Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,205	G→A	A34V (GCC→GTC)	eutT ←	putative ethanolamine utilization cobalamin adenosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,205	0	G	A	100.0%	101.2 / NA	30	A34V (GCC→GTC)	eutT	putative ethanolamine utilization cobalamin adenosyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/14); total (16/14)

CACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGA  >  NC_000913/2574070‑2574333

caCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACaga                                                                                                                              >  2:211054/1‑140 (MQ=255)

 aCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACagag                                                                                                                             >  1:572305/1‑140 (MQ=255)

   gCCTGCGGATGTTCATCGCTACTGGTAAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAggg                                                                                                                           >  2:375015/1‑140 (MQ=255)

             gTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGa                                                                                                                 <  1:211054/140‑1 (MQ=255)

              ttCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGAc                                                                                                                <  1:675945/140‑1 (MQ=255)

                        cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGa                                                                                                                 >  1:167458/1‑129 (MQ=255)

                        cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGa                                                                                                                 <  2:167458/129‑1 (MQ=255)

                         tGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCa                                                                                                        <  2:561176/137‑1 (MQ=255)

                         tGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCa                                                                                                        >  1:561176/1‑137 (MQ=255)

                            tCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGgaga                                                                                                  <  1:92054/140‑1 (MQ=255)

                                cccATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGa                                                                                              >  1:660764/1‑140 (MQ=255)

                                cccATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGa                                                                                              >  1:757537/1‑140 (MQ=255)

                                     gAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGACCGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGCGAGATGGATctct                                                                                         >  2:785961/1‑140 (MQ=255)

                                        cGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAg                                                                                                       <  1:413488/123‑1 (MQ=255)

                                        cGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAg                                                                                                       >  2:413488/1‑123 (MQ=255)

                                          ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  1:726545/1‑140 (MQ=255)

                                          ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  2:75274/1‑140 (MQ=255)

                                          ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  2:744068/1‑140 (MQ=255)

                                          ggctgcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGcc                                                                                    >  2:686545/1‑140 (MQ=255)

                                              gcggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCg                                                                                <  1:809010/140‑1 (MQ=255)

                                               cggctgcTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGc                                                                               <  1:243812/140‑1 (MQ=255)

                                                           ttCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGt                                                                   <  2:572305/140‑1 (MQ=255)

                                                            tCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt                                                                  <  1:75274/140‑1 (MQ=255)

                                                            tCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt                                                                  <  2:59682/140‑1 (MQ=255)

                                                             cATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTc                                                                 <  2:660764/140‑1 (MQ=255)

                                                                                          tCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATc                                    <  2:757537/140‑1 (MQ=255)

                                                                                                     gATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGa                         >  2:706238/1‑140 (MQ=255)

                                                                                                                        tCCAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAg      >  1:167843/1‑140 (MQ=255)

                                                                                                                          cAGCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGc    >  2:475884/1‑140 (MQ=255)

                                                                                                                            gCAACTCCCGGACAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGa  <  2:726545/140‑1 (MQ=255)

CACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTTCGCTCTTAGCCATGCTTCGGTGATGAAATCTTTCATAGGGATTGCCAGTTAGCCGGCCAGGCGA  >  NC_000913/2574070‑2574333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A