Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F60 I1 R1	25	51.1	1725140	98.8%	1704438	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,048,915	T→G	T140P (ACC→CCC)	gfcB ←	lipoprotein GfcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,048,915	0	T	G	100.0%	217.8 / NA	57	T140P (ACC→CCC)	gfcB	lipoprotein GfcB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (32/25); total (32/25)

GACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGTTTGACG  >  NC_000913/1048781‑1049051

gACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCag                                                                                                                                     <  1:24462/140‑1 (MQ=255)

gACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCag                                                                                                                                     <  1:450488/140‑1 (MQ=255)

           tgtTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATgg                                                                                                                          >  2:105338/1‑140 (MQ=255)

                 gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCt                                                                                                                    >  1:759515/1‑140 (MQ=255)

                 gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCt                                                                                                                    >  2:67163/1‑140 (MQ=255)

                    aCTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCa                                                                                                                 <  2:188937/140‑1 (MQ=255)

                      tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATg                                                                                                                           <  2:468522/128‑1 (MQ=255)

                      tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATg                                                                                                                           >  1:468522/1‑128 (MQ=255)

                      tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                               <  2:759515/140‑1 (MQ=255)

                          tttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCag                                                                                                           <  1:67163/140‑1 (MQ=255)

                          tttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCag                                                                                                           >  2:196731/1‑140 (MQ=255)

                                  cgcgTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATc                                                                                                   >  1:480280/1‑140 (MQ=255)

                                          aGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTcc                                                                                           >  1:290084/1‑140 (MQ=255)

                                                     ccTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCgg                                                                                      <  2:768427/134‑1 (MQ=255)

                                                     ccTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCgg                                                                                      >  1:768427/1‑134 (MQ=255)

                                                         ccAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                               >  1:376899/1‑137 (MQ=255)

                                                         ccAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                               <  2:376899/137‑1 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCTGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  1:22147/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             <  2:178333/140‑1 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  2:105355/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  2:18460/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             <  1:756038/140‑1 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  2:292530/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  1:635841/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  1:484929/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  2:797285/1‑140 (MQ=255)

                                                                        cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                             >  1:390372/1‑140 (MQ=255)

                                                                           aTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAATGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTAc                                                          >  1:521849/1‑140 (MQ=255)

                                                                           aTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTAc                                                          <  2:405992/140‑1 (MQ=255)

                                                                           aTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTAc                                                          >  1:405992/1‑140 (MQ=255)

                                                                              gCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCa                                                       <  1:590033/140‑1 (MQ=255)

                                                                                     gCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCTGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                                <  2:22147/140‑1 (MQ=255)

                                                                                          atTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGcc                                           <  2:480294/140‑1 (MQ=255)

                                                                                            tCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCg                                         <  1:841529/140‑1 (MQ=255)

                                                                                              aGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGa                                       >  2:188892/1‑140 (MQ=255)

                                                                                                cGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAAt                                     >  2:143059/1‑140 (MQ=255)

                                                                                                     ctAATCCAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTc                                <  1:26674/140‑1 (MQ=255)

                                                                                                          ccAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTc                           >  2:405382/1‑140 (MQ=255)

                                                                                                          ccAGCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTc                           <  2:480280/140‑1 (MQ=255)

                                                                                                             gCAAAGGAAAAAGTAGAAGTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag                        >  1:89361/1‑140 (MQ=255)

                                                                                                             gCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag                        >  2:510108/1‑140 (MQ=255)

                                                                                                             gCAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag                        >  1:189111/1‑140 (MQ=255)

                                                                                                              cAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTGACTTCca                         <  2:455324/138‑1 (MQ=255)

                                                                                                              cAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTGACTTCca                         >  1:455324/1‑138 (MQ=255)

                                                                                                              cAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagc                       >  1:304587/1‑140 (MQ=255)

                                                                                                              cAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCca                         <  2:1881/138‑1 (MQ=255)

                                                                                                              cAAAGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCca                         >  1:1881/1‑138 (MQ=255)

                                                                                                               aaaGGAAAAAGTAGAAGTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca                      <  2:89361/140‑1 (MQ=255)

                                                                                                               aaaGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca                      <  1:292530/140‑1 (MQ=255)

                                                                                                               aaaGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca                      <  1:18460/140‑1 (MQ=255)

                                                                                                                 aGGAAAAAGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGa                    <  1:510108/140‑1 (MQ=255)

                                                                                                                        aGTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGattattat             >  2:41162/1‑140 (MQ=255)

                                                                                                                         gTAGAACTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGattattatt            <  1:797285/140‑1 (MQ=255)

                                                                                                                             aaCTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGt        >  1:814861/1‑140 (MQ=255)

                                                                                                                             aaCTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGt        >  1:704857/1‑140 (MQ=255)

                                                                                                                              aCTCAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGtt       >  1:423329/1‑140 (MQ=255)

                                                                                                                                 cAGGGGCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGTTTGa    <  1:41162/140‑1 (MQ=255)

                                                                                                                                   ggggCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGTTTGACg  <  2:814861/140‑1 (MQ=255)

GACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATTAAGTTTGACG  >  NC_000913/1048781‑1049051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A