Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	950,582	G→A	R167C (CGC→TGC)	pflA ←	pyruvate formate‑lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	950,582	0	G	A	100.0%	134.0 / NA	39	R167C (CGC→TGC)	pflA	pyruvate formate‑lyase activating enzyme
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (18/21); total (18/21)

GCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACT  >  NC_000913/950446‑950715

gCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGAt                                                                                                                                    >  2:544779/1‑140 (MQ=255)

     ccATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCcaca                                                                                                                               >  1:355826/1‑140 (MQ=255)

               ttGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACAtt                                                                                                                     >  2:28855/1‑140 (MQ=255)

               ttGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACAtt                                                                                                                     >  2:789627/1‑140 (MQ=255)

                    cAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGt                                                                                                                <  1:612783/140‑1 (MQ=255)

                           tGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAg                                                                                                         <  2:76848/140‑1 (MQ=255)

                             gTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGat                                                                                                       <  1:704872/140‑1 (MQ=255)

                                 gggAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATAtt                                                                                                    <  1:779144/139‑1 (MQ=255)

                                 gggAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATAtt                                                                                                    >  2:779144/1‑139 (MQ=255)

                                  ggAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTa                                                                                                  >  2:39666/1‑140 (MQ=255)

                                      aaGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGACGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGa                                                                                              >  2:762188/1‑140 (MQ=255)

                                       aGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGaa                                                                                             >  2:217849/1‑140 (MQ=255)

                                         cTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACt                                                                                           <  1:306405/140‑1 (MQ=255)

                                               tttCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCg                                                                                     <  1:428971/140‑1 (MQ=255)

                                                 tctcAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTg                                                                                   <  1:28855/140‑1 (MQ=255)

                                                 tctcAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTg                                                                                   <  1:217849/140‑1 (MQ=255)

                                                  ctcAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGc                                                                                  <  2:4844/140‑1 (MQ=255)

                                                   tcAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCg                                                                                 <  2:95244/140‑1 (MQ=255)

                                                           gCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTgg                                                                         <  1:789627/140‑1 (MQ=255)

                                                            cccATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGa                                                                        >  1:519234/1‑140 (MQ=255)

                                                                    aCGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCaa                                                                <  1:39666/140‑1 (MQ=255)

                                                                      gggTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAAcc                                                              >  1:546268/1‑140 (MQ=255)

                                                                           aaTTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGAtt                                                         <  2:519234/140‑1 (MQ=255)

                                                                                  cGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTtggtgg                                                  >  1:202909/1‑140 (MQ=255)

                                                                                       cGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTtggt                                                    >  1:74755/1‑133 (MQ=255)

                                                                                       cGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTtggt                                                    <  2:74755/133‑1 (MQ=255)

                                                                                        gATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCtcg                                            <  2:546268/140‑1 (MQ=255)

                                                                                              cTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTc                                      <  1:544779/140‑1 (MQ=255)

                                                                                                gAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCAt                                    >  2:826702/1‑140 (MQ=255)

                                                                                                      tcatcgtcaGACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGttt                              >  2:157818/1‑140 (MQ=255)

                                                                                                           gtcaGTCCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGAt                         <  2:5474/140‑1 (MQ=255)

                                                                                                               gACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAg                     <  2:103679/140‑1 (MQ=255)

                                                                                                               gACCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAg                     <  1:324076/140‑1 (MQ=255)

                                                                                                                aCCAGCCTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGc                    >  1:518222/1‑140 (MQ=255)

                                                                                                                     ccTGGGACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTAc               >  1:332187/1‑140 (MQ=255)

                                                                                                                        gggACAACAACGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAg            <  2:518222/140‑1 (MQ=255)

                                                                                                                               caacGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGtt     >  2:405514/1‑140 (MQ=255)

                                                                                                                               caacGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGA‑TTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTa    >  2:70470/1‑140 (MQ=255)

                                                                                                                                  cGTAGCAGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACt  <  1:157818/140‑1 (MQ=255)

GCCACCCATTTGTGTTTGCCCAGCTCGTGGTAGGGGAGAAGCTCGATTTTCTCAACGTTGCCCATATCACGGGTAAATTCACCGAGGCGATGCGCTGAATCGTCATCGTCAGACCAGCCTGGGACAACAACGTAGCGGATCCACACCTTCACATTTTTGTTCGCCAGATATTTAGCGAACTCCAGCGTGCGGTGGTTGGAAACTCCAACCAGATTTTGGTGGATCTCGTCGTTCATCTGTTTGAGATCGAGCATTACCAGGTCGGTTACT  >  NC_000913/950446‑950715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A