Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F60 I1 R1
|
17 |
33.2 |
1123130 |
98.8% |
1109652 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,116,548 |
A→C |
L12R (CTT→CGT) |
lpxL ← |
lauroyl acyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,116,548 | 0 | A | C | 100.0%
| 62.6
/ NA
| 18 | L12R (CTT→CGT) | lpxL | lauroyl acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (12/6); total (12/6) |
CGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAATTTGTGGCTGAAGACTGGGCGAAATTGCCG > NC_000913/1116417‑1116681
|
cgcgTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCg < 2:344495/140‑1 (MQ=255)
cATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACt < 2:428759/140‑1 (MQ=255)
aaCGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTg > 1:295912/1‑123 (MQ=255)
aaCGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTg < 2:295912/123‑1 (MQ=255)
aaCGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGt > 2:397501/1‑140 (MQ=255)
tCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGt > 1:519973/1‑140 (MQ=255)
gCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTAt > 1:14508/1‑140 (MQ=255)
ggTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATtgtat > 2:362810/1‑140 (MQ=255)
ggTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCATCATATCAATCCTGTTTTTCAACCTATTCGGGCAATtgtat > 1:52840/1‑140 (MQ=255)
gTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCggg < 1:540293/130‑1 (MQ=255)
gTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCggg > 2:540293/1‑130 (MQ=255)
aGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATtgtatgta > 1:312789/1‑140 (MQ=255)
cAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGt > 1:479785/1‑140 (MQ=255)
cAATACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAAt > 2:351954/1‑140 (MQ=255)
tACCCAACCAGGTTAACCAATAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAATTtg < 2:519973/140‑1 (MQ=255)
aTAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAATTTGTGGCTGAAGACTGGGCGaa > 1:405171/1‑140 (MQ=255)
tAACGCGGATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAATTTGTGGCTGAAGACTGGGCGaaa > 2:102748/1‑140 (MQ=255)
ggATGACGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAATTTGTGGCTGAAGACTGGGCGAAATTGCcg < 2:312789/140‑1 (MQ=255)
|
CGCGTCGTTTCATAAAACGTAACGCCAGTTTTCCTAATCCACAACCGAGGCGGTAGATAACCGGGTAGGGCAATTGCACGACTAACCAAAGTACGCCAATACCCAACCAGGTTAACCAATAACGCGGATGAAGCAGTGCGGTGGAGAACTTGGGTAGATTCGTCATATCAATCCTGTTTTTCAACCTATTCGGGCAATTGTATGTATTGTCGCATTTTTTCGCCCGCAACCAAAATTTGTGGCTGAAGACTGGGCGAAATTGCCG > NC_000913/1116417‑1116681
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
