Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F60 I1 R1	17	145.5	4959514	98.5%	4885121	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	100.0%	203.9 / NA	65	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (35/30); total (35/30)

ATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAT‑GGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196086‑1196312

atcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑ggg                                                                                         <  1:893623/140‑1 (MQ=255)

   gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTg                                                                                      >  2:140549/1‑140 (MQ=255)

   gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTg                                                                                      >  2:2188077/1‑140 (MQ=255)

    ccccTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCTGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTgg                                                                                     <  1:1612803/140‑1 (MQ=255)

        tGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGActg                                                                                 >  2:1618013/1‑138 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGActga                                                                                <  2:1011276/140‑4 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  1:1011276/1‑140 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  1:1525592/1‑140 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  2:572323/1‑140 (MQ=255)

           tGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAg                                                                              <  1:572323/140‑1 (MQ=255)

               aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGc                                                                          >  2:2020113/1‑140 (MQ=255)

               aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGc                                                                          >  1:1370914/1‑140 (MQ=255)

               aaCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGc                                                                          <  2:1825161/140‑1 (MQ=255)

                aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCt                                                                         <  1:1618013/140‑1 (MQ=255)

                  aTCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGa                                                                       >  2:223781/1‑140 (MQ=255)

                   tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  1:1063905/140‑1 (MQ=255)

                   tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  2:1607978/140‑1 (MQ=255)

                   tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  2:1525592/140‑1 (MQ=255)

                   tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  1:2020113/140‑1 (MQ=255)

                    cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  1:1400644/1‑139 (MQ=255)

                    cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:1899858/1‑139 (MQ=255)

                    cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:1786139/1‑139 (MQ=255)

                    cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:775379/1‑139 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGCAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCCCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCgg                                                                           >  2:881020/1‑133 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCgg                                                                           <  1:881020/133‑1 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  1:186331/1‑138 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  2:1266501/1‑138 (MQ=255)

                      gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  1:1696246/140‑4 (MQ=255)

                      gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  1:223781/140‑4 (MQ=255)

                       tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctga                                                                  <  2:1370914/140‑5 (MQ=255)

                         aCGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGAtt                                                                >  1:2373483/1‑140 (MQ=255)

                            aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   >  2:276705/1‑131 (MQ=255)

                            aaTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  1:276705/134‑4 (MQ=255)

                             aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:775379/140‑1 (MQ=38)

                             aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:1102737/140‑1 (MQ=38)

                             aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:1786139/140‑1 (MQ=255)

                                 gcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg                                                        >  2:2466304/1‑140 (MQ=255)

                                  cccTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGt                                                       <  1:1922435/140‑1 (MQ=37)

                                      gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                   >  2:1049709/1‑140 (MQ=255)

                                      gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                   >  2:2011025/1‑140 (MQ=255)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        <  1:79566/140‑1 (MQ=25)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  2:520115/1‑140 (MQ=255)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  1:1036089/1‑140 (MQ=255)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  2:274033/1‑140 (MQ=255)

                                                   cacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCa                                       <  2:1368856/140‑1 (MQ=25)

                                                       gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc                                  <  1:1558048/140‑2 (MQ=21)

                                                       gTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc                                  <  1:2466304/140‑2 (MQ=21)

                                                          cTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaag                               >  1:1688271/1‑139 (MQ=21)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                             <  2:1713433/124‑1 (MQ=25)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                             >  1:1713433/1‑124 (MQ=255)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatgcc                                  <  2:513605/135‑5 (MQ=21)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc                                  >  1:513605/1‑134 (MQ=21)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  1:140417/1‑140 (MQ=18)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  2:149188/1‑140 (MQ=18)

                                                             cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc                            >  1:2335776/1‑140 (MQ=18)

                                                             cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc                            >  2:857784/1‑140 (MQ=18)

                                                              gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                           <  2:2335776/140‑1 (MQ=18)

                                                                cGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTa                         >  1:1062565/1‑140 (MQ=18)

                                                                  aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc                       >  1:1703515/1‑140 (MQ=18)

                                                                        gCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGa                 <  2:1400644/140‑1 (MQ=17)

                                                                            gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc             >  1:1495979/1‑140 (MQ=17)

                                                                            gTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc             >  2:1730819/1‑140 (MQ=17)

                                                                              cAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa           <  2:1489237/140‑1 (MQ=14)

                                                                              cAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa           <  1:1049709/140‑1 (MQ=14)

                                                                                ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac         <  1:857784/140‑3 (MQ=14)

                                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat  >  2:2155257/1‑140 (MQ=11)

ATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAT‑GGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196086‑1196312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A