Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F28 I1 R1
|
138 |
62.6 |
2119710 |
99.4% |
2106991 |
138.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,613,481 |
T→C |
L528L (TTA→CTA) |
hyfR → |
DNA‑binding transcriptional activator HyfR |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,613,481 | 0 | T | C | 97.1%
| 117.3
/ ‑4.3
| 34 | L528L (TTA→CTA) | hyfR | DNA‑binding transcriptional activator HyfR |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (20/13); total (20/14) |
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 |
CATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCG > NC_000913/2613348‑2613617
|
cATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCaa < 2:733051/140‑1 (MQ=255)
aTTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCaaa > 2:467332/1‑140 (MQ=255)
caccaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCa > 2:281620/1‑140 (MQ=255)
accaACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCAc < 2:131999/140‑1 (MQ=255)
tGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCaaaaaa > 1:90408/1‑140 (MQ=255)
gtgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgc < 1:850353/140‑1 (MQ=255)
tgGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgcg > 1:757952/1‑140 (MQ=255)
aaaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCat > 2:62246/1‑140 (MQ=255)
aaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgc < 1:109761/134‑1 (MQ=255)
aaTGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGgcgc > 2:109761/1‑134 (MQ=255)
gAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcg < 1:169814/140‑1 (MQ=255)
aaGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcg < 2:1026414/139‑1 (MQ=255)
aaGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcg > 1:1026414/1‑139 (MQ=255)
gTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcgcg < 2:937891/131‑1 (MQ=255)
gTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATcgcg > 1:937891/1‑131 (MQ=255)
ttATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCAc < 1:467332/140‑1 (MQ=255)
tCGCCTGAATGTCTTCCCACTGGGATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTAc > 2:625570/1‑140 (MQ=255)
tGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAg > 1:59308/1‑140 (MQ=255)
tGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAg > 1:543647/1‑140 (MQ=255)
tGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAg > 2:433545/1‑140 (MQ=255)
aTGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTg < 2:757952/140‑1 (MQ=255)
tCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCg < 2:131320/140‑1 (MQ=255)
tGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGAt > 1:389723/1‑140 (MQ=255)
tGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGAt > 1:363058/1‑140 (MQ=255)
gAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTg > 1:633269/1‑140 (MQ=255)
cgcgACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTgcg < 2:184772/140‑1 (MQ=255)
cgcgACCGTCCGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTgcg < 2:59308/140‑1 (MQ=255)
cGGAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGaa > 2:833521/1‑140 (MQ=255)
gAAGATATCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGaaaa > 2:994661/1‑140 (MQ=255)
atCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGAt > 1:464820/1‑140 (MQ=255)
atCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGAt > 2:905634/1‑140 (MQ=255)
atCCCTCTTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGAt > 1:928106/1‑140 (MQ=255)
ctTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGGCCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAgcg < 1:153758/140‑1 (MQ=255)
ctTCTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAgcg < 1:625570/140‑1 (MQ=255)
|
CATTGCCGCCACCAACCGTGATTTGTGGCAAATGGTTGAAGATCGCCAGTTTCGCAGCGATCTCTTTTATCGCCTGAATGTCTTCCCACTGGAATTGCCGCCGCTACGCGACCGTCCGGAAGATATCCCTCTTTTAGCAAAACATTTCACGCAAAAAATGGCGCGCCATATGAATCGCGCAATTGACGCCATCCCGACCGAGGCACTACGCCAGTTGATGTCGTGGGATTGGCCGGGCAACGTGCGCGAGCTGGAAAACGTGATTGAGCG > NC_000913/2613348‑2613617
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
