Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,675,181	G→A	A194V (GCG→GTG)	yphC ←	putative zinc‑binding dehydrogenase YphC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,675,181	0	G	A	97.7%	142.6 / NA	43	A194V (GCG→GTG)	yphC	putative zinc‑binding dehydrogenase YphC
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (19/23); minor base C (0/1); total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTC  >  NC_000913/2675049‑2675311

tCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGACGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGcat                                                                                                                             >  1:812640/1‑140 (MQ=255)

                  aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCTGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc                                                                                                           <  1:345039/140‑1 (MQ=255)

                  aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc                                                                                                           <  1:171982/140‑1 (MQ=255)

                  aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc                                                                                                           <  2:207211/140‑1 (MQ=255)

                  aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc                                                                                                           <  2:141201/140‑1 (MQ=255)

                  aGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc                                                                                                           <  1:804126/140‑1 (MQ=255)

                  aGACCTTCGGTGGTTGCTAAATAGACGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGAc                                                                                                           <  2:812640/140‑1 (MQ=255)

                        tCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGccc                                                                                                     >  2:542784/1‑140 (MQ=255)

                                 gCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAgaccgac                                                                                            >  1:10295/1‑140 (MQ=255)

                                     aaTAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGaccacc                                                                                        <  1:542784/140‑1 (MQ=255)

                                           cGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACg                                                                                  <  2:680970/140‑1 (MQ=255)

                                               ctCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGACCCCTTTTGCACCGCGACCTTTCCCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTAt                                                                              <  2:177770/139‑1 (MQ=255)

                                               aTCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAgaccgac                                                                                            >  2:61999/1‑126 (MQ=255)

                                               aTCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAgaccgac                                                                                            <  1:61999/126‑1 (MQ=255)

                                               aTCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTAt                                                                              >  1:388920/1‑140 (MQ=255)

                                                  cATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCAc                                                                           >  1:786083/1‑140 (MQ=255)

                                                   aTCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACt                                                                          <  1:408617/140‑1 (MQ=255)

                                                   aTCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACt                                                                          >  2:408617/1‑140 (MQ=255)

                                                    tCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTg                                                                         >  1:209448/1‑140 (MQ=255)

                                                            aaCTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATGATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAAc                                                                 >  2:138074/1‑140 (MQ=255)

                                                            aaCTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAAc                                                                 >  2:310609/1‑140 (MQ=255)

                                                                  tttGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTcgcc                                                           >  2:20612/1‑140 (MQ=255)

                                                                     gCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCcgcg                                                        <  1:778863/140‑1 (MQ=255)

                                                                       cATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCa                                                      <  1:20612/140‑1 (MQ=255)

                                                                         tCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCaaa                                                    >  2:439727/1‑140 (MQ=255)

                                                                          cGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCaaaa                                                   <  2:10295/140‑1 (MQ=255)

                                                                                    tCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGTCCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATa                                         <  2:786083/140‑1 (MQ=255)

                                                                                    tCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATa                                         <  2:209448/140‑1 (MQ=255)

                                                                                    tCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATa                                         <  2:319719/140‑1 (MQ=255)

                                                                                             atatCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTAcc                                <  2:70024/140‑1 (MQ=255)

                                                                                              tatCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCa                               >  2:360791/1‑140 (MQ=255)

                                                                                                    cGCCGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAAcgccg                         <  2:291187/140‑1 (MQ=255)

                                                                                                      ccGATGATCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCa                       >  2:150060/1‑140 (MQ=255)

                                                                                                             tCCGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATa                <  1:310609/140‑1 (MQ=255)

                                                                                                               cGTTTTGCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATaaa              >  2:132709/1‑140 (MQ=255)

                                                                                                                     gCACCGCGACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCAcc        <  2:388920/140‑1 (MQ=255)

                                                                                                                         cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt      >  2:303174/1‑138 (MQ=255)

                                                                                                                         cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt      >  2:370642/1‑138 (MQ=255)

                                                                                                                         cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt      <  1:211788/138‑1 (MQ=255)

                                                                                                                         cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt      >  2:211788/1‑138 (MQ=255)

                                                                                                                         cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt      <  1:370642/138‑1 (MQ=255)

                                                                                                                         cgcgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCAt      <  1:303174/138‑1 (MQ=255)

                                                                                                                           cgACCTTTCACCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTc  >  1:352220/1‑140 (MQ=255)

TCGGCGATAATCTGCGGCAGACCTTCGGTGGTTGCTAAATAGCCGTGATCCATCACCCCTAACTGTTTTGCCATCGCCAGACGTTCCGGCAGCATATCAACGCCGATGATCCGTTTTGCACCGCGACCTTTCGCCAGCATCATCGCCATCATGCCGACTGGCCCCAGACCGACCACCAGCACGTTATCACTGCCGGAAACTTCGCCGCGCAAAATTCCTTCATACGCTGTACCAACGCCGCAACTGATAAACGCACCATCTTC  >  NC_000913/2675049‑2675311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A