Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A22 F60 I1 R1	18	54.7	1860826	98.9%	1840356	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,192,818	T→C	D7G (GAT→GGT)	thiS ←	sulfur carrier protein ThiS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,192,818	0	T	C	100.0%	198.6 / NA	52	D7G (GAT→GGT)	thiS	sulfur carrier protein ThiS
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/30); total (22/30)

CTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGTCTCTATACCGCTTAA  >  NC_000913/4192682‑4192952

cTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGt                                                                                                                                     <  1:113475/140‑1 (MQ=255)

     cGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAAc                                                                                                                                <  2:844195/140‑1 (MQ=255)

     cGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAAc                                                                                                                                <  1:236585/140‑1 (MQ=255)

          tGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGAt                                                                                                                           >  1:49785/1‑140 (MQ=255)

           gTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATc                                                                                                                          <  2:530477/140‑1 (MQ=255)

             tGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGt                                                                                                                                     <  2:901678/127‑1 (MQ=255)

             tGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGt                                                                                                                                     >  1:901678/1‑127 (MQ=255)

                   cACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGc                                                                                                                  <  1:595656/140‑1 (MQ=255)

                      tGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTtc                                                                                                               <  1:165147/140‑1 (MQ=255)

                           aCGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCg                                                                                                          >  1:542242/1‑140 (MQ=255)

                                  aCGATTTGCTGATTAATCGCCGGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCtt                                                                                                                >  1:901458/1‑127 (MQ=255)

                                  aCGATTTGCTGATTAATCGCCGGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCtt                                                                                                                <  2:901458/127‑1 (MQ=255)

                                  aCGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCg                                                                                                   >  2:319965/1‑140 (MQ=255)

                                       ttGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCaa                                                                                              >  1:373438/1‑140 (MQ=255)

                                              ttAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTgg                                                                                       >  2:853525/1‑140 (MQ=255)

                                              ttAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTgg                                                                                       <  1:319965/140‑1 (MQ=255)

                                                     ccAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTggcgcggcg                                                                                <  1:452497/140‑1 (MQ=255)

                                                     ccAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTggcgcggcg                                                                                <  2:276696/140‑1 (MQ=255)

                                                     ccAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTggcgcggcg                                                                                <  2:683500/140‑1 (MQ=255)

                                                     ccAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTggcgcggcg                                                                                <  2:589269/140‑1 (MQ=255)

                                                                    cTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTgcg                                                                 >  1:367178/1‑140 (MQ=255)

                                                                             ggTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCt                                                        <  1:39906/140‑1 (MQ=255)

                                                                                  aGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTgg                                                          <  1:249973/133‑1 (MQ=255)

                                                                                  aGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTgg                                                          >  2:249973/1‑133 (MQ=255)

                                                                                       cTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCg                                              >  2:442067/1‑140 (MQ=255)

                                                                                          cAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCg                                           <  2:367178/140‑1 (MQ=255)

                                                                                             tagtTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAAc                                        >  1:542793/1‑140 (MQ=255)

                                                                                             tagtTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAAc                                        >  1:373395/1‑140 (MQ=255)

                                                                                                ttCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAGCCACTGGCGCGGCGCAACGGCAGGCTGCGCCACTGGCTCGGTTTACCGTCGAACAGt                                     <  2:464127/140‑1 (MQ=255)

                                                                                                ttCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGt                                     <  2:542242/140‑1 (MQ=255)

                                                                                                ttCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGt                                     >  1:512438/1‑140 (MQ=255)

                                                                                                    tGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGa                                 >  2:460990/1‑140 (MQ=255)

                                                                                                    tGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGa                                 >  1:295618/1‑140 (MQ=255)

                                                                                                       aCAGTTTGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGtt                              >  2:516833/1‑140 (MQ=255)

                                                                                                           tttGCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTcc                          >  2:790167/1‑140 (MQ=255)

                                                                                                              gCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGc                       <  2:373395/140‑1 (MQ=255)

                                                                                                              gCCCGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGc                       >  2:130012/1‑140 (MQ=255)

                                                                                                               cccGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCa                      <  2:373438/140‑1 (MQ=255)

                                                                                                               cccGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCa                      <  2:512438/140‑1 (MQ=255)

                                                                                                                ccGGCGGCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAg                     >  2:306890/1‑140 (MQ=255)

                                                                                                                 cggcggCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    <  2:542793/140‑1 (MQ=255)

                                                                                                                 cggcggCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    <  1:130012/140‑1 (MQ=255)

                                                                                                                 cggcggCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    <  2:49785/140‑1 (MQ=255)

                                                                                                                 cggcggCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    <  1:516833/140‑1 (MQ=255)

                                                                                                                 cggcggCGCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    <  1:442067/140‑1 (MQ=255)

                                                                                                                   gcggcgCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    >  2:584573/1‑138 (MQ=255)

                                                                                                                   gcggcgCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAgg                    <  1:584573/138‑1 (MQ=255)

                                                                                                                     ggcgCACTGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGtc                >  1:702940/1‑140 (MQ=255)

                                                                                                                            tGCATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCTGGCAGCCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGTCTCTATAc         <  2:131955/140‑1 (MQ=255)

                                                                                                                              cATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGtct               <  2:261232/132‑1 (MQ=255)

                                                                                                                              cATCGCTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGtct               >  1:261232/1‑132 (MQ=255)

                                                                                                                                   cTTGACCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGTCTCTATACCGCTtaa  <  1:853525/140‑1 (MQ=255)

CTGCACGATATGTTGCGCCCACTGCTCACGCGGGACGATTTGCTGATTAATCGCCAGAGCCGCGCCCGCTTGTCGTTGGTCGAGTTGCTCCAGTAGTTCGTGAACAGTTTGCCCGGCGGCGCACTGCATCGCTTGATCGTTAAACAGGATCTGCATTGCTTCCTCCGCATACCGGGCAACCACTGGCGCGGCGCAACGCCAGGCTGCGCCACTGGCTCGATTTACCGTCGAACAGTCGGAGTTCTCCCGCAGGTGTCTCTATACCGCTTAA  >  NC_000913/4192682‑4192952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A