Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,141,871	A→G	R115R (CGT→CGC)	dcd ←	dCTP deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,141,871	0	A	G	100.0%	98.6 / NA	28	R115R (CGT→CGC)	dcd	dCTP deaminase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/10); total (18/10)

AAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCA  >  NC_000913/2141736‑2142003

aaaGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGcgcg                                                                                                                                  <  1:348334/140‑1 (MQ=255)

  aGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGcc                                                                                                                                <  1:423275/140‑1 (MQ=255)

       aGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGa                                                                                                                           >  1:598015/1‑140 (MQ=255)

              cAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGcgcg                                                                                                                                  <  1:266264/126‑1 (MQ=255)

              cAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGcgcg                                                                                                                                  >  2:266264/1‑126 (MQ=255)

                aTTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGccc                                                                                                                  >  1:762347/1‑140 (MQ=255)

                        gCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTccagcc                                                                                                          >  2:419060/1‑140 (MQ=255)

                                  aGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAg                                                                                                >  1:409202/1‑140 (MQ=255)

                                  aGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAg                                                                                                >  1:752197/1‑140 (MQ=255)

                                     gcCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATc                                                                                             <  2:745545/140‑1 (MQ=255)

                                         gCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCt                                                                                         >  2:289956/1‑140 (MQ=255)

                                                                             tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  1:797831/1‑140 (MQ=255)

                                                                             tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  2:671443/1‑140 (MQ=255)

                                                                             tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  1:801897/1‑140 (MQ=255)

                                                                             tGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgcc                                                     >  1:349182/1‑140 (MQ=255)

                                                                                     gaccagCCCGGATCGATTCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTcc                                             >  2:14584/1‑140 (MQ=255)

                                                                                     gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgccag                                                   <  1:490957/134‑1 (MQ=255)

                                                                                     gaccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACcgccagcgccag                                                   >  2:490957/1‑134 (MQ=255)

                                                                                      accagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCt                                            >  2:249583/1‑140 (MQ=255)

                                                                                       ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                           >  1:435403/1‑140 (MQ=255)

                                                                                       ccagCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTg                                           >  1:368769/1‑140 (MQ=255)

                                                                                          gCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGt                                        <  2:762347/140‑1 (MQ=255)

                                                                                                aTCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGAt                                  >  2:355879/1‑140 (MQ=255)

                                                                                                                       gCACCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCgaga           <  1:419060/140‑1 (MQ=255)

                                                                                                                         aCCATCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAAc         <  1:249583/140‑1 (MQ=255)

                                                                                                                             tCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCg                       >  1:592670/1‑122 (MQ=255)

                                                                                                                             tCAGCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCg                       <  2:592670/122‑1 (MQ=255)

                                                                                                                                gCCCCAGGCGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCa  <  2:598015/140‑1 (MQ=255)

AAAGCTCAGCGCACCAATTAACATGCCCGGACGCAGCGCCAGCGGCAGCTTACCGGAGTTGTAGAACTCCAGCACAATGCAACCAGACCAGCCCGGATCGATGCGGTGCGCGGTGACGTGCACCATCAGCCCCAGACGCGCCAGTGAGGAACGCCCGTCCAGCCAGCCCACCAGATCGGCTGGCAGCGTCACCGACTCCAGCGTCACCGCCAGCGCCAGCTCTCCTGGGTGAAGATAGAACGCCTCGCCCTCGTCGAGAACGATCTCA  >  NC_000913/2141736‑2142003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A