Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,227,278	A→G	S65S (AGT→AGC)	pliG ←	inhibitor of g‑type lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,227,278	0	A	G	100.0%	157.6 / NA	43	S65S (AGT→AGC)	pliG	inhibitor of g‑type lysozyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/19); total (24/19)

CTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAG  >  NC_000913/1227142‑1227413

cTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTc                                                                                                                                      >  1:725145/1‑140 (MQ=255)

   gCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTcaca                                                                                                                                   >  1:710690/1‑140 (MQ=255)

          atatTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACtt                                                                                                                            >  1:257550/1‑140 (MQ=255)

                          gggAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCtttttggca                                                                                                            >  2:628862/1‑136 (MQ=255)

                           ggAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATTAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCa                                                                                                           <  2:409746/140‑1 (MQ=255)

                             agtgagtATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACC‑TTTTTGGCATAg                                                                                                        <  1:628862/140‑1 (MQ=255)

                              gtgagtATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAg                                                                                                        <  1:688993/140‑1 (MQ=255)

                              gtgagtATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAg                                                                                                        <  1:281876/140‑1 (MQ=255)

                               tgagtATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGa                                                                                                       <  2:725145/140‑1 (MQ=255)

                                 agtATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGa                                                                                                       <  2:548549/138‑1 (MQ=255)

                                 agtATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGa                                                                                                       >  1:548549/1‑138 (MQ=255)

                                        tCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATg                                                                                              >  1:771159/1‑140 (MQ=255)

                                         ccGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTatat                                                                                               >  1:174051/1‑138 (MQ=255)

                                         ccGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTatat                                                                                               <  2:174051/138‑1 (MQ=255)

                                            tGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTatc                                                                                          >  1:397509/1‑140 (MQ=255)

                                                          tGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATCAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCt                                                                            >  1:553886/1‑140 (MQ=255)

                                                            gggAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCttt                                                                          >  2:466327/1‑140 (MQ=255)

                                                                atatCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTAttt                                                                      <  1:304217/140‑1 (MQ=255)

                                                                atatCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTAttt                                                                      <  2:771159/140‑1 (MQ=255)

                                                                atatCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTAttt                                                                      <  1:105518/140‑1 (MQ=255)

                                                                            aTCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACt                                                          >  1:55572/1‑140 (MQ=255)

                                                                            aTCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACt                                                          >  1:658232/1‑140 (MQ=255)

                                                                            aTCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTCATTTCGCCGGAGTACt                                                          >  1:624087/1‑140 (MQ=255)

                                                                               aaCTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAg                                                       <  2:397509/140‑1 (MQ=255)

                                                                                aCTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAgc                                                      <  2:710690/140‑1 (MQ=255)

                                                                                 cTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCGCTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAgcg                                                     <  2:658232/140‑1 (MQ=255)

                                                                                 cTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTCATTTCGCCGGAGTACTGAgcg                                                     <  2:624087/140‑1 (MQ=255)

                                                                                      tcatcGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCgctgct                                                >  2:40684/1‑140 (MQ=255)

                                                                                          cGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATgg                                            >  1:492055/1‑140 (MQ=255)

                                                                                           gATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGc                                           <  2:492055/140‑1 (MQ=255)

                                                                                              tCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCtt                                        >  1:279978/1‑140 (MQ=255)

                                                                                                 tGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGT‑ATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCt                                    >  2:78529/1‑140 (MQ=255)

                                                                                                      cGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGT‑ATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACt                               <  1:78529/140‑1 (MQ=255)

                                                                                                        aaGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTc                              <  2:369304/140‑1 (MQ=255)

                                                                                                        aaGAGGTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTc                              >  1:369304/1‑140 (MQ=255)

                                                                                                             gTAAGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACAt                         >  1:520969/1‑140 (MQ=255)

                                                                                                                aGTATCAGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGa                      <  1:466327/140‑1 (MQ=255)

                                                                                                                      aGCGCCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCt                >  2:594766/1‑140 (MQ=255)

                                                                                                                       gcgcCTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCtt               >  2:392627/1‑140 (MQ=255)

                                                                                                                          ccTTCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTAcc            >  1:125226/1‑140 (MQ=255)

                                                                                                                            ttCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCtt               <  2:226481/135‑1 (MQ=255)

                                                                                                                            ttCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCtt               >  1:226481/1‑135 (MQ=255)

                                                                                                                            ttCATTAGAGATGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCg          >  1:282947/1‑140 (MQ=255)

                                                                                                                                    agaTGCTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAg  >  1:663336/1‑140 (MQ=255)

CTCGCAGCTCATATTTGCCAGAAGCAGGGAGTGAGTATTGTCCGTGACTATCGAGTTCTGGGGAATATCTGGATAAATCAACTGAATCATCGATTCCTGGCCCGAAGAGGTAAGTATCAGCGCCTTCATTAGAGATACTCACATGCACTTTCTGACCTTTTTTGGCATAGAAGGTATATGTATCGTAATCGTATCCCTTTATTTCGCCGGAGTACTGAGCGCTGCTATGGCCTTTTCTGAACTCAACATTGACATTCTTACCCGCAGCAAAG  >  NC_000913/1227142‑1227413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A