Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A31 F60 I1 R1	19	77.8	2638074	98.7%	2603779	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,196,220	C→T	H366H (CAC→CAT)	icd →	isocitrate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,196,220	0	C	T	100.0%	126.8 / NA	44	H366H (CAC→CAT)	icd	isocitrate dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/22); total (22/22)

ATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAT‑GGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196086‑1196312

atcgCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑ggg                                                                                         <  1:20274/140‑1 (MQ=255)

   gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTg                                                                                      >  1:177284/1‑140 (MQ=255)

        tGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGActg                                                                                 >  1:1002558/1‑138 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  2:405908/1‑140 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  1:365260/1‑140 (MQ=255)

         ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGa                                                                                >  2:762190/1‑140 (MQ=255)

                aCATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCt                                                                         <  1:314731/140‑1 (MQ=255)

                   tCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAc                                                                      <  1:762190/140‑1 (MQ=255)

                    cGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcc                                                                     >  2:721780/1‑139 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  2:464596/1‑138 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  1:910448/1‑138 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    <  1:563015/140‑3 (MQ=255)

                     ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcct                                                                    >  2:387352/1‑138 (MQ=255)

                      gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctg                                                                   <  2:365260/140‑4 (MQ=255)

                       tGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGAcctga                                                                  <  2:111292/140‑5 (MQ=255)

                             aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:387352/140‑1 (MQ=38)

                             aTGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTa                                                            <  1:721780/140‑1 (MQ=255)

                                cgcCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAg                                                         >  1:455111/1‑140 (MQ=255)

                                      gTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATgg                                                   <  1:874332/140‑1 (MQ=37)

                                       tttGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTATTAAAGGTATGGa                                                  <  1:507990/140‑1 (MQ=37)

                                          gAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAgg                                               <  2:281147/140‑1 (MQ=37)

                                            aGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcg                                             <  1:405908/140‑1 (MQ=25)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        <  1:1037809/140‑1 (MQ=25)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        <  1:685446/140‑1 (MQ=25)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  1:439060/1‑140 (MQ=255)

                                                 cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc                                        >  1:425907/1‑140 (MQ=255)

                                                    acGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaat                                     <  1:501157/140‑2 (MQ=25)

                                                           tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAGGgcg                                             >  1:641663/1‑125 (MQ=25)

                                                           tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAGGgcg                                             <  2:641663/125‑1 (MQ=25)

                                                           tGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaaga                              >  2:200049/1‑138 (MQ=21)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                <  1:535532/137‑1 (MQ=21)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa                                >  2:535532/1‑137 (MQ=21)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  1:873657/1‑140 (MQ=18)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  1:6523/1‑140 (MQ=18)

                                                            gcgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAc                             >  1:324497/1‑140 (MQ=18)

                                                             cgcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc                            >  2:782770/1‑140 (MQ=18)

                                                              gcCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCg                           <  2:425907/140‑1 (MQ=18)

                                                                             tCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg            >  1:462905/1‑140 (MQ=17)

                                                                               aGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATATGGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGa           <  2:123181/140‑1 (MQ=14)

                                                                                ggACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACATATGACTTCGaac         <  2:439060/140‑3 (MQ=14)

                                                                                  aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt       <  2:455111/140‑1 (MQ=11)

                                                                                  aCAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt       <  1:40309/140‑1 (MQ=11)

                                                                                      aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga   >  2:464599/1‑140 (MQ=11)

                                                                                      aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga   >  1:1183681/1‑140 (MQ=11)

                                                                                       gTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCATAT‑GGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgat  >  2:568309/1‑140 (MQ=11)

ATCGCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACAT‑GGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGAT  >  NC_000913/1196086‑1196312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A