Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F60 I1 R1 25 51.1 1725140 98.8% 1704438 138.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,179,443 C→T L189L (CTG→TTG)  rplA → 50S ribosomal subunit protein L1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,179,4430CT100.0% 136.7 / NA 40L189L (CTG→TTG) rplA50S ribosomal subunit protein L1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/19);  total (21/19)

AACACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACCTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGT  >  NC_000913/4179313‑4179576
                                                                                                                                  |                                                                                                                                     
aaCACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAg                                                                                                                                 >  1:242899/1‑137 (MQ=255)
aaCACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAg                                                                                                                                 <  2:242899/137‑1 (MQ=255)
       aaCGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGt                                                                                                                       >  1:415982/1‑140 (MQ=255)
       aaCGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGt                                                                                                                       >  1:517526/1‑140 (MQ=255)
         cGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTg                                                                                                                     >  2:677848/1‑140 (MQ=255)
             gCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCt                                                                                                                 >  1:269904/1‑140 (MQ=255)
             gCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCt                                                                                                                 >  1:417239/1‑140 (MQ=255)
                gAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGaa                                                                                                              <  2:819335/140‑1 (MQ=255)
                      gTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGc                                                                                                        >  1:157364/1‑140 (MQ=255)
                      gTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGc                                                                                                        >  1:560379/1‑140 (MQ=255)
                      gTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGc                                                                                                        >  1:552437/1‑140 (MQ=255)
                             aCGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCg                                                                                                 >  1:696208/1‑140 (MQ=255)
                              cGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGa                                                                                                <  1:293223/140‑1 (MQ=255)
                                cTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACt                                                                                              <  2:560379/140‑1 (MQ=255)
                                 tAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTc                                                                                             >  1:271537/1‑140 (MQ=255)
                                        ggCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGaa                                                                                      <  2:417239/140‑1 (MQ=255)
                                         gCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAGAAAAGCAAAACCGACTCAGGCGaaa                                                                                     >  1:669575/1‑140 (MQ=255)
                                              gTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCgt                                                                                >  2:424177/1‑140 (MQ=255)
                                               ttCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCgtg                                                                               <  1:436566/140‑1 (MQ=255)
                                                 cGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTa                                                                             <  2:271537/140‑1 (MQ=255)
                                                   ttACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACa                                                                           >  2:183637/1‑140 (MQ=255)
                                                         tAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGa                                                                     >  1:555433/1‑140 (MQ=255)
                                                                 aaaaCGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGc                                                             <  2:415982/140‑1 (MQ=255)
                                                                       gCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTcc                                                       <  2:543704/140‑1 (MQ=255)
                                                                         atcatcCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTccac                                                     <  2:229756/140‑1 (MQ=255)
                                                                             tcCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTccaccacc                                                 <  2:269904/140‑1 (MQ=255)
                                                                                      ccaTCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCAt                                               <  1:810890/133‑1 (MQ=255)
                                                                                      ccaTCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCAt                                               >  2:810890/1‑133 (MQ=255)
                                                                                       caTCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAg                                       >  2:472166/1‑140 (MQ=255)
                                                                                        aTCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAgg                                      >  1:156199/1‑140 (MQ=255)
                                                                                               aaGTGGACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCa                               <  1:305984/140‑1 (MQ=255)
                                                                                                    gACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGa                          <  1:807197/140‑1 (MQ=255)
                                                                                                    gACTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGa                          >  2:807197/1‑140 (MQ=255)
                                                                                                     aCTTTGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGAc                         >  1:594972/1‑140 (MQ=255)
                                                                                                         tGACGCTGACAAACTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAgg                     <  2:157364/140‑1 (MQ=255)
                                                                                                                     aCTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAgcg         <  2:594972/140‑1 (MQ=255)
                                                                                                                      cTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAgcgc        <  2:156199/140‑1 (MQ=255)
                                                                                                                      cTGAAAGAAAACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAgcgc        <  1:472166/140‑1 (MQ=255)
                                                                                                                           agaaaACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTg   >  1:286747/1‑140 (MQ=255)
                                                                                                                            gaaaACTTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGt  <  2:663924/140‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                     
AACACCGAACGTTGCTGAAGCGGTTAAAAACGCTAAAGCTGGCCAGGTTCGTTACCGTAACGACAAAAACGGCATCATCCACACCACCATCGGTAAAGTGGACTTTGACGCTGACAAACTGAAAGAAAACCTGGAAGCTCTGCTGGTTGCGCTGAAAAAAGCAAAACCGACTCAGGCGAAAGGCGTGTACATCAAGAAAGTTAGCATCTCCACCACCATGGGTGCAGGTGTTGCAGTTGACCAGGCTGGCCTGAGCGCTTCTGT  >  NC_000913/4179313‑4179576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A