Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A38 F60 I1 R1	20	100.0	3384686	98.8%	3344069	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,331,208	A→G	K54R (AAG→AGG)	topA →	DNA topoisomerase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,331,208	0	A	G	100.0%	366.7 / NA	94	K54R (AAG→AGG)	topA	DNA topoisomerase 1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/50); total (44/50)

GTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAA  >  NC_000913/1331074‑1331341

gTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACgg                                                                                                                                  >  1:1006675/1‑140 (MQ=255)

   cccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGCGCCGACTCTACCTCCACCAGGACGACTa                                                                                                                               >  2:678285/1‑140 (MQ=255)

    ccGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTaa                                                                                                                              >  2:300003/1‑140 (MQ=255)

     cagCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTaaa                                                                                                                             >  1:175850/3‑140 (MQ=255)

      ggCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTaaaa                                                                                                                            >  1:201562/1‑140 (MQ=255)

           aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGTCTCTACCTCCACCAGGACGGCTAAAAAGCCt                                                                                                                       <  1:161276/140‑1 (MQ=255)

           aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCt                                                                                                                       >  2:331351/1‑140 (MQ=255)

           aaGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCt                                                                                                                       >  1:1456804/1‑140 (MQ=255)

              ccAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTaaa                                                                                                                    <  2:1646345/140‑1 (MQ=255)

                  aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTaaa                                                                                                                             >  1:581375/1‑127 (MQ=255)

                  aaCGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTaaa                                                                                                                             <  2:581375/127‑1 (MQ=255)

                          acaaGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGt                                                                                                        <  1:1194953/140‑1 (MQ=255)

                              gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                                                    >  2:651153/1‑140 (MQ=255)

                              gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                                                    >  1:827882/1‑140 (MQ=255)

                              gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                                                    >  1:252139/1‑140 (MQ=255)

                              gTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGgcg                                                                                                    <  1:1002875/140‑1 (MQ=255)

                                 tCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGctc                                                                                                 >  1:108968/1‑140 (MQ=255)

                                     ggTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGt                                                                                             >  1:1111158/1‑140 (MQ=255)

                                     ggTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGt                                                                                             >  2:1240910/1‑140 (MQ=255)

                                       tAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCa                                                                                           <  2:827882/140‑1 (MQ=255)

                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTCGCATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          <  2:1149060/140‑1 (MQ=255)

                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          <  2:1404313/140‑1 (MQ=255)

                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          <  1:817554/140‑1 (MQ=255)

                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          <  2:201562/140‑1 (MQ=255)

                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          <  2:83068/140‑1 (MQ=255)

                                        aGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGAGCTCTCGTCaa                                                                                          <  1:109293/140‑1 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCTGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGt                                                                                             >  1:301200/1‑136 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCTGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGt                                                                                             <  2:301200/136‑1 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGct                                                                                                  <  1:270832/131‑1 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGct                                                                                                  >  2:270832/1‑131 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          >  2:531446/1‑139 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCaa                                                                                          <  1:531446/139‑1 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAc                                                                                         >  1:940704/1‑140 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAc                                                                                         <  2:108968/140‑1 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAc                                                                                         >  1:426627/1‑140 (MQ=255)

                                         gTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAc                                                                                         >  2:859805/1‑140 (MQ=255)

                                          tGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTc                                                                                            <  2:1430327/136‑1 (MQ=255)

                                          tGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTc                                                                                            >  1:1430327/1‑136 (MQ=255)

                                              tACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                                    <  1:372989/140‑1 (MQ=255)

                                              tACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                                    <  2:877372/140‑1 (MQ=255)

                                               aCGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATg                                                                                   >  1:638567/1‑140 (MQ=255)

                                               aCGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATg                                                                                   >  1:1060676/1‑140 (MQ=255)

                                               aCGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATg                                                                                   >  1:1484506/1‑140 (MQ=255)

                                                cGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATgg                                                                                  >  2:441161/1‑140 (MQ=255)

                                                   ggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGTTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAc                                                                                         >  1:744188/1‑130 (MQ=255)

                                                   ggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGTTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAAc                                                                                         <  2:744188/130‑1 (MQ=255)

                                                   ggtgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATggggg                                                                               >  2:83857/1‑140 (MQ=255)

                                                     tgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGtt                                                                             >  2:964398/1‑140 (MQ=255)

                                                     tgAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGtt                                                                             >  2:1592021/1‑140 (MQ=255)

                                                      gAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTg                                                                            >  2:575535/1‑140 (MQ=255)

                                                          tCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGAcc                                                                         >  2:95267/1‑139 (MQ=255)

                                                          tCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGAcc                                                                         <  1:95267/139‑1 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATTAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  2:1442694/140‑1 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  2:1045731/140‑1 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  2:426627/140‑1 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  2:397077/140‑1 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  2:175850/140‑1 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    >  1:824235/1‑140 (MQ=255)

                                                              gCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  2:1484506/140‑1 (MQ=255)

                                                               cGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTTCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGc                                                                   >  1:113058/1‑140 (MQ=255)

                                                               cGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                                    <  2:329411/123‑1 (MQ=255)

                                                               cGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTAt                                                                                    >  1:329411/1‑123 (MQ=255)

                                                                gTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGca                                                                  <  2:1456804/140‑1 (MQ=255)

                                                                            cgcgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                                      <  1:922878/140‑1 (MQ=255)

                                                                            cgcgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                                      <  1:1592021/140‑1 (MQ=255)

                                                                            cgcgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                                      >  1:310376/1‑140 (MQ=255)

                                                                            cgcgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                                      <  2:638567/140‑1 (MQ=255)

                                                                            cgcgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgc                                                      <  1:964398/140‑1 (MQ=255)

                                                                              cgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    <  1:1137017/124‑1 (MQ=255)

                                                                              cgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTgg                                                                    >  2:1137017/1‑124 (MQ=255)

                                                                              cgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                                                    >  1:1445602/1‑140 (MQ=255)

                                                                              cgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                                                    <  1:199571/140‑1 (MQ=255)

                                                                              cgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                                                    >  1:314720/1‑140 (MQ=255)

                                                                              cgATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGgcgc                                                    >  2:41010/1‑140 (MQ=255)

                                                                               gATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                                                   <  2:1060676/140‑1 (MQ=255)

                                                                               gATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCa                                                   <  1:41010/140‑1 (MQ=255)

                                                                                  ttGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTACACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTa                                                <  2:572044/140‑1 (MQ=255)

                                                                                   tGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAt                                               >  2:786877/1‑140 (MQ=255)

                                                                                   tGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAt                                               >  2:592808/1‑140 (MQ=255)

                                                                                   tGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAt                                               <  2:618175/140‑1 (MQ=255)

                                                                                   tGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTAt                                               <  1:300003/140‑1 (MQ=255)

                                                                                    gCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATg                                              <  2:1006675/140‑1 (MQ=255)

                                                                                    gCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATg                                              <  1:575535/140‑1 (MQ=255)

                                                                                    gCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATg                                              <  2:314720/140‑1 (MQ=255)

                                                                                             tGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGc                                     <  1:1194339/140‑1 (MQ=255)

                                                                                              ggCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGcc                                    <  2:824235/140‑1 (MQ=255)

                                                                                                  cAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGt                                <  1:786877/140‑1 (MQ=255)

                                                                                                             aaGAGTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGgt                     <  1:441161/140‑1 (MQ=255)

                                                                                                               gagTGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGgtcg                   >  2:302483/1‑140 (MQ=255)

                                                                                                                  tGCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGtct                >  1:478593/1‑140 (MQ=255)

                                                                                                                  cgCCGACTCTATCTCCACCAGGACGGCTAAAGTGCCTAAAAAGGATAAACGTGGCGCTCTCGTCAACCGGATGGCGGTGGACCCGTGGAATCGTTGGGACACCCTCTCTGGAGCGCTGCCTGGCAAAGACAAGGACGtct                >  2:371272/2‑140 (MQ=255)

                                                                                                                   gCCGACTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGtctc               <  1:859805/140‑1 (MQ=255)

                                                                                                                       aCTCTACCTCCACCAGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTctgaa           >  2:1034681/1‑140 (MQ=255)

                                                                                                                                ccaccaGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAaacaa  <  1:24347/140‑1 (MQ=255)

                                                                                                                                   ccaGGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAaacaa  <  1:668922/137‑1 (MQ=255)

GTCCCCGGCAAAAGCCAAAACGATCAACAAGTATCTGGGTAGTGACTACGTGGTGAAATCCAGCGTCGGTCACATCCGCGATTTGCCGACCAGTGGCTCAGCTGCCAAAAAGAGTGCCGACTCTACCTCCACCAAGACGGCTAAAAAGCCTAAAAAGGATGAACGTGGCGCTCTCGTCAACCGTATGGGGGTTGACCCGTGGCACAATTGGGAGGCGCACTATGAAGTGTTGCCTGGTAAAGAGAAGGTCGTCTCTGAACTGAAACAA  >  NC_000913/1331074‑1331341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A