Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,848,406	T→C	T91A (ACG→GCG)	ydjA ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,848,406	0	T	C	95.2%	140.1 / ‑2.0	42	T91A (ACG→GCG)	ydjA	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (19/21); total (20/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAA  >  NC_000913/1848271‑1848535

aTGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGcgatg                                                                                                                               <  2:330606/140‑1 (MQ=255)

 tGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGcgatga                                                                                                                              >  2:686376/1‑140 (MQ=255)

 tGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGcgatga                                                                                                                              >  2:271208/1‑140 (MQ=255)

     ccACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAg                                                                                                                          >  1:1018262/1‑140 (MQ=255)

     ccACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAg                                                                                                                          <  1:686376/140‑1 (MQ=255)

       aCTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCg                                                                                                                        <  2:141790/140‑1 (MQ=255)

            gcCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTgcg                                                                                                                   <  1:574693/140‑1 (MQ=255)

                gATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGa                                                                                                               >  2:250790/1‑140 (MQ=255)

                 aTGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGaa                                                                                                              <  2:1195321/140‑1 (MQ=255)

                    ccgccAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACgg                                                                                                           <  2:1018262/140‑1 (MQ=255)

                     cgccAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGgc                                                                                                          >  2:638611/1‑140 (MQ=255)

                     cgccAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGgc                                                                                                          >  2:952409/1‑140 (MQ=255)

                               cTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACggg                                                                                                >  1:1218068/1‑140 (MQ=255)

                                 cggaaaCTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCt                                                                                              <  1:266948/136‑1 (MQ=255)

                                       ctgctgCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCg                                                                                        >  1:405215/1‑140 (MQ=255)

                                               aTTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCttt                                                                                <  1:638611/140‑1 (MQ=255)

                                               aTTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCAGAACGGCGCATTACGGGCTTTGTCGATAGCttt                                                                                <  1:765547/140‑1 (MQ=255)

                                                   gCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGtca                                                                            <  2:384401/140‑1 (MQ=255)

                                                         ccATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTa                                                                      >  2:1086485/1‑140 (MQ=255)

                                                             gACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCgg                                                                  >  1:1030626/1‑140 (MQ=255)

                                                                  cgcATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGGTTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGgcagca                                                             >  2:1098926/1‑140 (MQ=255)

                                                                      tCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCg                                                         <  1:447722/140‑1 (MQ=255)

                                                                          gCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGcccc                                                     <  1:952409/140‑1 (MQ=255)

                                                                          gCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGcccc                                                     <  2:1030626/140‑1 (MQ=255)

                                                                          gCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGcccc                                                     <  1:268260/140‑1 (MQ=255)

                                                                          gCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGcccc                                                     <  1:271208/140‑1 (MQ=255)

                                                                           cAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccccc                                                    <  2:1218068/140‑1 (MQ=255)

                                                                           cAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTCCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccccc                                                    <  1:1078193/140‑1 (MQ=255)

                                                                           cAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCACATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccccc                                                    <  1:445696/140‑1 (MQ=255)

                                                                                 tttCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTcc                                              >  2:582451/1‑140 (MQ=255)

                                                                                            cAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCt                                   >  2:867891/1‑140 (MQ=255)

                                                                                            cAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCt                                   >  2:321514/1‑140 (MQ=255)

                                                                                             aGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccccc                                                    >  2:154772/1‑122 (MQ=255)

                                                                                             aGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccccc                                                    <  1:154772/122‑1 (MQ=255)

                                                                                             aGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTa                                          <  2:1222122/132‑1 (MQ=255)

                                                                                             aGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTa                                          >  1:1222122/1‑132 (MQ=255)

                                                                                               cgcgGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGaa                                >  1:857160/1‑140 (MQ=255)

                                                                                                    gACTTTATGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCt                           >  1:590883/1‑140 (MQ=255)

                                                                                                           tGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGccc                    <  2:590883/140‑1 (MQ=255)

                                                                                                           tGATTCTCTTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGccc                    <  2:857160/140‑1 (MQ=255)

                                                                                                                ctctTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGccc                    <  1:170571/135‑1 (MQ=255)

                                                                                                                ctctTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGccc                    >  2:170571/1‑135 (MQ=255)

                                                                                                                ctctTCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTGTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGcccttccc               >  2:763745/1‑140 (MQ=255)

                                                                                                                   ttCGCATTTCGCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGcccttcccctt            >  1:329613/1‑140 (MQ=255)

                                                                                                                             gCCACCACCGCGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCCCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACaaa  >  2:855324/1‑140 (MQ=255)

ATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTACGGCGCTGAAACGCTCGCGCCCTTCCCCTTCAATCACAAA  >  NC_000913/1848271‑1848535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A