Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	545,941	A→G	D141D (GAT→GAC)	allD ←	ureidoglycolate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	545,941	0	A	G	100.0%	168.9 / NA	47	D141D (GAT→GAC)	allD	ureidoglycolate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/26); total (21/26)

TTCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCA  >  NC_000913/545814‑546076

ttCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGcac                                                                                                                             >  2:656485/1‑140 (MQ=255)

                 gTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGaa                                                                                                            >  2:133987/1‑140 (MQ=255)

                  tAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTTGGACTGGCACATCGAAATGCCAATGAAt                                                                                                           >  2:746908/1‑140 (MQ=255)

                  tAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAAt                                                                                                           >  2:282063/1‑140 (MQ=255)

                    gTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATcc                                                                                                         >  1:838185/1‑140 (MQ=255)

                       gCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCTAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgc                                                                                                      >  2:182016/1‑140 (MQ=255)

                            atCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 >  1:802601/1‑140 (MQ=255)

                            atCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 >  1:473860/1‑140 (MQ=255)

                                   gTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctg                                                                                          <  1:26698/140‑1 (MQ=255)

                                    tAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctgc                                                                                         <  1:133987/140‑1 (MQ=255)

                                      aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    >  1:467126/1‑127 (MQ=255)

                                      aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    <  2:467126/127‑1 (MQ=255)

                                      aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    <  2:741485/127‑1 (MQ=255)

                                      aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGgcgc                                                                                                    >  1:741485/1‑127 (MQ=255)

                                      aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCctgctg                                                                                       <  2:326453/140‑1 (MQ=255)

                                      aGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCctgctg                                                                                       <  1:746908/140‑1 (MQ=255)

                                       ggATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCtgctgc                                                                                      >  1:850686/1‑140 (MQ=255)

                                        gATCTCGTCGCCTTCTCCCGGCGCGGAAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGca                                                                                     <  2:603450/140‑1 (MQ=255)

                                          tctcGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGcaca                                                                                   <  1:234090/140‑1 (MQ=255)

                                           ctcGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACaa                                                                                  >  2:405921/1‑140 (MQ=255)

                                             cgtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctgc                                                                                         >  1:690944/1‑131 (MQ=255)

                                             cgtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGctgcctgc                                                                                         <  2:690944/131‑1 (MQ=255)

                                              gtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 <  1:458901/122‑1 (MQ=255)

                                              gtcgCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCggg                                                                                                 >  2:458901/1‑122 (MQ=255)

                                                    ttCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAgaga                                                                         <  1:86926/140‑1 (MQ=255)

                                                         ccGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTgcg                                                                    <  2:347886/140‑1 (MQ=255)

                                                         ccGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTgcg                                                                    <  2:850686/140‑1 (MQ=255)

                                                            gcgcGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGcgccg                                                                 >  1:198326/1‑140 (MQ=255)

                                                                   aaaGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGAc                                                          <  2:802601/140‑1 (MQ=255)

                                                                   aaaGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGAc                                                          <  2:473860/140‑1 (MQ=255)

                                                                       gCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCTAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCAt                                                      <  1:182016/140‑1 (MQ=255)

                                                                       gCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCAt                                                      <  1:532609/140‑1 (MQ=255)

                                                                            ggggTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGc                                                 <  2:198326/140‑1 (MQ=255)

                                                                                 tAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATa                                            >  2:758741/1‑140 (MQ=255)

                                                                                 tAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATa                                            <  1:282063/140‑1 (MQ=255)

                                                                                      ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGTACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGac                                       >  2:347160/1‑140 (MQ=255)

                                                                                      ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATAcc                                          >  2:174646/1‑137 (MQ=255)

                                                                                      ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATAcc                                          <  1:174646/137‑1 (MQ=255)

                                                                                      ccGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGac                                       <  1:656485/140‑1 (MQ=255)

                                                                                        gtagtaAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGacca                                     >  2:821657/1‑140 (MQ=255)

                                                                                        gtagtaAATTTCCGCGCCGCCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAACGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGacca                                     >  1:137028/1‑140 (MQ=255)

                                                                                                      cgccgcCAAACGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCAttt                       >  1:319912/1‑140 (MQ=255)

                                                                                                              aaCGGCACCACCATTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGc               <  2:790894/140‑1 (MQ=255)

                                                                                                                     ccaccaTTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTg        <  1:244488/140‑1 (MQ=255)

                                                                                                                     ccaccaTTGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTg        <  1:821657/140‑1 (MQ=255)

                                                                                                                           ttGGGTCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCa  <  2:319912/140‑1 (MQ=255)

                                                                                                                           ttGGGTCGGACTGGCACATCGAAATGCCAACGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCa  <  2:137028/140‑1 (MQ=255)

TTCCCCATGCCTGTACGGTAGTCGCCATATCAAAGGTAAGGATCTCGTCGCCTTCTCCCGGCGCGGCAAAGGCCAGGGGGTTAGTACCGTAGTAAATTTCCGCGCCGCCAAACGGCACCACCATTGGATCGGACTGGCACATCGAAATGCCAATGAATCCGGCGCGGGCTGCCTGCTGCACAAAATAAGAGATTGCGCCGCTGTGACCCATCCGGCTGATACCGACCACCGCAACGCCATTTTGCTGGGCGGTTTTGATGGCA  >  NC_000913/545814‑546076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A