Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 123 51.0 1731148 99.3% 1719029 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,088,526 T→C A444A (GCA→GCG)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,088,5260TC100.0% 214.3 / NA 58A444A (GCA→GCG) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine N‑deacetylase and beta‑1,6 glycoside hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/28);  total (30/28)

TAAAGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAC  >  NC_000913/1088390‑1088661
                                                                                                                                        |                                                                                                                                       
tAAAGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGct                                                                                                                                      <  2:755492/140‑1 (MQ=255)
 aaaGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGctc                                                                                                                                     <  2:177713/140‑1 (MQ=255)
      cTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGAct                                                                                                                                <  2:563669/140‑1 (MQ=255)
         cttgctGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTg                                                                                                                             <  1:342928/140‑1 (MQ=255)
           tgctGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTc                                                                                                                           <  2:255425/140‑1 (MQ=255)
           tgctGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTc                                                                                                                           <  1:511097/140‑1 (MQ=255)
                 caggCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGGATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGaa                                                                                                                     <  1:525380/137‑1 (MQ=255)
                        tGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGgagag                                                                                                              >  2:681057/1‑140 (MQ=255)
                               ccgcgCACTGGCATCTTCATAATCTGAAAGCAAAGCATAATCGCGGCACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCGAATTGCGATCTGACTCTGTCATCGAAAGAAGAGAGAcctg                                                                                                       >  2:525380/5‑137 (MQ=255)
                                ggTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTg                                                                                                      >  2:322020/1‑140 (MQ=255)
                                  tGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACgg                                                                                                        >  1:243338/1‑136 (MQ=255)
                                  tGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACgg                                                                                                        <  2:243338/136‑1 (MQ=255)
                                        ggCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCATCTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGtt                                                                                              <  2:100803/140‑1 (MQ=255)
                                        ggCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGtt                                                                                              <  2:194788/140‑1 (MQ=255)
                                         gCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTc                                                                                             >  2:106774/1‑140 (MQ=255)
                                                   aaTCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAAtt                                                                                   >  2:346390/1‑140 (MQ=255)
                                                   aaTCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAAtt                                                                                   >  1:217809/1‑140 (MQ=255)
                                                         aaagcaaagcATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCt                                                                             >  1:632050/1‑140 (MQ=255)
                                                         aaagcaaagcATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCt                                                                             >  1:343661/1‑140 (MQ=255)
                                                         aaagcaaagcATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCt                                                                             <  2:632050/140‑1 (MQ=255)
                                                          aagcaaagcaTCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCtt                                                                            >  1:2979/1‑140 (MQ=255)
                                                                agcaTCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTtct                                                                      <  2:469316/140‑1 (MQ=255)
                                                                     catcGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCt                                                                 >  1:657261/1‑140 (MQ=255)
                                                                      atcGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTg                                                                >  1:90879/1‑140 (MQ=255)
                                                                            gAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTa                                                          >  2:1707/1‑140 (MQ=255)
                                                                            gAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTa                                                          <  2:90879/140‑1 (MQ=255)
                                                                            gAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTa                                                          >  2:578064/1‑140 (MQ=255)
                                                                                  tatGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTAtt                                                    >  2:618201/1‑140 (MQ=255)
                                                                                      ccATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTAc                                                <  1:578064/140‑1 (MQ=255)
                                                                                       cATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACt                                               >  1:731180/1‑140 (MQ=255)
                                                                                         tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGTCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcg                                             >  2:559173/1‑140 (MQ=255)
                                                                                         tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTAtttt                                                  >  1:273827/1‑135 (MQ=255)
                                                                                         tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTAtttt                                                  <  2:273827/135‑1 (MQ=255)
                                                                                         tCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcg                                             >  2:641859/1‑140 (MQ=255)
                                                                                          cAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcgc                                            >  1:151368/1‑140 (MQ=255)
                                                                                           aaaaGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcgcg                                           <  2:151368/140‑1 (MQ=255)
                                                                                           aaaaGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTcgcg                                           <  1:681057/140‑1 (MQ=255)
                                                                                                cagcaTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAAt                                      >  2:276530/1‑140 (MQ=255)
                                                                                                 agcaTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAAtg                                     <  1:276530/140‑1 (MQ=255)
                                                                                                  gcaTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAAtgt                                    <  1:340697/140‑1 (MQ=255)
                                                                                                    aTGTCCGGCAAGATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTgg                                  <  2:657261/140‑1 (MQ=255)
                                                                                                          ggCAAGATCTTCATATAACATGCTAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTa                            <  1:618201/140‑1 (MQ=255)
                                                                                                               gATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATcc                       <  1:641859/140‑1 (MQ=255)
                                                                                                               gATCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCACGTTAATGTGGGATCTAAATcc                       <  2:217809/140‑1 (MQ=255)
                                                                                                                 tCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATcc                       >  1:769820/1‑138 (MQ=255)
                                                                                                                 tCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATcc                       <  2:769820/138‑1 (MQ=255)
                                                                                                                 tCTTCATATAACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCa                     >  2:502692/1‑140 (MQ=255)
                                                                                                                        ataACATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAAt              >  1:513273/1‑140 (MQ=255)
                                                                                                                          aaCATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATAc            >  2:152064/1‑140 (MQ=255)
                                                                                                                            cATGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCg          >  1:699389/1‑140 (MQ=255)
                                                                                                                             aTGCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCgg         <  1:152064/140‑1 (MQ=255)
                                                                                                                               gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  2:369293/1‑140 (MQ=255)
                                                                                                                               gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  2:609213/1‑140 (MQ=255)
                                                                                                                               gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  1:709323/1‑140 (MQ=255)
                                                                                                                               gCCAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCa       >  2:681261/1‑140 (MQ=255)
                                                                                                                                ccAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTGAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCAt      <  1:106774/140‑1 (MQ=255)
                                                                                                                                 cAACTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATc     <  1:611822/140‑1 (MQ=255)
                                                                                                                                  aaCTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGTCGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATcc    <  1:559173/140‑1 (MQ=255)
                                                                                                                                    cTTGCGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAc  >  1:858516/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
TAAAGCCTGCTTGCTGATAAGCCGTGATAGCCGGTGCACTGGCATCTTCATAATCTGAAAGCAAAGCATCATCGTGGAACAATATGCCATCAAAAGCAGCATGTCCGGCAAGATCTTCATATAACATGCCAACTTGTGCTCTGACTCTGTCATCGAAAGGAGAGAGACGGTGATATTGTTCAGGATGAATTTGTGCTTTTTTCTCCCCTGTTGGTAAGTATTTTACTCGCGTTAATGTGGGATCTAAATCCCAGCTTAATACCGGCATCCAC  >  NC_000913/1088390‑1088661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A