Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A19 F60 I1 R1 24 40.0 1367016 98.7% 1349244 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,577,441 A→G V173A (GTC→GCC)  mcrC ← 5‑methylcytosine‑specific restriction enzyme subunit McrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,577,4410AG100.0% 215.0 / NA 56V173A (GTC→GCC) mcrC5‑methylcytosine‑specific restriction enzyme subunit McrC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/29);  total (27/29)

TGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTT  >  NC_000913/4577305‑4577573
                                                                                                                                        |                                                                                                                                    
tgtgTTTGCAGATGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAACGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGa                                                                                                                                   >  2:645471/1‑140 (MQ=255)
  tgtTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATg                                                                                                                                 >  2:385462/1‑140 (MQ=255)
    tttGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGaa                                                                                                                               >  1:656797/1‑140 (MQ=255)
        cAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTg                                                                                                                           >  1:110205/1‑140 (MQ=255)
        cAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTg                                                                                                                           <  1:348713/140‑1 (MQ=255)
            cGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGa                                                                                                                       <  1:385462/140‑1 (MQ=255)
              ttAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGaca                                                                                                                     <  1:390892/140‑1 (MQ=255)
                     acgacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATaa                                                                                                              <  1:75848/140‑1 (MQ=255)
                       gacgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACg                                                                                                            >  1:378131/1‑140 (MQ=255)
                        acgGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGa                                                                                                           <  1:670256/140‑1 (MQ=255)
                            cAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAAttt                                                                                                       >  2:104639/1‑140 (MQ=255)
                                  tCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTataata                                                                                                 <  2:54820/140‑1 (MQ=255)
                                                ttGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTc                                                                                   <  1:104639/140‑1 (MQ=255)
                                                       aGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCAtt                                                                            >  2:435320/1‑140 (MQ=255)
                                                         tAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCa                                                                          <  2:671906/140‑1 (MQ=255)
                                                         tAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCa                                                                          <  2:656797/140‑1 (MQ=255)
                                                         tAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCa                                                                          <  1:435320/140‑1 (MQ=255)
                                                            tGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGAt                                                                       >  2:407553/1‑140 (MQ=255)
                                                             gACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATa                                                                      >  1:344386/1‑140 (MQ=255)
                                                                  ctctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGa                                                                 >  1:193959/1‑140 (MQ=255)
                                                                   tctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGAt                                                                       <  1:597525/133‑1 (MQ=255)
                                                                   tctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGAt                                                                       >  2:597525/1‑133 (MQ=255)
                                                                   tctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGaa                                                                >  1:604419/1‑140 (MQ=255)
                                                                   tctTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGaa                                                                >  1:650047/1‑140 (MQ=255)
                                                                        ttCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgct                                                           <  1:617548/140‑1 (MQ=255)
                                                                          cGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAAATTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAAtgctgc                                                         >  2:284322/1‑140 (MQ=255)
                                                                           ttttcattcAAAATCATAGAAACGGTAGTGTCGTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCg                                                        <  2:604419/139‑1 (MQ=255)
                                                                           gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATATCTGAAATGCTGCg                                                        <  2:344386/140‑1 (MQ=255)
                                                                           gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCg                                                        <  1:645471/140‑1 (MQ=255)
                                                                           gTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCg                                                        <  2:232339/140‑1 (MQ=255)
                                                                             ttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGa                                                      >  2:588718/1‑140 (MQ=255)
                                                                             ttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGa                                                      >  2:334400/1‑140 (MQ=255)
                                                                             ttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGa                                                      <  2:600652/140‑1 (MQ=255)
                                                                             ttctttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGa                                                      >  2:153985/1‑140 (MQ=255)
                                                                                 ttcAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTa                                                  >  1:172288/1‑140 (MQ=255)
                                                                                     aaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATg                                              <  2:172288/140‑1 (MQ=255)
                                                                                     aaaTCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATg                                              <  1:280619/140‑1 (MQ=255)
                                                                                        tCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAag                                           <  1:334400/140‑1 (MQ=255)
                                                                                         cATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAaga                                          <  1:484511/140‑1 (MQ=255)
                                                                                             gAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGc                                      <  1:23523/140‑1 (MQ=255)
                                                                                                aCGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTaa                                   >  2:176605/1‑140 (MQ=255)
                                                                                                    tAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGcc                               <  1:176605/140‑1 (MQ=255)
                                                                                                    tAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGcc                               >  1:328121/1‑140 (MQ=255)
                                                                                                    tAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGcc                               <  2:110205/140‑1 (MQ=255)
                                                                                                        gtCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGt                           >  1:53595/1‑140 (MQ=255)
                                                                                                         tCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTa                          >  1:206894/1‑140 (MQ=255)
                                                                                                         tCCTTTGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTa                          >  1:609838/1‑140 (MQ=255)
                                                                                                            tttGTTTTGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAAtt                       >  2:14490/1‑140 (MQ=255)
                                                                                                                 tttGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCta                  <  2:650047/140‑1 (MQ=255)
                                                                                                                   tGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCtata                >  2:88538/1‑140 (MQ=255)
                                                                                                                   tGACCTGGAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCtata                >  2:153969/1‑140 (MQ=255)
                                                                                                                          gAATAGAATTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGaa         <  2:68525/140‑1 (MQ=255)
                                                                                                                                aaTTATTGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCt   >  2:593268/1‑140 (MQ=255)
                                                                                                                                 attattGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt  <  2:193959/140‑1 (MQ=255)
                                                                                                                                 attattGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCtt  <  1:407553/140‑1 (MQ=255)
                                                                                                                                     ttGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAg     >  2:599030/1‑133 (MQ=255)
                                                                                                                                     ttGGCGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAg     <  1:599030/133‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TGTGTTTGCAGACGTTAATTCACGACGGCAAAATTCATAAAGAAACTTTTGATAAAGTAATGACATCTCTTTTTCGTTTCTTTCAAAATCATAGAAACGGTAGTGTCCTTTGTTTTGACCTGGAATAGAATTATTGACGATGAATTTGCAGACACTGATAACGAATTTATAATAACGCGTATTTTTTCCGCCATTCAGATAGCTGAAATGCTGCGGAGTTAAATGAAGAGTGCTAATGCCCGGTAATTTTCTATAAAGTGAACGAGCTT  >  NC_000913/4577305‑4577573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A