑140 (MQ=255)
     tCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTg                                                                                                                             >  1:229249/1‑140 (MQ=255)
     tCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTg                                                                                                                             >  2:197707/1‑140 (MQ=255)
          gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacga                                                                                                                        >  2:433287/1‑140 (MQ=255)
          gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacga                                                                                                                        >  2:724063/1‑140 (MQ=255)
          gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacga                                                                                                                        >  1:399211/1‑140 (MQ=255)
          gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaa                                                                                                                           <  2:829318/137‑1 (MQ=255)
          gCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaa                                                                                                                           >  1:829318/1‑137 (MQ=255)
           cccTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTgaacgaa                                                                                                                       >  1:761185/1‑140 (MQ=255)
             cTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACt                                                                                                                     >  2:435873/1‑140 (MQ=255)
               gAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTgg                                                                                                                   <  1:976227/140‑1 (MQ=255)
                 aaaCGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAg                                                                                                                 <  1:464515/140‑1 (MQ=255)
                     gCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGc                                                                                                             >  2:959667/1‑140 (MQ=255)
                     gCCGGTCCCGGATAGCGAAGCGGATAAAGAAACCGCCGCCGCCGCGCTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGCATTGGGCACGCAGCTGAACGAACTGGAGCCGc                                                                                                             >  2:742264/1‑140 (MQ=255)
                       cGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTg                                                                                                           <  1:372536/140‑1 (MQ=255)
                        ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTTGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  1:1077860/140‑1 (MQ=255)
                        ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  2:187543/140‑1 (MQ=255)
                        ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  1:1111464/140‑1 (MQ=255)
                        ggTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTgg                                                                                                          <  2:788910/140‑1 (MQ=255)
                          tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGAt                                                                                                        <  1:79498/140‑1 (MQ=255)
                          tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGAt                                                                                                        <  1:1092775/140‑1 (MQ=255)
                          tCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGAt                                                                                                        <  1:197707/140‑1 (MQ=255)
                             cgGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCa                                                                                                     <  2:553268/140‑1 (MQ=255)
                                   gCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGt                                                                                               <  2:399211/140‑1 (MQ=255)
                                         cGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGAt                                                                                         >  1:115378/1‑140 (MQ=255)
                                           gATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATcg                                                                                       <  2:761185/140‑1 (MQ=255)
                                            aTAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATcgc                                                                                      >  1:1017667/1‑140 (MQ=255)
                                                   aaCCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAg                                                                               >  1:35658/1‑140 (MQ=255)
                                                      cgccgcTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGc                                                                            >  1:659709/1‑140 (MQ=255)
                                                              cggcgTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcag                                                                    <  2:605551/140‑1 (MQ=255)
                                                                gcgTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcagca                                                                  >  2:848687/1‑140 (MQ=255)
                                                                   ttAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcag                                                                    <  1:443713/135‑1 (MQ=255)
                                                                   ttAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACgcag                                                                    >  2:443713/1‑135 (MQ=255)
                                                                   ttAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGAt                                                               <  1:175607/140‑1 (MQ=255)
                                                                      gATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                            <  2:229249/140‑1 (MQ=255)
                                                                      gATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                            <  1:532305/140‑1 (MQ=255)
                                                                      gATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAg                                                            <  2:524116/140‑1 (MQ=255)
                                                                         aaaaCCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGa                                                         <  1:435873/140‑1 (MQ=255)
                                                                         aaaaCCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGa                                                         <  2:882079/140‑1 (MQ=255)
                                                                         aaaaCCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGa                                                         <  1:959667/140‑1 (MQ=255)
                                                                           aaccaaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATc                                                       >  2:508450/1‑140 (MQ=255)
                                                                              caaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTgg                                                    <  2:659709/140‑1 (MQ=255)
                                                                               aaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGt                                                   <  2:1017667/140‑1 (MQ=255)
                                                                               aaccGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGt                                                   <  1:90005/140‑1 (MQ=255)
                                                                                accGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGtt                                                  >  2:444564/1‑140 (MQ=255)
                                                                                accGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATGCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGtt                                                  >  2:1074850/1‑140 (MQ=255)
                                                                                   gcgGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGAt                                               <  1:848687/140‑1 (MQ=255)
                                                                                        cTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGa                                          <  1:1074850/140‑1 (MQ=255)
                                                                                           aaaaaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTg                                       >  1:878514/1‑140 (MQ=255)
                                                                                            aaaaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTgg                                      >  2:484351/1‑140 (MQ=255)
                                                                                              aaCTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGaa                                    <  2:35658/140‑1 (MQ=255)
                                                                                                     tGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCaa                               <  2:769362/138‑1 (MQ=255)
                                                                                                     tGAACAATGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCaa                               >  1:769362/1‑138 (MQ=255)
                                                                                                           aTGTGCTGACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCa                       >  1:543561/1‑140 (MQ=255)
                                                                                                                  gACTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAca                <  1:508450/140‑1 (MQ=255)
                                                                                                                   aCTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcat               >  2:783054/1‑140 (MQ=255)
                                                                                                                   aCTGTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcat               >  2:418159/1‑140 (MQ=255)
                                                                                                                      gTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTAcatcat            >  2:548836/1‑140 (MQ=255)
                                                                                                                      gTTCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGAT‑‑GCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgc          >  1:666554/1‑140 (MQ=255)
                                                                                                                       ttCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATg           >  1:1184623/1‑140 (MQ=255)
                                                                                                                        tCGCGCGGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgc          >  2:534504/1‑140 (MQ=255)
                                                                                                                         cgcgcgGAATTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATgca         <  2:827325/140‑1 (MQ=255)
                                                                                                                                aaTTGGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACg  <  2:115378/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                       
TGCCATCGCAGCCCTGAAAACGCCGGTCGCGGATAGCGAAGCGGATAAAGAAACCGCCGCTGCGGCGTTAGATAAAACCAACCGCGGACTGAAAAACTCGCTGAACAATGTGCTGACTGTTCGCGCGGAATTAGGCACGCAGCTGAACGAACTGGAGTCGCTGGATTCATTAGGTAGCGATCGCGCTTTAGGGCAAACGCAGCAGATGAGCGATCTGGTTGATGTGGACTGGAATGCAACTATTTCATCTTACATCATGCAGCAAACG  >  NC_000913/1140650‑1140917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A