Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 123 51.0 1731148 99.3% 1719029 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,868,951 (G)6→5 intergenic (+109/‑4) yeaG → / → yeaH protein kinase YeaG/DUF444 domain‑containing protein YeaH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,868,9460G.100.0% 195.7 / NA 46intergenic (+104/‑9)yeaG/yeaHprotein kinase YeaG/DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (25/21);  total (25/21)

CGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTT  >  NC_000913/1868809‑1869078
                                                                                                                                         |                                                                                                                                    
cGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                                                                                    <  1:241017/140‑1 (MQ=255)
cGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                                                                                    >  1:294698/1‑140 (MQ=255)
          ttGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGAc                                                                                                                         <  1:225664/140‑1 (MQ=255)
               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACATATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGGATACAGTTGGCAAATGTAGTACggg                                                                                                                                    >  2:763983/1‑125 (MQ=255)
               cgtacgtaAATCGTCTTAACAACCCTGGCCCGGTACATATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACggg                                                                                                                                    <  1:763983/125‑1 (MQ=255)
                 tacgtaAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGttt                                                                                                                  >  1:43374/1‑140 (MQ=255)
                       aaTCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGAc                                                                                                            <  2:615239/140‑1 (MQ=255)
                            tCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCg                                                                                                       >  1:614811/1‑140 (MQ=255)
                                      ccTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGc                                                                                             >  1:849866/1‑140 (MQ=255)
                                          gCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCaaaa                                                                                         >  1:292140/1‑140 (MQ=255)
                                              ggTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaa                                                                                     <  2:43374/140‑1 (MQ=255)
                                               gTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa                                                                                    >  1:519800/1‑140 (MQ=255)
                                               gTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaa                                                                                    >  2:668934/1‑140 (MQ=255)
                                                tACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACaaaa                                                                                   >  2:310679/1‑140 (MQ=255)
                                                          aCCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGaa                                                                         >  2:69311/1‑140 (MQ=255)
                                                              ggCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGc                                                                     >  2:62094/1‑140 (MQ=255)
                                                               gCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGcc                                                                    >  2:663083/1‑140 (MQ=255)
                                                               gCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGcc                                                                    >  2:851116/1‑140 (MQ=255)
                                                                  tACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGc                                                                 >  2:701455/1‑140 (MQ=255)
                                                                         aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttt                                                          >  2:109216/1‑140 (MQ=255)
                                                                         aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGtttttt                                                          >  1:841165/1‑140 (MQ=255)
                                                                          cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTa                                                         <  2:614811/140‑1 (MQ=255)
                                                                          cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTa                                                         <  2:466548/140‑1 (MQ=255)
                                                                          cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTa                                                         <  2:519800/140‑1 (MQ=255)
                                                                            gCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACg                                                       >  2:169324/1‑140 (MQ=255)
                                                                            gCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACg                                                       <  2:292140/140‑1 (MQ=255)
                                                                             cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGc                                                      >  1:800009/1‑140 (MQ=255)
                                                                              gAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGcc                                                     >  1:389507/1‑140 (MQ=255)
                                                                                 ccGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTa                                                         <  1:747499/133‑1 (MQ=255)
                                                                                 ccGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTa                                                         >  2:747499/1‑133 (MQ=255)
                                                                                     gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATaa                                              >  1:643169/1‑140 (MQ=255)
                                                                                        ccTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAgc                                           <  1:109216/140‑1 (MQ=255)
                                                                                        ccTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAgc                                           <  1:767118/140‑1 (MQ=255)
                                                                                        ccTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAgc                                           <  1:69311/140‑1 (MQ=255)
                                                                                        ccTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAgc                                           <  2:389507/140‑1 (MQ=255)
                                                                                            agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCaa                                       >  2:167086/1‑140 (MQ=255)
                                                                                                 cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTaa                                  >  2:283073/1‑140 (MQ=255)
                                                                                                  ggCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTaaa                                 <  2:643169/140‑1 (MQ=255)
                                                                                                    cAACACAGGCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACa                               <  1:701455/140‑1 (MQ=255)
                                                                                                            gCGTAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAG‑‑‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                    >  1:421475/1‑140 (MQ=255)
                                                                                                               tAGCATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAG‑‑‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 <  2:421475/140‑1 (MQ=255)
                                                                                                                  cATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 <  2:294698/140‑1 (MQ=255)
                                                                                                                  cATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 >  2:645496/1‑140 (MQ=255)
                                                                                                                  cATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 <  1:663083/140‑1 (MQ=255)
                                                                                                                  cATACAGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 <  2:800009/140‑1 (MQ=255)
                                                                                                                       aGTTGGCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt            >  1:175534/1‑140 (MQ=255)
                                                                                                                           ggCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 <  2:275958/131‑1 (MQ=255)
                                                                                                                           ggCAAATGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                 >  1:275958/1‑131 (MQ=255)
                                                                                                                                 tGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGtt  <  1:668934/140‑1 (MQ=255)
                                                                                                                                 tGTAGTAC‑GGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGtt  <  1:645496/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
CGAATGGTATTTGCGCGTACGTAAATCGTCTTAACAACCCTGGCCCGGTACACATGTTACCGGGCCTACAACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGCAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTT  >  NC_000913/1868809‑1869078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A