Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,599,375	G→A	Q279* (CAA→TAA)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,599,375	0	G	A	100.0%	113.2 / NA	33	Q279* (CAA→TAA)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/14); total (19/14)

GCATCGCGGAACCAGCGCATGGTGAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTT  >  NC_000913/1599239‑1599509

gCATCGCGGAACCAGCGCATGGTGAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTtactg                                                                                                                                     <  1:613583/140‑1 (MQ=255)

     gcgGAACCAGCGCATGGTGAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGa                                                                                                                                >  1:437847/1‑140 (MQ=255)

               cgcATGGTGAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTgccgcc                                                                                                                      >  1:431462/1‑140 (MQ=255)

                   tGGTGAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAAATTTACAACTTACTGCCAGAATGTGCCGCCAAg                                                                                                                   >  2:359880/1‑140 (MQ=255)

                       gAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACcg                                                                                                              <  2:437847/140‑1 (MQ=255)

                            cGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCTAAAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGt                                                                                                          <  1:359880/140‑1 (MQ=255)

                              gTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTtg                                                                                                       >  1:621528/1‑140 (MQ=255)

                                                     ttGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAAcc                                                                                >  2:817727/1‑140 (MQ=255)

                                                        tACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCaa                                                                             >  1:412029/1‑140 (MQ=255)

                                                          ccATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATATCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAgg                                                                           <  1:741280/140‑1 (MQ=255)

                                                             tGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCaa                                                                        >  2:803125/1‑140 (MQ=255)

                                                             tGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCaa                                                                        <  2:621528/140‑1 (MQ=255)

                                                             tGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCaa                                                                        >  2:562781/1‑140 (MQ=255)

                                                             tGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCaa                                                                        >  2:186086/1‑140 (MQ=255)

                                                              gCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGc                                                                                      <  2:840453/125‑1 (MQ=255)

                                                              gCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGc                                                                                      >  1:840453/1‑125 (MQ=255)

                                                               ccAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAAcc                                                                      <  1:858963/140‑1 (MQ=255)

                                                               ccAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAAcc                                                                      <  1:186086/140‑1 (MQ=255)

                                                               ccAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAAcc                                                                      <  1:803125/140‑1 (MQ=255)

                                                                         cATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACg                                                            >  2:552775/1‑140 (MQ=255)

                                                                            gAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCg                                                         <  1:817727/140‑1 (MQ=255)

                                                                              ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGcc                                                       >  2:254693/1‑140 (MQ=255)

                                                                              ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGcc                                                       >  2:257794/1‑140 (MQ=255)

                                                                              ggATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGcc                                                       <  1:48211/140‑1 (MQ=255)

                                                                               gATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGcct                                                      >  1:618696/1‑140 (MQ=255)

                                                                                    aCGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCtcctcc                                                 >  2:579702/1‑140 (MQ=255)

                                                                                     cgcgCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCa                                                <  1:552775/140‑1 (MQ=255)

                                                                                                      gggTCTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGcc                               <  2:618696/140‑1 (MQ=255)

                                                                                                          cTGTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGcc                           >  1:444491/1‑140 (MQ=255)

                                                                                                            gTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGcc                               >  1:61479/1‑134 (MQ=255)

                                                                                                            gTCACCGGCGCGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGcc                               <  2:61479/134‑1 (MQ=255)

                                                                                                                   gcgcGGCT‑AAATTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCg                  >  1:659883/1‑140 (MQ=255)

                                                                                                                            aaaTTTACAACTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTc          <  2:444491/140‑1 (MQ=255)

                                                                                                                                    aaCTTACTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCtt  >  2:270556/1‑140 (MQ=255)

GCATCGCGGAACCAGCGCATGGTGAGTCCGGTAAAAAAGCTTATAGATTCAGCTTGTACCATGCCAGGAATAACATGAGGATTAACGCGCACGTTCATTTCTGGGTCTGTCACCGGCGCGGCT‑AAATTTACAACTTGCTGCCAGAATGTGCCGCCAAGAACCGCGGTTTGTGCCGGACGCACAACGCCTAACCCAAGGCAACCAAGCTGCACGTCGCCTCCTCCAACGACCACCGGAGTGCCCGCCTTCAGACCGCAGAGTTCCGCCGCTT  >  NC_000913/1599239‑1599509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A