Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,335,827	A→G	intergenic (+160/‑4)	ymiC → / → acnA	protein YmiC/aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,335,827	0	A	G	100.0%	222.2 / NA	60	intergenic (+160/‑4)	ymiC/acnA	protein YmiC/aconitate hydratase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/34); total (26/34)

TTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGT  >  NC_000913/1335691‑1335951

ttGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCt                                                                                                                           <  2:254953/140‑1 (MQ=255)

  gTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTAt                                                                                                                         >  1:65743/1‑140 (MQ=255)

        tgctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATgtcgtc                                                                                                                   <  2:577555/140‑1 (MQ=255)

        tgctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATgtcgtc                                                                                                                   >  1:342414/1‑140 (MQ=255)

          ctttgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCaa                                                                                                                 >  1:758022/1‑140 (MQ=255)

             tgcCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAAccc                                                                                                              <  2:758022/140‑1 (MQ=255)

                 acaAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACg                                                                                                          <  2:1024857/140‑1 (MQ=255)

                  caAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACga                                                                                                         <  1:1122997/140‑1 (MQ=255)

                           tcctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGt                                                                                                <  1:1060647/140‑1 (MQ=255)

                           tcctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGt                                                                                                <  2:342414/140‑1 (MQ=255)

                            cctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTa                                                                                               <  2:924688/140‑1 (MQ=255)

                            cctcTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTa                                                                                               <  2:65743/140‑1 (MQ=255)

                               ctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAgg                                                                                            >  1:210801/1‑140 (MQ=255)

                               ctTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAgg                                                                                            >  2:949271/1‑140 (MQ=255)

                                ttttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGa                                                                                           <  2:844480/140‑1 (MQ=255)

                                  ttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                                                                         <  2:508145/140‑1 (MQ=255)

                                  ttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                                                                         <  2:908645/140‑1 (MQ=255)

                                  ttATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGaca                                                                                         <  2:782098/140‑1 (MQ=255)

                                   tATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGacac                                                                                        >  2:875112/1‑140 (MQ=255)

                                     tCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGt                                                                                      >  2:517402/1‑140 (MQ=255)

                                            gggTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTGAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGc                                                                               <  2:126238/140‑1 (MQ=255)

                                            gggTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGc                                                                               >  1:126238/1‑140 (MQ=255)

                                              gttgttATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCa                                                                             >  1:699955/1‑140 (MQ=255)

                                                  ttATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGa                                                                         <  1:923402/140‑1 (MQ=255)

                                                    aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                       >  2:148112/1‑140 (MQ=255)

                                                    aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                       <  1:285908/140‑1 (MQ=255)

                                                    aTCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATa                                                                       <  2:845624/140‑1 (MQ=255)

                                                          tCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                                 <  1:148112/140‑1 (MQ=255)

                                                          tCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                                 <  2:1128230/140‑1 (MQ=255)

                                                          tCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACtt                                                                 <  1:1195665/140‑1 (MQ=255)

                                                             ttACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc                                                              >  2:712925/1‑140 (MQ=255)

                                                             ttACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAcc                                                              <  1:949271/140‑1 (MQ=255)

                                                                   gATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAc                                                               >  1:807360/1‑133 (MQ=255)

                                                                   gATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTAc                                                               <  2:807360/133‑1 (MQ=255)

                                                                               aTCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGc                                            >  1:356640/1‑140 (MQ=255)

                                                                                tCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCt                                           <  2:562783/140‑1 (MQ=255)

                                                                                  tttAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTtg                                         <  2:745436/140‑1 (MQ=255)

                                                                                          tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCtt                                          >  2:98917/1‑131 (MQ=255)

                                                                                          tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCtt                                          <  1:98917/131‑1 (MQ=255)

                                                                                          tCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTAGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTaaa                                 <  1:1134194/140‑1 (MQ=255)

                                                                                           cACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAAt                                >  2:216709/1‑140 (MQ=255)

                                                                                            aCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATc                               >  1:1202529/1‑140 (MQ=255)

                                                                                            aCCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATc                               >  1:520381/1‑140 (MQ=255)

                                                                                             cccTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                              <  1:606169/140‑1 (MQ=255)

                                                                                                tGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTg                           <  2:815061/140‑1 (MQ=255)

                                                                                                  aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                              >  1:1019146/1‑135 (MQ=255)

                                                                                                  aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCa                              <  2:1019146/135‑1 (MQ=255)

                                                                                                  aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTggg                         <  1:875112/140‑1 (MQ=255)

                                                                                                  aaGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTggg                         >  2:35672/1‑140 (MQ=255)

                                                                                                        aTCAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGatat                   >  1:699391/1‑140 (MQ=255)

                                                                                                          cAGGGCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCa                 <  1:35672/140‑1 (MQ=255)

                                                                                                            gggCTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCAcc               >  2:905505/1‑140 (MQ=255)

                                                                                                               cTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt            <  1:905505/140‑1 (MQ=255)

                                                                                                               cTTCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGt            <  2:699391/140‑1 (MQ=255)

                                                                                                                 tCGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCt          >  1:756238/1‑140 (MQ=255)

                                                                                                                  cGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGa                      >  1:633398/1‑127 (MQ=255)

                                                                                                                  cGCAACCCTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGa                      <  2:633398/127‑1 (MQ=255)

                                                                                                                       cccTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCaa    >  1:966760/1‑140 (MQ=255)

                                                                                                                       cccTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCaa    >  2:1011179/1‑140 (MQ=255)

                                                                                                                         cTGTCATTAAGGAGGGGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGt  >  1:155166/1‑140 (MQ=255)

TTGTCATTTGCTTTGCCACAAGGTTTCTCCTCTTTTATCAATTTGGGTTGTTATCAAATCGTTACGCGATGTTTGTGTTATCTTTAATATTCACCCTGAAGAGAATCAGGGCTTCGCAACCCTGTCATTAAGGAGGAGCTATGTCGTCAACCCTACGAGAAGCCAGTAAGGACACGTTGCAGGCCAAAGATAAAACTTACCACTACTACAGCCTGCCGCTTGCTGCTAAATCACTGGGCGATATCACCCGTCTACCCAAGT  >  NC_000913/1335691‑1335951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A