Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,279,899	A→G	E329G (GAA→GGA)	kbaZ →	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,279,899	0	A	G	100.0%	130.7 / NA	37	E329G (GAA→GGA)	kbaZ	tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/20); total (17/20)

TGGCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTG  >  NC_000913/3279768‑3280035

tGGCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGc                                                                                                                                  >  2:277941/1‑140 (MQ=255)

 ggCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCa                                                                                                                                 <  2:598317/140‑1 (MQ=255)

 ggCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCa                                                                                                                                 >  2:466705/1‑140 (MQ=255)

     cAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGc                                                                                                                             <  1:636172/140‑1 (MQ=255)

      aGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGcc                                                                                                                            <  2:534971/140‑1 (MQ=255)

         gcTGACCTTCGCCCTGCGTTAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACt                                                                                                                         >  2:3937/1‑140 (MQ=255)

             aCCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCa                                                                                                                     >  2:480586/1‑140 (MQ=255)

                 tCGCCCTGCGTTAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGt                                                                                                                 <  1:3937/140‑1 (MQ=255)

                         cGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCaa                                                                                                         >  2:160258/1‑140 (MQ=255)

                         cGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCaa                                                                                                         >  1:721670/1‑140 (MQ=255)

                         cGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTAGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCaa                                                                                                         >  2:285229/1‑140 (MQ=255)

                              aGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAAcgcgc                                                                                                    <  1:391894/140‑1 (MQ=255)

                                          tctGGCGGCGATTGAAGAAGAGCGGGTGCCAGCGAAAGCCTGCTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACAGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        <  2:593589/140‑1 (MQ=255)

                                          tctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        <  2:49526/140‑1 (MQ=255)

                                          tctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        <  1:496046/140‑1 (MQ=255)

                                          tctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGgcgc                                                                                        >  1:126115/1‑140 (MQ=255)

                                           ctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGcgcg                                                                                       <  1:690142/140‑1 (MQ=255)

                                           ctGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGcgcg                                                                                       <  1:733566/140‑1 (MQ=255)

                                              gcggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTATGGCGCGTgg                                                                                    >  1:593589/1‑140 (MQ=255)

                                               cggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGt                                                                                   >  2:528106/1‑140 (MQ=255)

                                               cggcgATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGt                                                                                   >  2:169798/1‑140 (MQ=255)

                                                                  ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGGAGACGTGATGCTCGACCGCCCGGGATACGGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  2:843060/140‑1 (MQ=255)

                                                                  ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                >  1:674754/1‑140 (MQ=255)

                                                                  ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  2:507700/140‑1 (MQ=255)

                                                                  ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  1:480586/140‑1 (MQ=255)

                                                                  ggTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATcgcg                                                                <  2:674754/140‑1 (MQ=255)

                                                                      ccAGCGAAAGCCTGTTCTGGTTTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTgcg                                                            <  2:792772/140‑1 (MQ=255)

                                                                      ccAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTgcg                                                            <  1:169798/140‑1 (MQ=255)

                                                                                   gTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCg                                               <  2:721670/140‑1 (MQ=255)

                                                                                     tCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGa                                             <  1:528106/140‑1 (MQ=255)

                                                                                              cGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGAt                                    >  1:500280/1‑140 (MQ=255)

                                                                                                               cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   >  2:132429/1‑140 (MQ=255)

                                                                                                               cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   >  2:350272/1‑140 (MQ=255)

                                                                                                               cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   >  1:705925/1‑140 (MQ=255)

                                                                                                               cGTGATGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTc                   <  1:256921/140‑1 (MQ=255)

                                                                                                                   aTGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCt               <  2:126115/140‑1 (MQ=255)

                                                                                                                   aTGCTCGACCGCCCGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCt               <  2:500280/140‑1 (MQ=255)

                                                                                                                                cGGGATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTg  >  2:122909/1‑140 (MQ=255)

TGGCCCAGCGCTGACCTTCGCCCTGCGTGAAGCTCTGTTCTCTCTGGCGGCGATTGAAGAAGAACTGGTGCCAGCGAAAGCCTGTTCTGGTCTGCGTCAGGTGCTGGAAGACGTGATGCTCGACCGCCCGGAATACTGGCAAAGCCACTACCACGGTGACGGCAACGCGCGTCGTCTGGCGCGTGGTTATAGCTACTCGGATCGCGTGCGCTATTACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTG  >  NC_000913/3279768‑3280035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A