Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,131,098	A→G	A27A (GCA→GCG)	flgB →	flagellar basal‑body rod protein FlgB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,131,098	0	A	G	100.0%	131.2 / NA	36	A27A (GCA→GCG)	flgB	flagellar basal‑body rod protein FlgB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

CGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACA  >  NC_000913/1130968‑1131232

cgTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATc                                                                                                                               >  2:339408/1‑140 (MQ=255)

   aaaGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGcc                                                                                                                            <  2:233464/140‑1 (MQ=255)

         aTTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGcc                                                                                                                      >  1:397503/1‑140 (MQ=255)

           ttAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGa                                                                                                                    <  1:443759/140‑1 (MQ=255)

              aGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATAc                                                                                                                 <  2:397503/140‑1 (MQ=255)

               gCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATAcc                                                                                                                <  1:817155/140‑1 (MQ=255)

                 tGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATAcccc                                                                                                              <  2:40182/140‑1 (MQ=255)

                 tGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATAcccc                                                                                                              <  2:692555/140‑1 (MQ=255)

                   aTGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTg                                                                                                            >  1:688859/1‑140 (MQ=255)

                           aTTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAg                                                                                                    <  2:688859/140‑1 (MQ=255)

                                                  atGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAg                                                                             >  2:85661/1‑140 (MQ=255)

                                                       cGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAAc                                                                        >  1:790479/1‑140 (MQ=255)

                                                         aTAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCA‑GCGCGCGATATCGATTTTGCCAGTGAACTTa                                                                     >  1:525276/1‑140 (MQ=255)

                                                          tAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTa                                                                     >  2:425528/1‑140 (MQ=255)

                                                          tAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTa                                                                     >  1:622267/1‑140 (MQ=255)

                                                          tAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCA‑GCGCGCGATATCGATTTTGCCAGTGAACTTaa                                                                    <  2:525276/140‑1 (MQ=255)

                                                                       gccTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCa                                                        <  1:164317/140‑1 (MQ=255)

                                                                        ccTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCaa                                                       >  1:202600/1‑140 (MQ=255)

                                                                               ttttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGAc                                                <  1:618497/140‑1 (MQ=255)

                                                                               ttttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGAc                                                <  1:124370/140‑1 (MQ=255)

                                                                                tttCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACg                                               >  1:71020/1‑140 (MQ=255)

                                                                                  tCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATAGCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACggg                                             >  2:601432/1‑140 (MQ=255)

                                                                                   caacaaGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGa                                            <  1:426409/140‑1 (MQ=255)

                                                                                   caacaaGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGa                                            <  1:425528/140‑1 (MQ=255)

                                                                                         gagGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAAc                                      <  2:790479/140‑1 (MQ=255)

                                                                                          agGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAAcc                                     <  1:339408/140‑1 (MQ=255)

                                                                                           ggCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCa                                    >  1:135410/1‑140 (MQ=255)

                                                                                            gcgcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCa                                        <  1:93427/135‑1 (MQ=255)

                                                                                            gcgcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCa                                        >  2:93427/1‑135 (MQ=255)

                                                                                            gcgcTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAg                                   >  1:455710/1‑140 (MQ=255)

                                                                                                  aaTCTGCGCGCCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGt                             <  2:71020/140‑1 (MQ=255)

                                                                                                       gcgcgcCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCAc                        <  2:622267/140‑1 (MQ=255)

                                                                                                         gcgcCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTg                      <  1:42428/140‑1 (MQ=255)

                                                                                                          cgcCCAGCGTCAGGAAGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGa                     >  2:500065/1‑140 (MQ=255)

                                                                                                                        aaGTGCTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACg       <  1:539235/140‑1 (MQ=255)

                                                                                                                             cTGGCGGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAaca  >  2:696348/1‑140 (MQ=255)

CGTAAAGGCATTTAAGCTGATGGCAGAATTTTGATACCTGCGGAGGAGATATGCTCGATAAGCTCGACGCCGCCTTACGTTTTCAACAAGAGGCGCTCAATCTGCGCGCCCAGCGTCAGGAAGTGCTGGCAGCAAACATCGCCAATGCCGATACCCCTGGTTATCAGGCGCGCGATATCGATTTTGCCAGTGAACTTAAAAAAGTCATGCAACGTGGACGGGATGCAACCAGTGTGGTTGCACTGACGATGACCTCAACGCAACA  >  NC_000913/1130968‑1131232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A