Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,000,775	A→G	N144S (AAC→AGC)	xdhA →	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,000,775	0	A	G	100.0%	198.0 / NA	54	N144S (AAC→AGC)	xdhA	putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/28); total (26/28)

GCCGTTGCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGA  >  NC_000913/3000639‑3000898

gCCGTTGCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCgg                                                                                                                          >  2:162383/1‑140 (MQ=255)

 ccGTTGCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGt                                                                                                                         >  2:755750/1‑140 (MQ=255)

    ttGCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGc                                                                                                                      >  2:468171/1‑140 (MQ=255)

      gCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCaa                                                                                                                    <  2:157318/140‑1 (MQ=255)

      gCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCaa                                                                                                                    <  2:525056/140‑1 (MQ=255)

        cATCGTCGTGGCGCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTAGCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACGAATCCATAGCGGTGGCAAtt                                                                                                                  <  1:573818/140‑1 (MQ=255)

                  gCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACa                                                                                                        <  1:755517/140‑1 (MQ=255)

                      gcgATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGc                                                                                                    >  2:109267/1‑140 (MQ=255)

                       cgATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCa                                                                                                   >  1:594763/1‑140 (MQ=255)

                       cgATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAAACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCa                                                                                                   >  1:136196/1‑140 (MQ=255)

                            aaCTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATg                                                                                              <  1:162383/140‑1 (MQ=255)

                            aaCTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATg                                                                                              <  1:528784/140‑1 (MQ=255)

                               tCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGTCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCg                                                                                           <  1:755750/140‑1 (MQ=255)

                                      aGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTa                                                                                    >  1:121819/1‑140 (MQ=255)

                                        aaaaaGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAAt                                                                                  <  1:109267/140‑1 (MQ=255)

                                         aaaaGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATg                                                                                 <  2:121819/140‑1 (MQ=255)

                                           aaGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTa                                                                                    <  2:282972/135‑1 (MQ=255)

                                           aaGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTa                                                                                    >  1:282972/1‑135 (MQ=255)

                                              cggcgCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCcaa                                                                            <  1:850122/140‑1 (MQ=255)

                                               ggcgCAATTGGTCAGCATTCAGTGGCAAGAATTACCCGTTATCGCCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCcaac                                                                           >  2:806868/1‑140 (MQ=255)

                                                        ggTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCg                                                                  >  1:113816/1‑140 (MQ=255)

                                                           cAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATg                                                               >  1:360356/1‑140 (MQ=255)

                                                                ttGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATgccgcc                                                          <  1:644956/140‑1 (MQ=255)

                                                                  gAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGa                                                        <  1:292956/140‑1 (MQ=255)

                                                                   aGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTCCTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGAc                                                       >  1:64058/1‑140 (MQ=255)

                                                                   aGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGAc                                                       >  1:244546/1‑140 (MQ=255)

                                                                   aGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGAc                                                       >  2:444463/1‑140 (MQ=255)

                                                                       gCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTAcc                                                   <  1:444463/140‑1 (MQ=255)

                                                                        cAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCa                                                  >  2:454232/1‑140 (MQ=255)

                                                                        cAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCa                                                  >  1:853014/1‑140 (MQ=255)

                                                                        cAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCa                                                  >  2:171668/1‑140 (MQ=255)

                                                                             aTTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTAc                                             <  2:853014/140‑1 (MQ=255)

                                                                              ttACCCGTTATCGCCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACa                                            <  1:806868/140‑1 (MQ=255)

                                                                                 cccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAggg                                         >  2:199202/1‑140 (MQ=255)

                                                                                 cccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAggg                                         >  2:473553/1‑140 (MQ=255)

                                                                                  ccGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAgggg                                        <  1:171668/140‑1 (MQ=255)

                                                                                   cGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCTATGCCGCCGACTACCAGGTACAGGGGc                                       <  2:594763/140‑1 (MQ=255)

                                                                                   cGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGc                                       <  2:244546/140‑1 (MQ=255)

                                                                                      tATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACt                                    >  1:576216/1‑140 (MQ=255)

                                                                                           ccacGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAg                               >  1:446230/1‑140 (MQ=255)

                                                                                             acGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGAc                             <  1:95642/140‑1 (MQ=255)

                                                                                              cGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGAcc                            >  2:520075/1‑140 (MQ=255)

                                                                                               gCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGAccc                           >  1:581072/1‑140 (MQ=255)

                                                                                                ccAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGAcccc                          >  2:93876/1‑140 (MQ=255)

                                                                                                   gAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGAc                             >  2:569337/1‑134 (MQ=255)

                                                                                                   gAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGAc                             <  1:569337/134‑1 (MQ=255)

                                                                                                    aaGCGGCGCTGGCAGAAGACGCTGCACC‑ATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCa                                      >  2:396276/1‑123 (MQ=255)

                                                                                                    aaGCGGCGCTGGCAGAAGACGCTGCACC‑ATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCa                                      <  1:396276/123‑1 (MQ=255)

                                                                                                     aGCGGCGCTGGCAGAAGACGCTGCATCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTa                     <  1:113409/140‑1 (MQ=255)

                                                                                                     aGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTa                     <  2:587554/140‑1 (MQ=255)

                                                                                                              ggCAGAAGACGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACAtt            <  1:454232/140‑1 (MQ=255)

                                                                                                                  gaaaaCGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTca        <  2:136196/140‑1 (MQ=255)

                                                                                                                   aagaCGCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTcac       <  1:473553/140‑1 (MQ=255)

                                                                                                                        gCTGCACCAATCCATAGCGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGa  <  2:113816/140‑1 (MQ=255)

GCCGTTGCCATCGTCGTGGCCCGCGATGAACTCACGGCAGAAAAAGCGGCGCAATTGGTCAGCATTGAGTGGCAAGAATTACCCGTTATCACCACGCCAGAAGCGGCGCTGGCAGAAGACGCTGCACCAATCCATAACGGTGGCAATTTACTGAAACAAAGCACGATGTCGACGGGTAATGTCCAACAAACAATCGATGCCGCCGACTACCAGGTACAGGGGCACTATCAGACCCCCGTTATTCAACATTGTCACATGGA  >  NC_000913/3000639‑3000898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A