Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,633,034	T→C	D346G (GAC→GGC)	guaB ←	inosine 5'‑monophosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,633,034	0	T	C	100.0%	122.1 / NA	34	D346G (GAC→GGC)	guaB	inosine 5'‑monophosphate dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/18); total (16/18)

ACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATAGAGC  >  NC_000913/2632913‑2633166

aCGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATAc                                                                                                                    >  1:793374/1‑140 (MQ=255)

aCGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATAc                                                                                                                    >  1:707464/1‑140 (MQ=255)

  ggTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCg                                                                                                                  >  2:824618/1‑140 (MQ=255)

          ttGTAAAAACGGCCCGGGTAGAGTCCGTTTTCCCCCGGAGATTCTTCAGTTCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGc                                                                                                          <  2:707464/140‑1 (MQ=255)

              aaGAACGGCCCTGGTAGAGTTCGATTTCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATa                                                                                                      <  1:30936/140‑1 (MQ=255)

                        tgggTAGAGTTCGTTTTCCCCGGGAGTTTTTTCAGTCCCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATAc                                                                                            <  2:611582/138‑1 (MQ=255)

                             agagTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTg                                                                                       <  1:677337/140‑1 (MQ=255)

                                 ttCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGGACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCtt                                                                                   >  2:702351/1‑140 (MQ=255)

                                 ttCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCtt                                                                                   >  2:673074/1‑140 (MQ=255)

                                 ttCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCtt                                                                                   >  1:354111/1‑140 (MQ=255)

                                   cgttttCCCCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTcc                                                                                 <  1:178223/137‑1 (MQ=255)

                                               gagaTTCTTCAGTACCCGCCAGCATGGGACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACt                                                                     <  1:702351/140‑1 (MQ=255)

                                               gagaTTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACt                                                                     <  1:269151/140‑1 (MQ=255)

                                                   ttcttcAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGt                                                                 >  1:747791/1‑140 (MQ=255)

                                                           tACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACa                                                         <  1:824618/140‑1 (MQ=255)

                                                            ccccGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAg                                                        <  1:166896/139‑1 (MQ=255)

                                                                gCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGt                                                    >  1:141347/1‑140 (MQ=255)

                                                                      aTGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTg                                              <  2:793374/140‑1 (MQ=255)

                                                                                    cACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGc                                <  1:620422/140‑1 (MQ=255)

                                                                                    cACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGc                                >  2:184794/1‑140 (MQ=255)

                                                                                      ccGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCa                              >  2:723626/1‑140 (MQ=255)

                                                                                       cgcgCTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAg                             >  2:388359/1‑140 (MQ=255)

                                                                                        gcgcTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGt                            <  2:354111/140‑1 (MQ=255)

                                                                                        gcgcTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGt                            <  1:184794/140‑1 (MQ=255)

                                                                                         cgcTTGCGCCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTc                           >  2:29503/1‑140 (MQ=255)

                                                                                              gcgcCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGAt                      >  2:150128/1‑140 (MQ=255)

                                                                                                gcCAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATAc                    >  1:109667/1‑140 (MQ=255)

                                                                                                  cAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGa                  <  2:310622/140‑1 (MQ=255)

                                                                                                  cAGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGa                  <  1:150128/140‑1 (MQ=255)

                                                                                                   aGCGGCGATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAg                 <  1:723626/140‑1 (MQ=255)

                                                                                                    gcggcgATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGt                >  2:650503/1‑140 (MQ=255)

                                                                                                        cgATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTa            <  1:388359/140‑1 (MQ=255)

                                                                                                        cgATAGCTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTa            <  2:747791/140‑1 (MQ=255)

                                                                                                              cTTTGGCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATa      <  2:218366/140‑1 (MQ=255)

                                                                                                                  ggCGATGCCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATAGAGc  >  2:546384/1‑140 (MQ=255)

ACGGTAAGATTTGTAAGAACGGCCCTGGTAGAGTTCGATTTCACCCGGAGATTCTTCAGTACCCGCCAGCATGGAACCTACCATCACCGCGCTTGCGCCAGCGGCGATAGCTTTGGCGATGTCGCCGGAGAAGCGAATACCGCCATCAGCGATAACCGGAATACCGGTGCCTTCCAGGGCTTCTACTGCGTCAGCAACAGCGGTAATCTGCGGAACACCGACGCCAGTCACGATACGAGTTGTACAGATAGAGC  >  NC_000913/2632913‑2633166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A