Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,066,280	A→G	V232V (GTA→GTG)	agp →	glucose‑1‑phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,066,280	0	A	G	100.0%	120.9 / NA	35	V232V (GTA→GTG)	agp	glucose‑1‑phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/14); total (21/14)

CTATAAAGATTCCCCTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGC  >  NC_000913/1066151‑1066400

cTATAAAGATTCCCCTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTa                                                                                                                <  1:108725/140‑1 (MQ=255)

           ccccTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAatat                                                                                                     >  2:370839/1‑140 (MQ=255)

               tgcctgTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACg                                                                                                 >  1:129963/1‑140 (MQ=255)

                 cctgTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGaa                                                                                               >  1:241326/1‑140 (MQ=255)

                      aaaGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGttt                                                                                          <  2:241326/140‑1 (MQ=255)

                           gaAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGa                                                                                     >  2:141594/1‑140 (MQ=255)

                           gaAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGa                                                                                     >  1:352929/1‑140 (MQ=255)

                            aaaCAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGAt                                                                                    >  1:140861/1‑140 (MQ=255)

                             aacaacAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATg                                                                                   >  1:328192/1‑140 (MQ=255)

                              acaacaGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATgg                                                                                  >  1:494893/1‑140 (MQ=255)

                              acaacaGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATgg                                                                                  <  2:328987/140‑1 (MQ=255)

                                   aGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAg                                                                             <  2:352929/140‑1 (MQ=255)

                                    gtgtTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAgg                                                                            <  1:141594/140‑1 (MQ=255)

                                    gtgtTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCAGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAgg                                                                            >  1:26739/1‑140 (MQ=255)

                                       ttCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGttt                                                                                          <  1:297975/123‑1 (MQ=255)

                                       ttCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGttt                                                                                          >  2:297975/1‑123 (MQ=255)

                                          gCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCt                                                                      >  2:853731/1‑140 (MQ=255)

                                           cTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTg                                                                     <  2:129963/140‑1 (MQ=255)

                                            tggtggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTgg                                                                    >  2:567028/1‑140 (MQ=255)

                                               tggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTTGGga                                                                 <  2:140861/140‑1 (MQ=255)

                                               tggATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGga                                                                 >  1:282132/1‑140 (MQ=255)

                                                   tGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGaaa                                                             >  2:545712/1‑140 (MQ=255)

                                                    ggCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGaaat                                                            <  2:282132/140‑1 (MQ=255)

                                                    ggCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGaaat                                                            <  1:567028/140‑1 (MQ=255)

                                                              ccTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGAc                                                  <  2:328192/140‑1 (MQ=255)

                                                                     cgcCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGt                                           >  2:460588/1‑140 (MQ=255)

                                                                          aGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAg                                      <  1:460588/140‑1 (MQ=255)

                                                                                      aaCCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAg                          >  2:214772/1‑140 (MQ=255)

                                                                                      aaCCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAg                          >  2:812914/1‑140 (MQ=255)

                                                                                      aaCCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAg                          >  2:734024/1‑140 (MQ=255)

                                                                                           ggTGTTTCCGGGCCGCTGAAAGCCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTAGAAGGTGTTGTCGAAGCTTaa                     >  1:851204/1‑140 (MQ=255)

                                                                                                ttCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACg                >  2:283775/1‑140 (MQ=255)

                                                                                                          cTGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGa      >  1:226014/1‑140 (MQ=255)

                                                                                                           tGAAAGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGAc     <  1:812914/140‑1 (MQ=255)

                                                                                                              aaGTCGGCAACTCGCTGGTGGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGc  <  1:545712/140‑1 (MQ=255)

CTATAAAGATTCCCCTGCCTGTAAAGAGAAACAACAGTGTTCGCTGGTGGATGGCAAAAATACCTTTAGCGCCAAGTATCAACAAGAACCAGGTGTTTCCGGGCCGCTGAAAGTCGGCAACTCGCTGGTAGATGCGTTTACTTTGCAATATTACGAAGGTTTTCCGATGGATCAGGTGGCCTGGGGAGAAATCAAATCTGACCAGCAGTGGAAGGTGTTGTCGAAGCTTAAAAACGGCTACCAGGACAGC  >  NC_000913/1066151‑1066400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A