Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F60 I1 R1	18	43.3	1467046	98.8%	1449441	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	991,988	C→T	P456L (CCG→CTG)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	991,988	0	C	T	100.0%	129.0 / NA	37	P456L (CCG→CTG)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/16); total (21/16)

TGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGC  >  NC_000913/991854‑992119

tgatgGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGaaa                                                                                                                                <  1:217290/140‑1 (MQ=255)

        gTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAg                                                                                                                        >  2:387515/1‑140 (MQ=255)

         tGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGt                                                                                                                       <  2:555106/140‑1 (MQ=255)

         tGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGt                                                                                                                       >  1:145079/1‑140 (MQ=255)

         tGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGt                                                                                                                       >  1:485613/1‑140 (MQ=255)

          gCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTa                                                                                                                      >  2:66510/1‑140 (MQ=255)

          gCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTa                                                                                                                      <  1:550564/140‑1 (MQ=255)

                                tgCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAgcgc                                                                                                >  1:573851/1‑140 (MQ=255)

                                     gCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACgcc                                                                                           >  2:193464/1‑140 (MQ=255)

                                        aTGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACgccagc                                                                                        <  1:321173/140‑1 (MQ=255)

                                        aTGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACgccagc                                                                                        >  1:452836/1‑140 (MQ=255)

                                          ggAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACgccagcca                                                                                      <  2:439344/140‑1 (MQ=255)

                                               aTGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCg                                                                                 >  1:32045/1‑140 (MQ=255)

                                                                 tctcCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAg                                                               >  1:142577/1‑140 (MQ=255)

                                                                   tcCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGcc                                                             <  1:684559/140‑1 (MQ=255)

                                                                   tcCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGcc                                                             <  1:193464/140‑1 (MQ=255)

                                                                      cATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCt                                                          <  1:447058/140‑1 (MQ=255)

                                                                        tttCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGc                                                        >  2:182118/1‑140 (MQ=255)

                                                                             gccgTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATAtt                                                   >  1:478885/1‑140 (MQ=255)

                                                                             gccgTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATAtt                                                   >  1:313872/1‑140 (MQ=255)

                                                                                            aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    <  1:367817/140‑1 (MQ=255)

                                                                                            aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    <  2:603391/140‑1 (MQ=255)

                                                                                            aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    <  1:182118/140‑1 (MQ=255)

                                                                                            aGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    <  2:485613/140‑1 (MQ=255)

                                                                                                   tACACCGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATg                             >  2:264834/1‑140 (MQ=255)

                                                                                                        cGATTGTGACCGTCAAAGGCGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAAc                        >  2:214673/1‑140 (MQ=255)

                                                                                                        cGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    >  1:269067/1‑128 (MQ=255)

                                                                                                        cGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    <  2:242013/128‑1 (MQ=255)

                                                                                                        cGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    <  2:269067/128‑1 (MQ=255)

                                                                                                        cGATTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGaa                                    >  1:242013/1‑128 (MQ=255)

                                                                                                          aTTGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGa                      >  2:515459/1‑140 (MQ=255)

                                                                                                           ttGTGACCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGaa                     <  1:387515/140‑1 (MQ=255)

                                                                                                                aCCGTCAAAGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCaa                >  1:322640/1‑140 (MQ=255)

                                                                                                                       aaGACGACTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATc         <  2:313872/140‑1 (MQ=255)

                                                                                                                              cTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGc  >  1:115980/1‑140 (MQ=255)

                                                                                                                              cTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGc  >  1:98338/1‑140 (MQ=255)

                                                                                                                              cTACAATCTGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGc  >  1:353123/1‑140 (MQ=255)

TGATGGTAGTGCAGCGACCTGTGACGACTTTGTGCAGGCGATGGAAGATGCGTCGAATGTCGATCTCTCCCATTTCCGCCGTTGGTACAGCCAGTCCGGTACACCGATTGTGACCGTCAAAGACGACTACAATCCGGAAACCGAGCAGTACACCCTGACCATCAGCCAGCGCACGCCAGCCACGCCGGATCAGGCAGAAAAACAGCCGCTGCATATTCCGTTTGCCATCGAACTGTATGATAACGAAGGCAAAGTGATCCCGTTGC  >  NC_000913/991854‑992119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A