Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	123	51.0	1731148	99.3%	1719029	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,109	T→C	L127L (TTA→TTG)	nanR ←	DNA‑binding transcriptional dual regulator NanR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,109	0	T	C	100.0%	161.0 / NA	45	L127L (TTA→TTG)	nanR	DNA‑binding transcriptional dual regulator NanR
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/26); total (19/26)

CGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCACC  >  NC_000913/3373973‑3374238

cGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAAt                                                                                                                                >  2:471362/1‑140 (MQ=255)

      aaCGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCg                                                                                                                          >  1:697568/1‑140 (MQ=255)

        cGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGaa                                                                                                                        <  1:522276/140‑1 (MQ=255)

          aatgaatgCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAAt                                                                                                                      <  1:309120/140‑1 (MQ=255)

                tgCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCaa                                                                                                                <  2:397009/140‑1 (MQ=255)

                tgCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCaa                                                                                                                >  1:682944/1‑140 (MQ=255)

                     cgTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCa                                                                                                           >  2:524498/1‑140 (MQ=255)

                      gttgttATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCAc                                                                                                          <  2:131390/140‑1 (MQ=255)

                              ccAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATga                                                                                                  <  2:596534/140‑1 (MQ=255)

                                  cGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATga                                                                                                  <  2:506618/136‑1 (MQ=255)

                                  cGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATga                                                                                                  >  1:506618/1‑136 (MQ=255)

                                  cGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGaaa                                                                                              >  1:133071/1‑140 (MQ=255)

                                        actgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAAt                                                                                        >  1:673128/1‑140 (MQ=255)

                                        actgTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAAt                                                                                        >  2:524761/1‑140 (MQ=255)

                                              gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCg                                                                                  <  2:686114/140‑1 (MQ=255)

                                              gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCg                                                                                  <  1:524761/140‑1 (MQ=255)

                                              gATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCg                                                                                  <  2:133071/140‑1 (MQ=255)

                                                      gTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCg                                                                          >  1:6034/1‑140 (MQ=255)

                                                        gCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        <  1:471362/140‑1 (MQ=255)

                                                            ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        >  1:466219/1‑136 (MQ=255)

                                                            ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        <  2:466219/136‑1 (MQ=255)

                                                            ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGc                                                                    >  2:836341/1‑140 (MQ=255)

                                                            ttGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGc                                                                    <  1:843354/140‑1 (MQ=255)

                                                              gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        >  1:571445/1‑134 (MQ=255)

                                                              gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGa                                                                        <  2:571445/134‑1 (MQ=255)

                                                              gCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTc                                                                  <  2:146250/140‑1 (MQ=255)

                                                                cAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCAc                                                                <  2:690127/140‑1 (MQ=255)

                                                                cAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCAc                                                                <  2:682944/140‑1 (MQ=255)

                                                                   cAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCga                                                             >  2:493121/1‑140 (MQ=255)

                                                                          aTTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCCCCAGACAGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAgt                                                      <  2:802703/140‑1 (MQ=255)

                                                                          aTTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAgt                                                      <  2:391297/140‑1 (MQ=255)

                                                                               cTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCg                                                 <  1:854272/140‑1 (MQ=255)

                                                                                   tCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGa                                             <  1:379961/140‑1 (MQ=255)

                                                                                     ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAg                                           <  1:836341/140‑1 (MQ=255)

                                                                                     ggTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAg                                           <  2:697568/140‑1 (MQ=255)

                                                                                                        tAGCGCACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGcgcg                        >  2:32407/1‑140 (MQ=255)

                                                                                                           cgcACCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTc                     >  2:710985/1‑140 (MQ=255)

                                                                                                              aCCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGcc                  >  1:708088/1‑140 (MQ=255)

                                                                                                              aCCAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGcc                  <  2:695553/140‑1 (MQ=255)

                                                                                                               ccAGACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCg                 >  2:841328/1‑140 (MQ=255)

                                                                                                                  gACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCgttg              <  2:6034/140‑1 (MQ=255)

                                                                                                                  gACTGGATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCgttg              >  1:634919/1‑140 (MQ=255)

                                                                                                                       gATTCAAAGAACAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTAt         <  1:841328/140‑1 (MQ=255)

                                                                                                                                  cAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCAcc  >  2:307922/1‑136 (MQ=255)

                                                                                                                                  cAGACGCAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCAcc  <  1:307922/136‑1 (MQ=255)

CGTCTGAACGAATGAATGCCGCGTTGTTATCCAGCGACTGACTGTTGATTTCCAGTGCTTTTGCCAGCAAATCGATTTGCTCATCGGTGGCATGTTCAGCCGCATAGCGCACCAGACTGGATTCAAAGAACAGACGTAATTGTTCGAAATGGGCAATCCCACCGGGATGAGAAAGGAAATCTTTCGCCATGCCGGAAAGCTCACCGATGATAGTGTCCGCAGAAGGACGCGAGACGCGAGCGCGTTCGCCGTTGTTTATTTGCACC  >  NC_000913/3373973‑3374238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A