Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,870,127	T→C	H391H (CAT→CAC)	yeaH →	DUF444 domain‑containing protein YeaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,870,127	0	T	C	100.0%	119.2 / NA	33	H391H (CAT→CAC)	yeaH	DUF444 domain‑containing protein YeaH
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/17); total (16/17)

ACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCG  >  NC_000913/1869991‑1870261

aCGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTg                                                                                                                                     <  2:293707/140‑1 (MQ=255)

       tAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTa                                                                                                                              <  1:700493/140‑1 (MQ=255)

       tAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTa                                                                                                                              <  1:57234/140‑1 (MQ=255)

              gCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGa                                                                                                                       <  2:472551/140‑1 (MQ=255)

                      ctctCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTg                                                                                                               <  2:50834/140‑1 (MQ=255)

                               ttGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATgcagc                                                                                                      >  2:783625/1‑140 (MQ=255)

                                tGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATgcagca                                                                                                     >  2:511386/1‑140 (MQ=255)

                                          tCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGAc                                                                                           <  1:511386/140‑1 (MQ=255)

                                           ccTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGAcc                                                                                          <  1:571343/140‑1 (MQ=255)

                                              ggCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAgg                                                                                       >  1:624527/1‑140 (MQ=255)

                                               gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                                                                                      <  1:712156/140‑1 (MQ=255)

                                               gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATAACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                                                                                      <  1:219995/140‑1 (MQ=255)

                                               gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATAACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga                                                                                      >  2:219995/1‑140 (MQ=255)

                                                                   tgttCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTcc                                                                  >  1:553032/1‑140 (MQ=255)

                                                                   tgttCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTcc                                                                  >  2:248742/1‑140 (MQ=255)

                                                                          tattaCAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACt                                                           >  2:347356/1‑140 (MQ=255)

                                                                            ttaCAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGt                                                         >  1:662342/1‑140 (MQ=255)

                                                                               cAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTc                                                      >  2:122851/1‑140 (MQ=255)

                                                                                    atatCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGaa                                                             <  1:141524/128‑1 (MQ=255)

                                                                                    atatCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGaa                                                             >  2:141524/1‑128 (MQ=255)

                                                                                    atatCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATaaa                                                 >  1:365477/1‑140 (MQ=255)

                                                                                      atCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACa                                               <  1:122851/140‑1 (MQ=255)

                                                                                                    tcgtGCACATCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTa                                 <  2:553032/140‑1 (MQ=255)

                                                                                                           caTCAGACATTGTGGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGcaa                          <  1:783625/140‑3 (MQ=255)

                                                                                                                     tgtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTAt                   >  2:233158/1‑137 (MQ=255)

                                                                                                                     tgtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTAt                   <  1:233158/137‑1 (MQ=255)

                                                                                                                     tgtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAg                >  2:105757/1‑140 (MQ=255)

                                                                                                                      gtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGc               <  2:365477/140‑1 (MQ=255)

                                                                                                                      gtgGCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGc               <  1:228776/140‑1 (MQ=255)

                                                                                                                        ggCGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCa             >  1:529969/1‑140 (MQ=255)

                                                                                                                          cGAGAATATGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAgg           <  2:624527/140‑1 (MQ=255)

                                                                                                                               atatGAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCAtt      >  2:61600/1‑140 (MQ=255)

                                                                                                                                   gAGCACCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCg  >  1:817906/1‑140 (MQ=255)

ACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTAAAGGCTAAAACTATCAGCCAGGTCATTATCG  >  NC_000913/1869991‑1870261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A