Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,767	A→G	K250K (AAA→AAG)	arnD →	putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,767	0	A	G	100.0%	192.6 / NA	52	K250K (AAA→AAG)	arnD	putative 4‑deoxy‑4‑formamido‑L‑arabinose‑phosphoundecaprenol deformylase ArnD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/24); total (28/24)

TCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAGT  >  NC_000913/2370632‑2370902

tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:101993/140‑1 (MQ=255)

tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:902366/140‑1 (MQ=255)

tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:827668/140‑1 (MQ=255)

tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     >  2:827668/1‑140 (MQ=255)

tCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGcgcg                                                                                                                                     <  1:331407/140‑1 (MQ=255)

 cGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAgcgcgc                                                                                                                                    >  2:399338/1‑140 (MQ=255)

  gggATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCa                                                                                                                                   <  2:715291/140‑1 (MQ=255)

   ggATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAg                                                                                                                                  >  1:1191532/1‑140 (MQ=255)

         gAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAgg                                                                                                                            >  2:50237/1‑140 (MQ=255)

              cAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAgg                                                                                                                            >  2:883689/1‑135 (MQ=255)

              cAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAgg                                                                                                                            <  1:883689/135‑1 (MQ=255)

                         aaCGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTg                                                                                                            <  1:399338/140‑1 (MQ=255)

                          aCGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGc                                                                                                           <  2:304280/140‑1 (MQ=255)

                             gttggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCt                                                                                                        >  1:934396/1‑140 (MQ=255)

                              ttggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCtt                                                                                                       <  1:1146365/140‑1 (MQ=255)

                               tggttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCttt                                                                                                      >  2:122632/1‑140 (MQ=255)

                                 gttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTaa                                                                                                    >  2:968857/1‑140 (MQ=255)

                                 gttACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTaa                                                                                                    <  1:50237/140‑1 (MQ=255)

                                     cTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGa                                                                                                <  2:1149581/140‑1 (MQ=255)

                                      tCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGaa                                                                                               <  2:934396/140‑1 (MQ=255)

                                      tCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGaa                                                                                               <  1:968857/140‑1 (MQ=255)

                                          ccGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTg                                                                                                            <  1:822838/123‑1 (MQ=255)

                                          ccGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTg                                                                                                            >  2:822838/1‑123 (MQ=255)

                                             cATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGc                                                                                                  >  1:75138/1‑130 (MQ=255)

                                             cATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGc                                                                                                  <  2:75138/130‑1 (MQ=255)

                                               tCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAAc                                                                                              <  1:373573/132‑1 (MQ=255)

                                               tCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAAc                                                                                              >  2:373573/1‑132 (MQ=255)

                                                ccTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCg                                                                                                 >  2:286004/1‑128 (MQ=255)

                                                ccTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCg                                                                                                 <  1:286004/128‑1 (MQ=255)

                                                 cTGCGAGATAAAGGCACGCCGG‑TTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCagag                                                                                   <  1:100578/140‑1 (MQ=255)

                                                             ggCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCt                                                                        <  2:361964/140‑1 (MQ=255)

                                                                 cGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGa                                                                    >  1:440054/1‑140 (MQ=255)

                                                                           taCCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTc                                                          <  2:440054/140‑1 (MQ=255)

                                                                               aTTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCgg                                                      <  2:1191532/140‑1 (MQ=255)

                                                                                ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  1:72325/1‑140 (MQ=255)

                                                                                ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  2:185737/1‑140 (MQ=255)

                                                                                ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  2:197241/1‑140 (MQ=255)

                                                                                ttCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGa                                                     >  2:442882/1‑140 (MQ=255)

                                                                                        gAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGc                                             >  1:1046615/1‑140 (MQ=255)

                                                                                           gTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCgg                                          >  2:980498/1‑140 (MQ=255)

                                                                                              gAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACg                                       >  1:939451/1‑140 (MQ=255)

                                                                                               aaGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGt                                      >  2:554784/1‑140 (MQ=255)

                                                                                                   cTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAg                                  >  1:458931/1‑140 (MQ=255)

                                                                                                      cgcTTATCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAggct                               >  2:548121/1‑140 (MQ=255)

                                                                                                           aTCAGCATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGgctggctg                          >  2:586294/1‑140 (MQ=255)

                                                                                                                cATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTg                     >  2:762206/1‑140 (MQ=255)

                                                                                                                cATAATTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTg                     >  1:375815/1‑140 (MQ=255)

                                                                                                                    aTTTTGTGGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCcaa                 <  1:273344/140‑1 (MQ=255)

                                                                                                                         gtgGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAAt            >  1:835791/1‑140 (MQ=255)

                                                                                                                          tgGATCTCCTCAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAAtt           <  2:939451/140‑1 (MQ=255)

                                                                                                                                tcctcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGt     >  1:369071/1‑140 (MQ=255)

                                                                                                                                  ctcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAg   >  1:62943/1‑140 (MQ=255)

                                                                                                                                  ctcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAg   >  1:1198586/1‑140 (MQ=255)

                                                                                                                                   tcAAGCGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAGt  <  2:1046615/140‑1 (MQ=255)

TCGGGATGTGAAAGCAGAAGATTTTAACGGTTGGTTACTCAACCGCATCCTGCGAGATAAAGGCACGCCGGTTTATACCATTCATGCAGAAGTTGAAGGCTGCGCTTATCAGCATAATTTTGTGGATCTCCTCAAACGCGCAGCTCAGGAAGGCGTGACATTTTGCCCTTTAAGCGAACTGTTATCAGAGACGTTGCCGCTCGGACAAGTTGTTCGCGGAAATATTGCCGGACGTGAAGGCTGGCTGGGTTGCCAACAAATTGCGGGTAGT  >  NC_000913/2370632‑2370902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A