Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A19 F60 I1 R1	24	40.0	1367016	98.7%	1349244	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,031,483	A→T	intergenic (‑65/+142)	yccA ← / ← serT	modulator of FtsH protease/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,031,483	0	A	T	100.0%	175.3 / NA	50	intergenic (‑65/+142)	yccA/serT	modulator of FtsH protease/tRNA‑Ser
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (28/22); total (28/22)

GTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACC  >  NC_000913/1031348‑1031612

gTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAg                                                                                                                               <  1:672135/140‑1 (MQ=255)

     aCGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTa                                                                                                                          >  1:146988/1‑140 (MQ=255)

           caCCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATc                                                                                                                    >  2:342871/1‑140 (MQ=255)

               ttATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTa                                                                                                                <  1:238051/140‑1 (MQ=255)

                tATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTAc                                                                                                               <  1:433335/140‑1 (MQ=255)

                    ggTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCAt                                                                                                           <  2:206070/140‑1 (MQ=255)

                        cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTAcc                                                                                                                        <  2:116681/123‑1 (MQ=255)

                        cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTAcc                                                                                                                        >  1:116681/1‑123 (MQ=255)

                        cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGa                                                                                                       >  1:3438/1‑140 (MQ=255)

                        cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGa                                                                                                       >  2:460655/1‑140 (MQ=255)

                        cTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGa                                                                                                       >  1:569456/1‑140 (MQ=255)

                                tGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAAc                                                                                               <  2:569456/140‑1 (MQ=255)

                                tGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAAc                                                                                               >  2:331005/1‑140 (MQ=255)

                                   tGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                                                                                 >  2:632358/1‑135 (MQ=255)

                                   tGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                                                                                 <  1:632358/135‑1 (MQ=255)

                                   tGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                                                                                 <  1:182783/135‑1 (MQ=255)

                                   tGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                                                                                 >  2:182783/1‑135 (MQ=255)

                                          tCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACa                                                                                     >  2:670543/1‑140 (MQ=255)

                                             tgtgAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                                                                                 >  2:538953/1‑125 (MQ=255)

                                             tgtgAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGa                                                                                                 <  1:538953/125‑1 (MQ=255)

                                                         aCAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTc                                                                      >  1:628479/1‑140 (MQ=255)

                                                                 aTCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCa                                                              >  2:284982/1‑140 (MQ=255)

                                                                  tCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCaa                                                             <  2:3438/140‑1 (MQ=255)

                                                                     aTTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAata                                                          >  2:85410/1‑140 (MQ=255)

                                                                           tCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGc                                                    >  1:676371/1‑140 (MQ=255)

                                                                              ctctctATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCt                                                 >  2:602911/1‑140 (MQ=255)

                                                                                         aGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGa                                      <  1:85410/140‑1 (MQ=255)

                                                                                         aGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGa                                      <  2:628479/140‑1 (MQ=255)

                                                                                           aTGTAATTAATTAAGCAGCATAATGGTAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACa                                    >  2:166737/1‑140 (MQ=255)

                                                                                           aTGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACa                                    >  2:84489/1‑140 (MQ=255)

                                                                                               aattaattAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCa                                <  1:460655/140‑1 (MQ=255)

                                                                                               aattaattAAGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCa                                <  1:602911/140‑1 (MQ=255)

                                                                                                 ttaattaaGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAGTTTCGACAGGCaaa                              <  2:645306/140‑1 (MQ=255)

                                                                                                     ttaaGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGaa                          >  1:297233/1‑140 (MQ=255)

                                                                                                      taaGCAGCATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGaaa                         <  1:166737/140‑1 (MQ=255)

                                                                                                        agcagcATAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGaaaaa                       <  1:670573/140‑1 (MQ=255)

                                                                                                             caTAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCaaa                              >  2:354441/1‑128 (MQ=255)

                                                                                                             caTAATGATAATGCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCaaa                              <  1:354441/128‑1 (MQ=255)

                                                                                                               taatgataatgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATAT‑‑CGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGc              >  2:169409/1‑140 (MQ=255)

                                                                                                                       atgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGt                  >  1:205551/1‑130 (MQ=255)

                                                                                                                       atgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGt                  <  2:205551/130‑1 (MQ=255)

                                                                                                                       atgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAg        >  1:353594/1‑140 (MQ=255)

                                                                                                                       atgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAg        >  1:194452/1‑140 (MQ=255)

                                                                                                                       atgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAg        >  2:302277/1‑140 (MQ=255)

                                                                                                                       atgCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAg        >  2:272760/1‑140 (MQ=255)

                                                                                                                         gCGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCt      <  2:297233/140‑1 (MQ=255)

                                                                                                                          cGTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTa     >  1:227565/1‑140 (MQ=255)

                                                                                                                           gTAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTaa    <  1:272760/140‑1 (MQ=255)

                                                                                                                            tAAGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAAc   >  2:172007/1‑140 (MQ=255)

                                                                                                                             aaGGGCACCCTGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAAcc  <  1:448466/140‑1 (MQ=255)

GTATTACGCAGCACCTTATGGGTGCTAAGCAGTGATGTACGGTCATGTGAAGAACTAACAATACGATCCATTGAGTCACTCTCTATGACAGATGTAATTAATTAAGCAGCATAATGATAATGCGTAAGGGCACCCAGAAGTTTTACCCATCTTTACGCATTTGATCTGGAACAGGTTTAACAGCGGATTATCAGGTCATTAAGCAAATATAACGCCCTGAGAATTTCGACAGGCAAAAGAAAAAGGGGTTAGCATTTAGCTAACC  >  NC_000913/1031348‑1031612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A