Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F28 I1 R1
|
126 |
72.5 |
2472046 |
99.4% |
2457213 |
138.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,997,140 |
(C)5→6 |
coding (675/753 nt) |
tcyN ← |
cystine ABC transporter ATP binding subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,997,135 | 1 | . | C | 100.0%
| 67.0
/ NA
| 20 | A227G (GCC→GGC) | tcyN | cystine ABC transporter ATP binding subunit |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (8/12); total (8/12) |
TTTATCAGGCTTGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGG‑CCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGAC > NC_000913/1996999‑1997268
|
tttATCAGGCTTGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGG‑ccc < 1:21333/140‑1 (MQ=255)
ttGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGa < 2:493404/140‑1 (MQ=255)
ttGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGa < 1:847292/140‑1 (MQ=255)
aTGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATc < 2:408977/140‑1 (MQ=255)
tCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGcccc < 1:673763/140‑1 (MQ=255)
ccAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCt < 1:137469/140‑1 (MQ=255)
aTCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGcc < 1:198595/140‑1 (MQ=255)
ggggCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGGCGGCAAAAAAAGCTTTTGCGGCCCCCCCCCTCGACTATCCGCCCCCGGGCCCTAAAAAACGCCCCGTcc > 2:922515/1‑140 (MQ=255)
ttCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCgg < 1:795257/140‑1 (MQ=255)
tattatTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCaa < 2:253884/140‑1 (MQ=255)
agcagAAACTTCTCGAGGAACTGGCGGGGGCGAGGCTGCTCGGGGGCGGCAAATAACGCTTTTGCGGCCCCCCCGCTCGACTATCCGCCCCTGGGCCATAAAAATCGCCCCGGCCGCCACATCCCCGGCAAAGCTCAttt > 2:225504/1‑140 (MQ=255)
cagAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAAGATCCCGGGCAAAGCTCATTTCg > 1:354529/1‑140 (MQ=255)
gAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGGCGGCAAATAAAGCTTTTTCGGCCCCCCCGCTCGACTATCCGCCCCCGGGCCATAAAAATCGCCCCGGCCGCAACATCCCCGGCAAAGCTCATTTCGTg > 2:673911/1‑140 (MQ=255)
aaCTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCg < 1:922515/140‑1 (MQ=255)
aaCTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCg < 1:1036630/140‑1 (MQ=255)
gAGGAACTGGCGGGTGCGAGGCTTCTCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGGCCATAAAGATCGCCCCGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATc > 1:1130045/1‑140 (MQ=255)
tgctCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTgcg < 1:819523/140‑1 (MQ=255)
ctCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGcc > 2:117780/1‑140 (MQ=255)
ctCGGGGTCGGCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGcc > 1:1048644/1‑140 (MQ=255)
gCAAATAACGCTTTTGCGGCCCCCCTGCTCGACTATCCCCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGa > 1:328878/1‑140 (MQ=255)
tttGCGGCCCCCCTGCTCGACTATCCGCCCCTGATCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGa < 2:1068677/140‑1 (MQ=255)
ttGCGGCCCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGAc > 2:1106578/1‑140 (MQ=255)
|
TTTATCAGGCTTGAAATGGCGTCCAGCCCCGACAGGTGAATCGTCGGGGCTGATTTTTTCTTATTATTGCAGCAGAAACTTCTCGAGGAACTGGCGGGTGCGAGGCTGCTCGGGGTCGGCAAATAACGCTTTTGCGG‑CCCCCTGCTCGACTATCCGCCCCTGGTCCATAAAGATCGCCCGGTCCGCAACATCCCGGGCAAAGCTCATTTCGTGCGTCACAATCACCATCGTGCGCTTTTCCTGCGCCAGCTGACGGATGGTGTTCAGGAC > NC_000913/1996999‑1997268
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
