Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,703,236	G→A	H51Y (CAT→TAT)	era ←	30S ribosomal subunit maturation GTPase Era

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,703,236	0	G	A	100.0%	134.6 / NA	38	H51Y (CAT→TAT)	era	30S ribosomal subunit maturation GTPase Era
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/21); total (17/21)

AAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAG  >  NC_000913/2703107‑2703366

aaaTCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCcaca                                                                                                                          <  1:1174467/140‑1 (MQ=255)

             aaCATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             <  1:1184653/140‑1 (MQ=255)

             aaCATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             <  1:64433/140‑1 (MQ=255)

                    ccAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTg                                                                                                      <  2:641008/140‑1 (MQ=255)

                    ccAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTg                                                                                                      >  1:156968/1‑140 (MQ=255)

                        tAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGcc                                                                                                  >  1:592379/1‑140 (MQ=255)

                           agCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             >  2:1007893/1‑126 (MQ=255)

                           agCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAg                                                                                                             <  1:1007893/126‑1 (MQ=255)

                             ctgctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCg                                                                                             >  2:684434/1‑140 (MQ=255)

                             ctgctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCg                                                                                             <  1:167453/140‑1 (MQ=255)

                                ctCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGa                                                                                          >  2:329623/1‑140 (MQ=255)

                                 tCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGaa                                                                                         <  2:156968/140‑1 (MQ=255)

                                  cgccgcTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAg                                                                                        >  2:1074613/1‑140 (MQ=255)

                                               atcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGAttt                                                                            >  1:968449/1‑139 (MQ=255)

                                               atcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGAttt                                                                            <  2:968449/139‑1 (MQ=255)

                                                tcaGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTc                                                                          <  1:588111/140‑1 (MQ=255)

                                                          aaTGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                                                <  2:592379/140‑1 (MQ=255)

                                                            tGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGt                                                              <  1:684434/140‑1 (MQ=255)

                                                                  gTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTc                                                        >  1:310705/1‑140 (MQ=255)

                                                                   tttttCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                                                >  1:997368/1‑131 (MQ=255)

                                                                   tttttCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCcagca                                                                <  2:997368/131‑1 (MQ=255)

                                                                         ttcCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAAtg                                                 <  1:963765/140‑1 (MQ=255)

                                                                             atatGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGa                                             <  2:994295/140‑1 (MQ=255)

                                                                                     gCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCaa                                     >  1:17161/1‑140 (MQ=255)

                                                                                          gtgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGtt                                 >  1:963616/1‑139 (MQ=255)

                                                                                          gtgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGtt                                 <  2:963616/139‑1 (MQ=255)

                                                                                          gtgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTc                                >  1:1075708/1‑140 (MQ=255)

                                                                                           tgtATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCg                               <  2:17161/140‑1 (MQ=255)

                                                                                             tATCGACGTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGCGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGa                             <  2:1075708/140‑1 (MQ=255)

                                                                                                    gTAGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcga                      >  2:125919/1‑140 (MQ=255)

                                                                                                      aGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcgacg                    <  2:371594/140‑1 (MQ=255)

                                                                                                      aGATCGCCTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCcgacg                    <  1:125919/140‑1 (MQ=255)

                                                                                                            ccTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCa              >  2:140039/1‑140 (MQ=255)

                                                                                                             cTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGa                             <  2:1032442/124‑1 (MQ=255)

                                                                                                             cTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGa                             >  1:1032442/1‑124 (MQ=255)

                                                                                                             cTGATACGCGCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCaa             >  2:954142/1‑140 (MQ=255)

                                                                                                                   cgcgCCTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATc       <  1:433851/140‑1 (MQ=255)

                                                                                                                        cTTCAGTATAGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAg  >  1:1041040/1‑140 (MQ=255)

AAATCACCAGCTCAACATCGCCAATAGAGCTGCTCGCCGCTTTGTTCATCAGGCGGTTAATGGCGCGTTTTTCTTCCATATGCAGGCCCGGTGTATCGACGTAGATCGCCTGATACGCGCCTTCAGTATGGATCCCCACAATGCGGTGACGAGTTGTCTGCGCCTTGCGGGAAGTGATGGAGATTTTCTGCCCCAGCAGTTTGTTCAACAATGTGGATTTGCCAACGTTCGGACGTCCGACGATGGCAATAAATCCGCAG  >  NC_000913/2703107‑2703366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A