Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,103,886	C→T	H292Y (CAT→TAT)	mutY →	adenine DNA glycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,103,886	0	C	T	100.0%	91.3 / NA	28	H292Y (CAT→TAT)	mutY	adenine DNA glycosylase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (13/15); total (13/15)

GCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCT  >  NC_000913/3103753‑3104010

gcAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCt                                                                                                                        <  2:737382/140‑1 (MQ=255)

    cGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAg                                                                                                                    >  2:865461/1‑140 (MQ=255)

        cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCAt                                                                                                                >  2:804087/1‑140 (MQ=255)

        cgccgAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCAt                                                                                                                <  1:648289/140‑1 (MQ=255)

                  aTTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAg                                                                                                      <  2:230213/140‑1 (MQ=255)

                    tgtgGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGat                                                                                                    <  1:114390/140‑1 (MQ=255)

                         ggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATtgt                                                                                               <  1:865461/140‑1 (MQ=255)

                         ggCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATtgt                                                                                               >  2:684611/1‑140 (MQ=255)

                              tttATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTa                                                                                          <  1:804087/140‑1 (MQ=255)

                                       tttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTc                                                                                 >  2:303744/1‑140 (MQ=255)

                                       tttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTc                                                                                 >  2:398416/1‑140 (MQ=255)

                                        ttCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTcc                                                                                >  2:17009/1‑140 (MQ=255)

                                         tCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTccc                                                                               >  2:67158/1‑140 (MQ=255)

                                            cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                            >  1:613292/1‑140 (MQ=255)

                                            cGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCgtg                                                                            >  2:85652/1‑140 (MQ=255)

                                                 tttGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTgtcgtc                                                                       >  2:564940/1‑140 (MQ=255)

                                                          gaagaaAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCgg                                                              <  1:684611/140‑1 (MQ=255)

                                                               aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCa                                                         <  1:398416/140‑1 (MQ=255)

                                                               aaGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCa                                                         <  2:613292/140‑1 (MQ=255)

                                                                  tttGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATgg                                                      >  1:19585/1‑140 (MQ=255)

                                                                    tGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGAt                                                    <  1:85652/140‑1 (MQ=255)

                                                                              gctggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATg                                          <  1:303744/140‑1 (MQ=255)

                                                                                 ggcGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATgcgc                                       <  1:564940/140‑1 (MQ=255)

                                                                                   cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGctc                                     <  2:347573/140‑1 (MQ=255)

                                                                                   cgcAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGctc                                     <  1:67158/140‑1 (MQ=255)

                                                                                        cGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGta                                >  2:287577/1‑140 (MQ=255)

                                                                                        cGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGta                                <  1:17009/140‑1 (MQ=255)

                                                                                                   tgcCGATAACCTGACGCAACTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAgcgc                     >  1:335930/1‑140 (MQ=255)

                                                                                                                      cTGACCGCGTTTCGGTATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCt  >  1:293284/1‑140 (MQ=255)

GCAGCGTCCGCCGAGCGGATTGTGGGGCGGTTTATACTGTTTCCCGCAGTTTGCCGACGAAGAAAGTTTGCGGCAGTGGCTGGCGCAACGGCAGATTGCTGCCGATAACCTGACGCAACTGACCGCGTTTCGGCATACCTTCAGCCATTTCCACTTAGATATTGTGCCTATGTGGCTTCCCGTGTCGTCATTCACCGGCTGCATGGATGAAGGCAATGCGCTCTGGTATAACTTAGCGCAACCGCCGTCAGTTGGCCT  >  NC_000913/3103753‑3104010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A