Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,292,624	A→G	V100A (GTT→GCT)	hns ←	DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,292,624	0	A	G	100.0%	127.7 / NA	35	V100A (GTT→GCT)	hns	DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/13); total (22/13)

AGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGC  >  NC_000913/1292491‑1292749

aGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCg                                                                                                                             <  2:700951/136‑1 (MQ=255)

aGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCg                                                                                                                             >  1:700951/1‑136 (MQ=255)

 gTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCt                                                                                                                        >  2:694509/1‑140 (MQ=255)

            aaaaGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCg                                                                                                                             >  2:413181/1‑124 (MQ=255)

            aaaaGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCg                                                                                                             <  1:516020/140‑1 (MQ=255)

              aaGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGa                                                                                                           >  2:93805/1‑140 (MQ=255)

                   tattGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTg                                                                                                      >  2:744498/1‑140 (MQ=255)

                   tattGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGC‑TTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGa                                                                                                     >  1:47920/1‑140 (MQ=255)

                               aGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGc                                                                                          >  1:1188609/1‑140 (MQ=255)

                                 gAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTCGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCtt                                                                                        >  1:882748/1‑140 (MQ=255)

                                      cgtcgAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTg                                                                                   >  1:295892/1‑140 (MQ=255)

                                           aGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGa                                                                              >  1:727888/1‑140 (MQ=255)

                                                        gCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGgcagca                                                                 >  1:790963/1‑140 (MQ=255)

                                                         cTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCaa                                                                >  1:797306/1‑140 (MQ=255)

                                                              ccATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTa                                                           >  1:155141/1‑140 (MQ=255)

                                                                 ttGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTc                                                        >  2:356149/1‑140 (MQ=255)

                                                                  tGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTca                                                       >  1:1009414/1‑140 (MQ=255)

                                                                   gCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTcag                                                      >  2:905825/1‑140 (MQ=255)

                                                                            aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  2:155141/140‑1 (MQ=255)

                                                                            aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  2:1188609/140‑1 (MQ=255)

                                                                            aTTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  1:905825/140‑1 (MQ=255)

                                                                                     ggAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAt                                    <  2:47920/140‑1 (MQ=255)

                                                                                     cgAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAAt                                    <  2:882748/139‑1 (MQ=255)

                                                                                       aGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             <  1:931935/129‑1 (MQ=255)

                                                                                       aGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAgttcgtt                                             >  2:931935/1‑129 (MQ=255)

                                                                                       aGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATAc                                  <  2:936845/140‑1 (MQ=255)

                                                                                                ttGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGa                         >  2:33807/1‑140 (MQ=255)

                                                                                                            ggTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTcgc             <  2:797306/140‑1 (MQ=255)

                                                                                                             gTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAATCAGCAGTTCGTTCGGGTCAATACCGTCa                             >  1:439463/1‑123 (MQ=255)

                                                                                                             gTTTTAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTAATCAGCAGTTCGTTCGGGTCAATACCGTCa                             <  2:439463/123‑1 (MQ=255)

                                                                                                              ttttAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGa           <  2:295892/140‑1 (MQ=255)

                                                                                                              ttttAGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGa           <  1:33807/140‑1 (MQ=255)

                                                                                                                  aGTTTCGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATAtt       >  2:175764/1‑140 (MQ=255)

                                                                                                                       cGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtgctgc  >  2:51349/1‑140 (MQ=255)

                                                                                                                       cGCCGTTTTCGTCAGCGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATtgctgc  >  1:895492/1‑140 (MQ=255)

AGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTGAGCACGTTTAGCTTTGGTGCCAGATTTAACGGCAGCAAGGCTATTCAGCAGTTCGTTCGGGTCAATACCGTCAGCGATCAGCATTTCGCGATATTGCTGC  >  NC_000913/1292491‑1292749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A