Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,606,759	A→G	V220V (GTA→GTG)	lsrF →	3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,606,759	0	A	G	100.0%	133.2 / NA	37	V220V (GTA→GTG)	lsrF	3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/22); total (15/22)

GGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCG  >  NC_000913/1606622‑1606891

ggCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGcc                                                                                                                                    <  2:435720/140‑1 (MQ=255)

 gCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGccc                                                                                                                                   <  2:213859/140‑1 (MQ=255)

 gCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGccc                                                                                                                                   >  2:178880/1‑140 (MQ=255)

  cGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCa                                                                                                                                  >  1:587122/1‑140 (MQ=255)

  cGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCCAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCa                                                                                                                                  <  2:556511/140‑1 (MQ=255)

      ggCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGt                                                                                                                              >  1:366768/1‑140 (MQ=255)

           aGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTg                                                                                                                         >  1:72350/1‑140 (MQ=255)

            gATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGc                                                                                                                        <  1:706235/140‑1 (MQ=255)

                     cgcgATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTaa                                                                                                               >  1:624906/1‑140 (MQ=255)

                      gcgATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGCaaa                                                                                                              >  1:603692/1‑140 (MQ=255)

                                      ctcgctcgCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGcgcg                                                                                              <  2:624906/140‑1 (MQ=255)

                                       tcgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGa                                                                                             <  2:864424/140‑1 (MQ=255)

                                       tcgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGa                                                                                             <  2:643582/140‑1 (MQ=255)

                                        cgctcgcGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAg                                                                                            >  1:818621/1‑140 (MQ=255)

                                                  tCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGaaa                                                                                  >  1:446624/1‑140 (MQ=255)

                                                     aaTCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAAtgt                                                                               >  1:800462/1‑140 (MQ=255)

                                                           cgcTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGc                                                                         >  2:662135/1‑140 (MQ=255)

                                                                taaTTGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGAATGTCTGGTGCCCAGTGTTATTGCTTGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCt                                                                    <  2:458251/139‑1 (MQ=255)

                                                                     ggggCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGa                                                               >  2:437829/1‑140 (MQ=255)

                                                                           cAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAggg                                                         <  1:666909/140‑1 (MQ=255)

                                                                           cAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAggg                                                         <  2:72350/140‑1 (MQ=255)

                                                                           cAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAggg                                                         <  2:603692/140‑1 (MQ=255)

                                                                               ttATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCt                                                     <  2:800462/140‑1 (MQ=255)

                                                                                    aaaaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTgg                                                <  2:398728/140‑1 (MQ=255)

                                                                                      aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg                                              <  2:311543/140‑1 (MQ=255)

                                                                                      aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg                                              >  1:144239/1‑140 (MQ=255)

                                                                                      aaCCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGgtg                                              <  1:282770/140‑1 (MQ=255)

                                                                                           attatGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGat                                         >  1:102140/1‑140 (MQ=255)

                                                                                                  cGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGc                                  <  2:782791/140‑1 (MQ=255)

                                                                                                  cGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGc                                  >  2:104625/1‑140 (MQ=255)

                                                                                                        aGGTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAata                            <  1:145029/140‑1 (MQ=255)

                                                                                                          gTTTTGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATAtt                          >  1:417172/1‑140 (MQ=255)

                                                                                                           ttttGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATAttt                         <  2:144239/140‑1 (MQ=255)

                                                                                                            tttGAACGGATTGTTGCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATAtttt                        <  2:366768/140‑1 (MQ=255)

                                                                                                                          tgCCGGATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATc          <  1:662135/140‑1 (MQ=255)

                                                                                                                               gATGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTg     <  1:437829/140‑1 (MQ=255)

                                                                                                                                aTGTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTgg    <  2:587122/140‑1 (MQ=255)

                                                                                                                                  gTCCGGTGCCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCg  <  2:446624/140‑1 (MQ=255)

GGCGTGGGCAAAGATATGGTGCGCGATCAGCGTTATTTCTCGCTCGCGACTCGAATCGCCGCTGAAATGGGGGCGCAAATTATCAAAACCTATTATGTCGAAAAAGGTTTTGAACGGATTGTTGCCGGATGTCCGGTACCCATTGTTATTGCTGGCGGTAAAAAATTACCGGAGCGCGAGGCGCTGGAAATGTGCTGGCAGGCTATCGATCAGGGCGCTTCTGGTGTGGATATGGGGCGTAATATTTTCCAGTCTGACCATCCGGTGGCG  >  NC_000913/1606622‑1606891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A