Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F28 I1 R1 126 72.5 2472046 99.4% 2457213 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,611,639 T→C D61D (GAT→GAC)  hyfJ → putative hydrogenase 4 assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,611,6390TC100.0% 192.9 / NA 52D61D (GAT→GAC) hyfJputative hydrogenase 4 assembly protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

GTCGCCAGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACG  >  NC_000913/2611508‑2611774
                                                                                                                                   |                                                                                                                                       
gTCGCCAGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGc                                                                                                                                 <  1:1076583/140‑1 (MQ=255)
 tCGCCAGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCa                                                                                                                                <  1:1006771/140‑1 (MQ=255)
 tCGCCAGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCa                                                                                                                                >  1:577750/1‑140 (MQ=255)
     cAGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTgg                                                                                                                            <  1:979029/140‑1 (MQ=255)
      aGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGt                                                                                                                           <  2:465383/140‑1 (MQ=255)
                              aGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTaa                                                                                                   <  2:577750/140‑1 (MQ=255)
                              aGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAACAAGCCCGACGTaa                                                                                                   <  2:145342/140‑1 (MQ=255)
                               gCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAg                                                                                                  <  1:286933/140‑1 (MQ=255)
                                ctctCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGa                                                                                                 >  1:277958/1‑140 (MQ=255)
                                ctctCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGa                                                                                                 >  1:308732/1‑140 (MQ=255)
                                    cAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCt                                                                                             <  2:308732/140‑1 (MQ=255)
                                     aGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTg                                                                                            >  1:1158930/1‑140 (MQ=255)
                                     aGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTg                                                                                            >  2:805791/1‑140 (MQ=255)
                                               ttACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGtgatga                                                                                  >  1:957856/1‑140 (MQ=255)
                                                 aCTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACt                                                                                <  1:215279/140‑1 (MQ=255)
                                                 aCTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACt                                                                                >  1:591818/1‑140 (MQ=255)
                                                 aCTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGGGATGACt                                                                                >  2:1047138/1‑140 (MQ=255)
                                                  cTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACtt                                                                               >  2:605736/1‑140 (MQ=255)
                                                  cTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACtt                                                                               >  2:746553/1‑140 (MQ=255)
                                                     gctagctaTCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTg                                                                            >  2:74320/1‑140 (MQ=255)
                                                      ctagctaTCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTgg                                                                           <  1:605736/140‑1 (MQ=255)
                                                       tagctaTCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGt                                                                          <  2:957856/140‑1 (MQ=255)
                                                            aTCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGAt                                                                     >  2:1153899/1‑140 (MQ=255)
                                                                 ccATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTa                                                                >  1:808281/1‑140 (MQ=255)
                                                                  cATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTAt                                                               <  2:808281/140‑1 (MQ=255)
                                                                   aTCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTAtt                                                              >  2:555524/1‑140 (MQ=255)
                                                                     cACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGa                                                            >  1:1189868/1‑140 (MQ=255)
                                                                      aCGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGAc                                                           <  1:74320/140‑1 (MQ=255)
                                                                           ggCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATACTGGGGGTGATGACTTTTGGTGAGATTGTTATTGGCgccag                                                      >  1:1086537/1‑140 (MQ=255)
                                                                               tgattgatTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCcac                                                  >  1:214166/1‑140 (MQ=255)
                                                                               tgattgatTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCcac                                                  >  2:989735/1‑140 (MQ=255)
                                                                               tgattgatTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCcac                                                  >  1:153033/1‑140 (MQ=255)
                                                                                  ttgattgTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACAcc                                               >  1:133936/1‑140 (MQ=255)
                                                                                   tgattgTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATACTGGGGGTGATGACTTTTGGTGAGATTGTTATTGGCGCCAGCCACACCg                                              <  2:1086537/140‑1 (MQ=255)
                                                                                             aaTGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGAc                                    <  1:1153899/140‑1 (MQ=255)
                                                                                                   gCCTTCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGc                              >  1:863152/1‑140 (MQ=255)
                                                                                                      ttCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCAtt                           <  2:1189868/140‑1 (MQ=255)
                                                                                                       tCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTc                          >  1:787803/1‑140 (MQ=255)
                                                                                                       tCCGCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTc                          >  2:991434/1‑140 (MQ=255)
                                                                                                          gCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTcgcg                       <  1:555524/140‑1 (MQ=255)
                                                                                                          gCTGCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTcgcg                       <  2:1158930/140‑1 (MQ=255)
                                                                                                             gCCCACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCa                    <  1:574046/140‑1 (MQ=255)
                                                                                                              cccACTGACGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCAc                   >  2:153963/1‑140 (MQ=255)
                                                                                                                     aCGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGa            <  1:879386/140‑1 (MQ=255)
                                                                                                                      cGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTcgcg                       >  2:737080/1‑128 (MQ=255)
                                                                                                                      cGGAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTcgcg                       <  1:737080/128‑1 (MQ=255)
                                                                                                                        gAATACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGa         <  1:805791/140‑1 (MQ=255)
                                                                                                                           tACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGAcgc      >  1:512188/1‑140 (MQ=255)
                                                                                                                           tACGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGAcgc      <  1:989735/140‑1 (MQ=255)
                                                                                                                             cGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGa    >  1:532362/1‑140 (MQ=255)
                                                                                                                             cGAAGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGa    >  1:1001189/1‑140 (MQ=255)
                                                                                                                               aaGACTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACg  >  1:364734/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GTCGCCAGTAGCGACGAGATGCCGGAACACAGCTCTCAGGTAATGTATTACTCGCTAGCTATCGGCCATCACGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACG  >  NC_000913/2611508‑2611774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A