Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,888,830	A→G	V306A (GTT→GCT)	fadD ←	fatty acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,888,830	0	A	G	100.0%	152.8 / NA	42	V306A (GTT→GCT)	fadD	fatty acyl‑CoA synthetase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/21); total (21/21)

TCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAACGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAGCAGGCAGTTAATGGTCAGGGCAA  >  NC_000913/1888697‑1888959

tCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCg                                                                                                                             >  1:123163/1‑140 (MQ=255)

tCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCAT‑CCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGt                                                                                                                            >  1:819953/1‑140 (MQ=255)

           tCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTa                                                                                                                  <  2:462763/140‑1 (MQ=255)

               ccAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACg                                                                                                              >  2:4712/1‑140 (MQ=255)

                    gCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGatat                                                                                                         <  2:123163/140‑1 (MQ=255)

                    gCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGatat                                                                                                         <  1:4712/140‑1 (MQ=255)

                             ccacTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAAc                                                                                                >  2:543503/1‑140 (MQ=255)

                              cacTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAAct                                                                                               >  1:482465/1‑140 (MQ=255)

                               acTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAActc                                                                                              <  1:329863/140‑1 (MQ=255)

                                  tgctgcACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCttt                                                                                           >  1:139394/1‑140 (MQ=255)

                                     tgcACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTAc                                                                                        <  1:374890/140‑1 (MQ=255)

                                        aCTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTAAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCaa                                                                                     <  2:601158/140‑1 (MQ=255)

                                         cTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAAc                                                                                    >  2:63747/1‑140 (MQ=255)

                                               tCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACTAACCCTGGa                                                                              >  2:419935/1‑140 (MQ=255)

                                                  cACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAata                                                                           <  1:543503/140‑1 (MQ=255)

                                                      gCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgc                                                                       >  2:560701/1‑140 (MQ=255)

                                                      gCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgc                                                                       >  1:63614/1‑140 (MQ=255)

                                                       ccTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgcg                                                                      <  1:156219/140‑1 (MQ=255)

                                                        cTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATcgcgc                                                                     <  2:819953/140‑1 (MQ=255)

                                                           cGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCggg                                                                  >  2:174809/1‑140 (MQ=255)

                                                                aGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGt                                                             <  1:419935/140‑1 (MQ=255)

                                                                 gATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTg                                                            <  1:106368/140‑1 (MQ=255)

                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  1:616713/1‑140 (MQ=255)

                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  1:573874/1‑140 (MQ=255)

                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  2:808212/1‑140 (MQ=255)

                                                                            gagaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGtt                                                 >  1:856191/1‑140 (MQ=255)

                                                                              gaAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCt                                               >  1:93988/1‑140 (MQ=255)

                                                                                aaTCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGc                                             <  1:803272/140‑1 (MQ=255)

                                                                                  tCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGccc                                           >  2:745683/1‑140 (MQ=255)

                                                                                   ccAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGccca                                          <  2:139394/140‑1 (MQ=255)

                                                                                          ctgGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGtt                                   >  1:606963/1‑140 (MQ=255)

                                                                                            ggAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCg                                 <  1:786715/140‑1 (MQ=255)

                                                                                            ggAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCg                                 >  1:357284/1‑140 (MQ=255)

                                                                                                       ttGTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAAcagcag                      <  1:745683/140‑1 (MQ=255)

                                                                                                         gTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  2:482465/140‑1 (MQ=255)

                                                                                                         gTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  1:63747/140‑1 (MQ=255)

                                                                                                         gTTCAGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  1:174809/140‑1 (MQ=255)

                                                                                                             aGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    >  2:151621/1‑136 (MQ=255)

                                                                                                             aGCAACGCATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  1:151621/136‑1 (MQ=255)

                                                                                                                    cATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    <  2:121731/129‑1 (MQ=255)

                                                                                                                    cATTGAACAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAgcaggc                    >  1:121731/1‑129 (MQ=255)

                                                                                                                           cAAGGTGTTAGCGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAGCAGGCAGTTAATGGTCAGGGCaa  <  1:808212/140‑1 (MQ=255)

TCCGGTCAGTTTCACCCAACGCTCTGCCACCACTTGCTGCACTGGCATCCCACCGCCTGCGGAAAGATGCAGACTGGAGAAATCCAGCTGCTGGAACTCTTTATTGTTCAGCAACGCATTGAACAAGGTGTTAACGCCCGTGATAGCGGTAAACGGATATTTCGCTAACTCTTTTACCAACCCTGGAATATCGCGCGGGTTAGTGATAAGCAGGTTCTGCCCACCCAGTTCGATAAACAGCAGGCAGTTAATGGTCAGGGCAA  >  NC_000913/1888697‑1888959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A