Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	72.5	2472046	99.4%	2457213	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,639,576	(A)_6→5	coding (145/336 nt)	uspB ←	putative universal stress (ethanol tolerance) protein B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,639,571	0	A	.	100.0%	340.8 / NA	78	coding (150/336 nt)	uspB	putative universal stress (ethanol tolerance) protein B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (38/40); total (38/40)

CACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATG  >  NC_000913/3639431‑3639704

cacaATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGAT‑ACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTa                                                                                                                                       >  2:550815/1‑140 (MQ=255)

   aaTGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaa                                                                                                                                     >  2:388211/1‑140 (MQ=255)

         cTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTaaaaagccc                                                                                                                               <  1:264725/140‑5 (MQ=255)

          tGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGcccc                                                                                                                             <  1:882217/140‑1 (MQ=255)

          tGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGcccc                                                                                                                             >  2:400561/1‑140 (MQ=255)

          tGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGcccc                                                                                                                             <  2:376819/140‑1 (MQ=255)

           ggTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            <  1:514664/140‑1 (MQ=255)

           ggTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            >  2:83759/1‑140 (MQ=255)

            gTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            >  1:1189601/1‑139 (MQ=255)

            gTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGccccc                                                                                                                            <  2:1189601/139‑1 (MQ=255)

               aGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCa                                                                                                                        >  1:200648/1‑140 (MQ=255)

                 aaTAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATc                                                                                                                      <  1:245132/140‑1 (MQ=255)

                 aaTAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATc                                                                                                                      <  1:1110343/140‑1 (MQ=255)

                   tAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCaa                                                                                                                    <  2:200648/140‑1 (MQ=255)

                     aaCTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACa                                                                                                                  <  2:619185/140‑1 (MQ=255)

                      aCTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACat                                                                                                                 <  1:665646/140‑1 (MQ=255)

                           cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  2:740216/1‑140 (MQ=255)

                           cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            <  1:144864/140‑1 (MQ=255)

                           cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  1:1168560/1‑140 (MQ=255)

                           cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  2:502726/1‑140 (MQ=255)

                           cGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGa                                                                                                            >  2:1095947/1‑140 (MQ=255)

                             aCGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATa                                                                                                          >  2:168756/1‑140 (MQ=255)

                                  cccGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCa                                                                                                     <  2:609672/140‑1 (MQ=255)

                                    cGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAAt                                                                                                   >  1:218378/1‑140 (MQ=255)

                                              ggcgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGt                                                                                         <  1:740216/140‑1 (MQ=255)

                                                cgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAg                                                                                          <  1:804330/137‑1 (MQ=255)

                                                cgAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAg                                                                                          >  2:804330/1‑137 (MQ=255)

                                                 gAATAAATTCATCATCGTGATGATCGCGAT‑ACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     <  1:550815/140‑1 (MQ=255)

                                                  aaTAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     <  1:866890/140‑1 (MQ=255)

                                                    tAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa                                                                                   <  1:388211/140‑1 (MQ=255)

                                                    tAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCa                                                                                   <  1:672092/140‑1 (MQ=255)

                                                      aaTTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATGGAGCAATGGATCGCAGTTACGCAGt                                                                                 <  1:1054786/140‑1 (MQ=255)

                                                      aaTTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGt                                                                                 <  1:90333/140‑1 (MQ=255)

                                                               tcGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaac                                                                        <  1:445580/140‑1 (MQ=255)

                                                                cGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaaca                                                                       <  1:420834/140‑1 (MQ=255)

                                                                cGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaaca                                                                       >  2:856680/1‑140 (MQ=255)

                                                                  tgatgaTCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTaacaac                                                                     >  2:910618/1‑140 (MQ=255)

                                                                    atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     >  2:865419/1‑122 (MQ=255)

                                                                    atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACg                                                                                     <  1:865419/122‑1 (MQ=255)

                                                                    atgatCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgc                                                                   >  2:317670/1‑140 (MQ=255)

                                                                       atCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcgcg                                                                <  2:1168560/140‑1 (MQ=255)

                                                                         cgcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTa                                                              >  2:702145/1‑140 (MQ=255)

                                                                          gcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAg                                                             <  1:168756/140‑1 (MQ=255)

                                                                          gcgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAg                                                             <  2:218378/140‑1 (MQ=255)

                                                                           cgATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGt                                                            >  2:1012942/1‑140 (MQ=255)

                                                                              tAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGtgat                                                         <  1:709801/140‑1 (MQ=255)

                                                                                      ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg                                                                  >  1:309413/1‑123 (MQ=255)

                                                                                      ggCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAAcgcg                                                                  <  2:309413/123‑1 (MQ=255)

                                                                                          tAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             <  1:1012942/140‑1 (MQ=255)

                                                                                          tAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             <  1:718820/140‑1 (MQ=255)

                                                                                           aGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcg                                            >  2:959673/1‑140 (MQ=255)

                                                                                            gATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           <  1:1072521/140‑1 (MQ=255)

                                                                                            gATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           <  1:910618/140‑1 (MQ=255)

                                                                                             atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             >  2:1071068/1‑137 (MQ=255)

                                                                                             atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgc                                             <  1:1071068/137‑1 (MQ=255)

                                                                                             atatACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGcc                                          <  2:1041868/140‑1 (MQ=255)

                                                                                               ataCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCAt                                        <  1:375857/140‑1 (MQ=255)

                                                                                                taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa                                         <  2:759887/138‑1 (MQ=255)

                                                                                                taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCa                                         >  1:759887/1‑138 (MQ=255)

                                                                                                taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATg                                       >  1:530124/1‑140 (MQ=255)

                                                                                                taCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATg                                       >  1:332319/1‑140 (MQ=255)

                                                                                                 aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGt                                      >  2:1105919/1‑140 (MQ=255)

                                                                                                 aCCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGt                                      >  2:197774/1‑140 (MQ=255)

                                                                                                  ccAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGtt                                     >  2:1189950/1‑140 (MQ=255)

                                                                                                     aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  >  1:904458/1‑140 (MQ=255)

                                                                                                     aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  >  1:324843/1‑140 (MQ=255)

                                                                                                     aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  <  1:702145/140‑1 (MQ=255)

                                                                                                     aaCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAAc                                  >  2:752252/1‑140 (MQ=255)

                                                                                                      aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:36444/1‑140 (MQ=255)

                                                                                                      aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:601365/1‑140 (MQ=255)

                                                                                                      aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:676156/1‑140 (MQ=255)

                                                                                                      aCGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACa                                 >  1:140766/1‑140 (MQ=255)

                                                                                                       cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           <  1:444780/129‑1 (MQ=255)

                                                                                                       cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAgcgcgc                                           >  2:444780/1‑129 (MQ=255)

                                                                                                       cGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACaa                                >  1:1215472/1‑140 (MQ=255)

                                                                                                          gaCGCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGc                             >  2:999674/1‑140 (MQ=255)

                                                                                                             gCACCTGTTTGTTGGGTTGGCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCaaa                          <  1:1095947/140‑1 (MQ=255)

                                                                                                                         tgggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAg              >  2:369150/1‑140 (MQ=255)

                                                                                                                           ggttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGcc            <  1:502726/140‑1 (MQ=255)

                                                                                                                             ttggCCATGTGAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCa          <  1:317670/140‑1 (MQ=255)

                                                                                                                                     gtgAGGT‑AAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATg  >  2:671138/1‑140 (MQ=255)

CACAATGCGCTGGTCAGAATAAACTGCCGACGCACCCGCTCACAGCGGCGAATAAATTCATCATCGTGATGATCGCGATAACGTTGGGCATAGATATACCAAACGAGACGCACCTGTTTGTTGGGTTGGCCATGTGAGGTAAAAAAGCCCCCTCCATCAACATATTGATAGAGCAATGGATCGCAGTTACGCAGTACCACTAACAACGCGCGTAGTGATGAGAAATAGCGCGCCATGTTAACAATGCAAACGACACATAAAGCCCAAAATAATG  >  NC_000913/3639431‑3639704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A