Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F28 I1 R1	138	62.6	2119710	99.4%	2106991	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	951,980	A→G	C525C (TGT→TGC)	pflB ←	pyruvate formate‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	951,980	0	A	G	100.0%	293.5 / NA	79	C525C (TGT→TGC)	pflB	pyruvate formate‑lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (33/46); total (33/46)

ATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGA  >  NC_000913/951845‑952112

aTTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACg                                                                                                                                  >  2:234131/1‑140 (MQ=255)

 ttGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGc                                                                                                                                 <  2:1044005/140‑1 (MQ=255)

  tGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGcc                                                                                                                                <  1:234131/140‑1 (MQ=255)

     tACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATg                                                                                                                             >  1:645100/1‑140 (MQ=255)

     tACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATg                                                                                                                             >  1:872568/1‑140 (MQ=255)

      aCCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCa                                                                                                                               >  1:321589/1‑137 (MQ=255)

      aCCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCa                                                                                                                               <  2:321589/137‑1 (MQ=255)

       ccAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGt                                                                                                                           >  2:48033/1‑140 (MQ=255)

       ccAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGt                                                                                                                           >  2:13341/1‑140 (MQ=255)

       ccAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGt                                                                                                                           >  1:506107/1‑140 (MQ=255)

        cAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTg                                                                                                                          <  1:983534/140‑1 (MQ=255)

         aaaCTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACg                                                                                                                                  >  1:552006/1‑131 (MQ=255)

         aaaCTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACg                                                                                                                                  <  2:552006/131‑1 (MQ=255)

            cTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGa                                                                                                                      >  1:516538/1‑140 (MQ=255)

             tGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGAt                                                                                                                     <  1:52428/140‑1 (MQ=255)

             tGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGAt                                                                                                                     <  2:506107/140‑1 (MQ=255)

             tGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGAt                                                                                                                     <  2:615563/140‑1 (MQ=255)

                gggTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATTTTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAAc                                                                                                                  >  1:428620/1‑140 (MQ=255)

                    aTTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCa                                                                                                              <  2:233445/140‑1 (MQ=255)

                       cGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACgg                                                                                                           >  1:978181/1‑140 (MQ=255)

                           ttCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGt                                                                                                       <  2:789703/140‑1 (MQ=255)

                             cGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGc                                                                                                     <  2:327311/140‑1 (MQ=255)

                             cGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGc                                                                                                     >  1:772382/1‑140 (MQ=255)

                               aTTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAg                                                                                                   >  1:266116/1‑140 (MQ=255)

                               aTTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAg                                                                                                   <  2:403673/140‑1 (MQ=255)

                                tttCGAAGTCGATAGCCAGACCGTCCTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAgc                                                                                                  <  2:978181/140‑1 (MQ=255)

                                        tCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCag                                                                                          >  2:52738/1‑140 (MQ=255)

                                                cAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                                                                  <  1:427033/140‑1 (MQ=255)

                                                  gACCGTCTTCGTCACGAATCGGTTTAACTTGCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGGCAGACCAGCGATGCCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                                                                <  2:973799/140‑1 (MQ=255)

                                                    ccGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTa                                                                              >  2:802802/1‑140 (MQ=255)

                                                      gTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGc                                                                            <  2:655531/140‑1 (MQ=255)

                                                       tCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCt                                                                           >  1:518399/1‑140 (MQ=255)

                                                        cTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                                                                          >  1:749891/1‑140 (MQ=255)

                                                         ttCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGt                                                                         >  2:357191/1‑140 (MQ=255)

                                                          tCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                                                                        <  1:616412/140‑1 (MQ=255)

                                                           cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTAc                                                                       <  2:266116/140‑1 (MQ=255)

                                                                  aaTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATGCCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                                                                <  2:275472/140‑1 (MQ=255)

                                                                  aaTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                                                                <  1:357191/140‑1 (MQ=255)

                                                                  aaTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                                                                <  2:22414/140‑1 (MQ=255)

                                                                     cGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCa                                                             <  1:1045917/140‑1 (MQ=255)

                                                                       gTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg                                                           >  2:408933/1‑140 (MQ=255)

                                                                           aaCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGt                                                       <  1:13341/140‑1 (MQ=255)

                                                                             cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTgg                                                     <  2:172259/140‑1 (MQ=255)

                                                                              tttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga                                                    <  1:923212/140‑1 (MQ=255)

                                                                              tttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGga                                                    <  1:264393/140‑1 (MQ=255)

                                                                                tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg                                                  <  1:537983/140‑1 (MQ=255)

                                                                                 cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatga                                                 >  1:608570/1‑140 (MQ=255)

                                                                                 cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg                                                  >  1:867267/1‑139 (MQ=255)

                                                                                 cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatg                                                  <  2:867267/139‑1 (MQ=255)

                                                                                  gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatgat                                                >  1:436946/1‑140 (MQ=255)

                                                                                  gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatgat                                                >  2:416588/1‑140 (MQ=255)

                                                                                     tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt                                             <  2:772382/140‑1 (MQ=255)

                                                                                     tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGtt                                             <  2:518399/140‑1 (MQ=255)

                                                                                      atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTc                                            >  2:478619/1‑140 (MQ=255)

                                                                                       tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTca                                           <  2:608570/140‑1 (MQ=255)

                                                                                       tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTca                                           <  1:48033/140‑1 (MQ=255)

                                                                                          gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                        <  1:660430/140‑1 (MQ=255)

                                                                                          gATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtg                                        <  1:403189/140‑1 (MQ=255)

                                                                                             tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                     >  2:1059826/1‑140 (MQ=255)

                                                                                                  aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATg                                <  2:872568/140‑1 (MQ=255)

                                                                                                   cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGt                               <  1:802802/140‑1 (MQ=255)

                                                                                                      ggAGTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACt                            >  1:200187/1‑140 (MQ=255)

                                                                                                         gTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                         <  1:52738/140‑1 (MQ=255)

                                                                                                         gTCAGCAGCAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGtt                         >  2:275979/1‑140 (MQ=255)

                                                                                                            agcagcAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                     <  2:786614/125‑1 (MQ=255)

                                                                                                            agcagcAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTcagtgcag                                     >  1:786614/1‑125 (MQ=255)

                                                                                                            agcagcAACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag                      >  1:100050/1‑140 (MQ=255)

                                                                                                                gcaACGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccag                  <  2:200187/140‑1 (MQ=255)

                                                                                                                  aaCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc                <  1:1059826/140‑1 (MQ=255)

                                                                                                                  aaCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc                <  1:686528/140‑1 (MQ=255)

                                                                                                                  aaCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc                <  1:408933/140‑1 (MQ=255)

                                                                                                                   aCGGACAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAgccagcca               <  2:516538/140‑1 (MQ=255)

                                                                                                                       aCAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc           <  2:749891/140‑1 (MQ=255)

                                                                                                                       aCAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTcc           <  1:572763/140‑1 (MQ=255)

                                                                                                                        cAGACCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCa          <  2:804324/140‑1 (MQ=255)

                                                                                                                           aCCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa       >  2:871533/1‑140 (MQ=255)

                                                                                                                           aCCAGCGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa       >  1:225342/1‑140 (MQ=255)

                                                                                                                                cGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa  >  2:783337/1‑140 (MQ=255)

                                                                                                                                cGATACCGCACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa  >  2:969599/1‑140 (MQ=255)

ATTGTTACCAAACTGCGGGTATTCGCCTTCGATTTCGAAGTCGATAGCCAGACCGTCTTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATAACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGA  >  NC_000913/951845‑952112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A