Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F60 I1 R1	19	88.7	3019690	98.6%	2977414	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,195,643	C→T	A153T (GCC→ACC)	thiC ←	phosphomethylpyrimidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,195,643	0	C	T	98.4%	186.4 / NA	61	A153T (GCC→ACC)	thiC	phosphomethylpyrimidine synthase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (25/35); minor base A (1/0); total (26/35)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTTTCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTA  >  NC_000913/4195507‑4195770

atatTTTCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTa                                                                                                                              <  1:687147/140‑1 (MQ=255)

atatTTTCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTa                                                                                                                              >  1:497725/1‑140 (MQ=255)

atatTTTCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTa                                                                                                                              >  1:133816/1‑140 (MQ=255)

      tCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAg                                                                                                                        <  1:123158/140‑1 (MQ=255)

      tCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAg                                                                                                                        <  1:37819/140‑1 (MQ=255)

      tCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAg                                                                                                                        <  2:582152/140‑1 (MQ=255)

        cGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAg                                                                                                                        >  1:600450/1‑138 (MQ=255)

        cGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAg                                                                                                                        <  2:600450/138‑1 (MQ=255)

        cGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGtt                                                                                                                      <  1:1286073/140‑1 (MQ=255)

        cGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGtt                                                                                                                      >  1:283942/1‑140 (MQ=255)

              aTGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGa                                                                                                                >  2:822885/1‑140 (MQ=255)

                 tgCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGacac                                                                                                             <  1:1062804/140‑1 (MQ=255)

                        aaGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTcc                                                                                                      >  1:1336213/1‑140 (MQ=255)

                         aGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCt                                                                                                     >  2:275847/1‑140 (MQ=255)

                               ttCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTg                                                                                               <  1:36116/140‑1 (MQ=255)

                                tCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTgg                                                                                              >  2:177752/1‑140 (MQ=255)

                                     gATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGt                                                                                         <  1:231956/140‑1 (MQ=255)

                                     gATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGt                                                                                         >  2:95004/1‑140 (MQ=255)

                                     gATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGt                                                                                         >  1:1263651/1‑140 (MQ=255)

                                     gATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGt                                                                                         <  1:651670/140‑1 (MQ=255)

                                     gATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGCAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGt                                                                                         >  1:963900/1‑140 (MQ=255)

                                       tGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGttt                                                                                       <  2:133816/140‑1 (MQ=255)

                                        gCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGtttt                                                                                      <  1:275847/140‑1 (MQ=255)

                                         cTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGcgcg                                                                                          >  2:949458/1‑135 (MQ=255)

                                         cTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGcgcg                                                                                          <  1:949458/135‑1 (MQ=255)

                                          tggtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTTGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTgg                                                                                    >  2:701223/1‑140 (MQ=255)

                                            gtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGtttt                                                                                      >  2:946116/1‑136 (MQ=255)

                                            gtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGtttt                                                                                      <  1:946116/136‑1 (MQ=255)

                                            gtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTg                                                                                     <  1:1483568/137‑1 (MQ=255)

                                            gtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTg                                                                                     >  2:1483568/1‑137 (MQ=255)

                                            gtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGgtg                                                                                  >  1:1256649/1‑140 (MQ=255)

                                            gtggCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGGGCAGTTGGGGGACACGGCGTCCTGCTTTTGCGCGGTTTgggg                                                                                  >  2:231956/1‑138 (MQ=255)

                                                  tAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTa                                                                            <  2:1256649/140‑1 (MQ=255)

                                                       ccTCGCTACGGATGCGCTCGCGGCCCATATTCTTGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTAcgccg                                                                       <  1:701223/140‑1 (MQ=255)

                                                          cGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTa                                                                    >  2:1405174/1‑140 (MQ=255)

                                                             tACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTaaaa                                                                 <  2:532689/140‑1 (MQ=255)

                                                                   tGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGt                                                           <  1:95004/140‑1 (MQ=255)

                                                                     cgcTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTtc                                                         >  1:1231519/1‑140 (MQ=255)

                                                                       cTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTAcgc                                                                         >  1:1040610/1‑122 (MQ=255)

                                                                       cTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTAcgc                                                                         <  2:1040610/122‑1 (MQ=255)

                                                                       cTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTtcgt                                                       >  1:697762/1‑140 (MQ=255)

                                                                       cTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTtcgt                                                       >  1:156788/1‑140 (MQ=255)

                                                                       cTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGGGCAGTGCAGTTGGGTGACACGGCGTGCTGCTTTGGCGCGTTTTGGTGTTTGTACCCCGCTAAAAAGCAGTtcgt                                                       >  2:1460482/1‑140 (MQ=255)

                                                                        tCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTtcgtc                                                      <  1:822885/140‑1 (MQ=255)

                                                                            ggCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAgg                                                  <  2:1263651/140‑1 (MQ=255)

                                                                            ggCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAgg                                                  <  2:199787/140‑1 (MQ=255)

                                                                             gCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGc                                                 <  1:978801/140‑1 (MQ=255)

                                                                             gCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGc                                                 <  2:697762/140‑1 (MQ=255)

                                                                                 atatTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCcatc                                             <  1:1460482/140‑1 (MQ=255)

                                                                                        cgcgGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTgtc                                      >  1:1414664/1‑138 (MQ=255)

                                                                                          cgGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAg                                    <  2:1231519/140‑1 (MQ=255)

                                                                                               ggCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCcgcg                               >  1:1401886/1‑140 (MQ=255)

                                                                                               ggCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGGGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCcgcg                               >  1:487862/1‑140 (MQ=255)

                                                                                                gCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGAGTAGTGGAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCgcgc                              >  1:312478/1‑140 (MQ=255)

                                                                                                 cGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCt                             >  1:1404960/1‑140 (MQ=255)

                                                                                                   aTGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCttt                           >  1:1268590/1‑140 (MQ=255)

                                                                                                      aaTTCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAgt                        <  2:588565/140‑1 (MQ=255)

                                                                                                        ttCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAgtgt                      <  1:960308/140‑1 (MQ=255)

                                                                                                         tCCATTTCCGGCGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTa                     >  1:16461/1‑140 (MQ=255)

                                                                                                                    cGTGATGATGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTgc          <  2:963900/140‑1 (MQ=255)

                                                                                                                      tgatgaTGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTgcgc        <  2:1404960/140‑1 (MQ=255)

                                                                                                                       gatgatGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCa       <  1:1405174/140‑1 (MQ=255)

                                                                                                                       gatgatGCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCa       <  2:487862/140‑1 (MQ=255)

                                                                                                                         tgatgCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACg     <  2:1401886/140‑1 (MQ=255)

                                                                                                                            tgCCCTGGCGGGTGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTa  <  1:177752/140‑1 (MQ=255)

ATATTTTCCGGCAGATGTGCGCCAAAGCTCATTCCCGGATGCTGGTGGCGTAAAACCTCGCTACGGATGCGCTCGCGGCCCATATTCTCGCGGATGGCGATGAATTCCATTTCCGGCGTGATGATGCCCTGGCGGGCGTAGTGCAGTTGGGTGACACGGCGTCCTGCTTTGGCGCGTTTTGGTGTTAGTACGCCGCTAAAACGCAGTTCGTCGAGGCCATCATCTGCCAGCCGCGCTTTAGTGTAATCGGAACTGCGCACGGTA  >  NC_000913/4195507‑4195770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A