Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	932,991	(G)_5→6	coding (397/495 nt)	lrp →	DNA‑binding transcriptional dual regulator Lrp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	932,986	1	.	G	100.0%	160.5 / NA	44	L131R (CTG→CGG)	lrp	DNA‑binding transcriptional dual regulator Lrp
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (21/23); total (21/23)

GGCGCACCGGATGTGTTTGAACAATTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCT‑GGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTA  >  NC_000913/932847‑933115

ggCGCACCGGATGTGTTTGAACAATTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCt                                                                                                                                    <  2:211951/140‑1 (MQ=255)

 gcgcACCGGATGTGTTTGAACAATTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCT‑g                                                                                                                                  <  2:668627/140‑1 (MQ=255)

      ccGGATGTGTTTGAACAATTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCT‑gggggg                                                                                                                             <  1:798148/140‑2 (MQ=255)

                   aaCAATTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCg                                                                                                                 >  1:511758/1‑140 (MQ=255)

                       aTTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTg                                                                                                             >  2:112153/1‑140 (MQ=255)

                       aTTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGACTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTg                                                                                                             >  2:724497/1‑140 (MQ=255)

                        ttCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGc                                                                                                            <  1:290197/140‑1 (MQ=255)

                        ttCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGc                                                                                                            <  2:508771/140‑1 (MQ=255)

                         tCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGcc                                                                                                           >  1:671589/1‑140 (MQ=255)

                           aaTACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCatg                                                                                                                      >  2:598253/1‑127 (MQ=255)

                           aaTACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCatg                                                                                                                      <  1:598253/127‑1 (MQ=255)

                           aaTACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTg                                                                                                         <  2:53356/140‑1 (MQ=255)

                           aaTACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTg                                                                                                         <  1:606770/140‑1 (MQ=255)

                                cGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTc                                                                                                    >  2:416418/1‑140 (MQ=255)

                                  cTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCaa                                                                                                  >  2:498670/1‑140 (MQ=255)

                                   tGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAAt                                                                                                 <  1:127585/140‑1 (MQ=255)

                                       cAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGaca                                                                                             <  1:112153/140‑1 (MQ=255)

                                         aaaaCTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGacaca                                                                                           <  1:544889/140‑1 (MQ=255)

                                           aaCTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACg                                                                                         <  1:479847/140‑1 (MQ=255)

                                             cTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGa                                                                                       <  1:679792/140‑1 (MQ=255)

                                               tGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACa                                                                                     >  2:583565/1‑140 (MQ=255)

                                                   gaaATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACg                                                                                 <  1:583565/140‑1 (MQ=255)

                                                   gaaATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACg                                                                                 <  1:498670/140‑1 (MQ=255)

                                                    aaaTTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACgt                                                                                <  2:811508/140‑1 (MQ=255)

                                                                 tCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGgaagaa                                                                   >  1:745350/1‑140 (MQ=255)

                                                                       aGTGTCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAg                                                             <  2:397979/140‑1 (MQ=255)

                                                                         tATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGga                                                                       >  1:717909/1‑128 (MQ=255)

                                                                         tATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGga                                                                       <  2:717909/128‑1 (MQ=255)

                                                                         tATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAg                                                             >  1:346954/1‑138 (MQ=255)

                                                                         tATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAg                                                             <  2:346954/138‑1 (MQ=255)

                                                                         tATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCa                                                           >  1:739846/1‑140 (MQ=255)

                                                                         tATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCa                                                           >  1:602723/1‑140 (MQ=255)

                                                                           tCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGTCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCaga                                                         >  1:195825/1‑140 (MQ=255)

                                                                           tCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCaga                                                         >  1:590014/1‑140 (MQ=255)

                                                                            ccGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCagag                                                        >  1:43208/1‑140 (MQ=255)

                                                                               gTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTaa                                                     <  2:739846/140‑1 (MQ=255)

                                                                                   tttCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGt                                                 <  2:261961/140‑1 (MQ=255)

                                                                                     tCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCt                                               >  2:595259/1‑140 (MQ=255)

                                                                                               gTTGAAAACAAGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACCCGGACATACGTTGTTATGGAAGAAGTCAAACAGAGTAATCGTCTGGTTATTAAg                                     <  2:533390/140‑1 (MQ=255)

                                                                                                    aaaCACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGAcgcg                                <  2:671589/140‑1 (MQ=255)

                                                                                                     aaCACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACgcgc                               <  2:511758/140‑1 (MQ=255)

                                                                                                     aaCACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACgcgc                               <  1:467267/140‑1 (MQ=255)

                                                                                                             tGCCGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTGTTAAGACGCGCTAACACgg                       >  2:431344/1‑140 (MQ=255)

                                                                                                               ccGGATATGTCAGTCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGaa                     <  2:195825/140‑1 (MQ=255)

                                                                                                               ccGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGaa                     >  1:328385/1‑140 (MQ=255)

                                                                                                                cGGATATGTCAGCCTACCGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAAc                    <  1:416418/140‑1 (MQ=255)

                                                                                                                                 cGTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGt   >  1:665060/1‑140 (MQ=255)

                                                                                                                                  gTAAGTTGCTGGGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTa  >  1:617799/1‑140 (MQ=255)

GGCGCACCGGATGTGTTTGAACAATTCAATACCGCTGTACAAAAACTTGAAGAAATTCAGGAGTGTCATTTAGTATCCGGTGATTTCGACTACCTGTTGAAAACACGCGTGCCGGATATGTCAGCCTACCGTAAGTTGCT‑GGGGGAAACCCTGCTGCGTCTGCCTGGCGTCAATGACACACGGACATACGTTGTTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAACACGGAACAGGTGCAAAATCGGCGTA  >  NC_000913/932847‑933115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A